Information on EC 5.1.3.35 - 2-epi-5-epi-valiolone 7-phosphate 2-epimerase

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The expected taxonomic range for this enzyme is: Actinoplanes

EC NUMBER
COMMENTARY hide
5.1.3.35
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RECOMMENDED NAME
GeneOntology No.
2-epi-5-epi-valiolone 7-phosphate 2-epimerase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-epi-5-epi-valiolone 7-phosphate = 5-epi-valiolone 7-phosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Acarbose and validamycin biosynthesis
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Biosynthesis of antibiotics
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SYSTEMATIC NAME
IUBMB Comments
2-epi-5-epi-valiolone-7-phosphate 2-epimerase
The enzyme, isolated from the bacterium Actinoplanes sp. SE 50/110, is involved in the biosynthesis of the alpha-glucosidase inhibitor acarbose.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene acbO
UniProt
Manually annotated by BRENDA team
gene acbO
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-epi-5-epi-valiolone 7-phosphate
5-epi-valiolone 7-phosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-epi-5-epi-valiolone 7-phosphate
5-epi-valiolone 7-phosphate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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the purfied recombinant His-tagged AcbO protein is actively converting the product of the AcbM-catalysed reaction in absence of any other cofactor or coenzyme
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.6
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29500
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x * 29500, recombinant His-tagged enzyme, SDS-PAGE, x * 30616, sequence calculation
30616
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x * 29500, recombinant His-tagged enzyme, SDS-PAGE, x * 30616, sequence calculation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Streptomyces lividans strain TK64 by nickel affinity chromatgraphy
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene acbO, encoded in the acbKMLNOC operon, DNa and amino acid sequence determination and analysis, overexpression of N-terminally His-tagged enzyme in Streptomyces lividans strain TK64, expression in Escherichia coli is not successful, method development, overview
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gene acbO, encoded in the acbKMLNOC operon, overexpression of N-terminally His-tagged enzyme in Streptomyces lividans strain TK64 under the control of an inducible promoter (ptipA)
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