Information on EC 5.1.99.3 - Allantoin racemase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
5.1.99.3
-
RECOMMENDED NAME
GeneOntology No.
Allantoin racemase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(S)(+)-Allantoin = (R)(-)-allantoin
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
racemization
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Microbial metabolism in diverse environments
-
-
Purine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
Allantoin racemase
-
CAS REGISTRY NUMBER
COMMENTARY hide
56214-40-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain M5al, gene hpxA is not essential for growth on allantoin
UniProt
Manually annotated by BRENDA team
strain M5al, gene hpxA is not essential for growth on allantoin
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
conversion of allantoin to allantoate is the second of the two ring-opening steps in purine utilization
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-allantoin
(S)-allantoin
show the reaction diagram
(S)(+)-Allantoin
(R)(-)-Allantoin
show the reaction diagram
(S)-allantoin
(R)-allantoin
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S)-allantoin
(R)-allantoin
show the reaction diagram
B5B0K3
-
-
-
r
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
5-Aminohydantoin
-
-
Hydantoin of N-carbamoyl-L-methionine
-
-
iodoacetate
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
-
preincubation for 30 min at 30 C stimulates
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.4
(R)-allantoin
-
in 10 mM NaCl and 10 mM HEPES, pH 7.5, at 25C
0.3
(S)-allantoin
-
in 10 mM NaCl and 10 mM HEPES, pH 7.5, at 25C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
238
(R)-allantoin
Klebsiella pneumoniae subsp. pneumoniae
-
in 10 mM NaCl and 10 mM HEPES, pH 7.5, at 25C
183
(S)-allantoin
Klebsiella pneumoniae subsp. pneumoniae
-
in 10 mM NaCl and 10 mM HEPES, pH 7.5, at 25C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
595
(R)-allantoin
Klebsiella pneumoniae subsp. pneumoniae
-
in 10 mM NaCl and 10 mM HEPES, pH 7.5, at 25C
12997
610
(S)-allantoin
Klebsiella pneumoniae subsp. pneumoniae
-
in 10 mM NaCl and 10 mM HEPES, pH 7.5, at 25C
3029
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 60
-
20C: about 35% of maximal activity, 30C: 67% of maximal activity, 60C: about 85% of maximal activity
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25578
x * 25578, calculated
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 24-34% (w/v) polyethylene glycol 400 with 0.1 M sodium acetate, pH 3.7-4.1
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7
-
up to 70C, 15 min, stable
2445
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
-
15 min, pH 6.5-7, stable up to
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
repeated freezing and thawing inactivates
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, stable for several weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA resin column chromatography, gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli B834(DE3) cells
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme belong to a 23-gene cluster that encodes the enzymes for utilizing purines as the sole nitrogen source
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C184S
-
the mutation leads to total loss of activity
C79S
-
the mutation leads to total loss of activity
C79S/C184S
-
the double mutation leads to total loss of activity
C184S
-
the mutation leads to total loss of activity
-
C79S
-
the mutation leads to total loss of activity
-
C79S/C184S
-
the double mutation leads to total loss of activity
-
C184S
-
the mutation leads to total loss of activity
-
C79S
-
the mutation leads to total loss of activity
-
C79S/C184S
-
the double mutation leads to total loss of activity
-
additional information