Information on EC 5.1.3.34 - monoglucosyldiacylglycerol epimerase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
5.1.3.34
-
RECOMMENDED NAME
GeneOntology No.
monoglucosyldiacylglycerol epimerase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol = a 1,2-diacyl-3-O-(beta-D-galactopyranosyl)-sn-glycerol
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
galactolipid biosynthesis II
-
-
Glycerolipid metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol 4-epimerase
The enzyme, characterized from cyanobacteria, is involves in the biosynthesis of galactolipids found in their photosynthetic membranes.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain ATCC 29413; gene mgdE
UniProt
Manually annotated by BRENDA team
strain ATCC 29413; gene mgdE
UniProt
Manually annotated by BRENDA team
no activity in Acaryochloris marina
-
-
-
Manually annotated by BRENDA team
no activity in Acaryochloris marina MBIC11017
-
-
-
Manually annotated by BRENDA team
no activity in Gloeobacter violaceus
-
-
-
Manually annotated by BRENDA team
no activity in Gloeobacter violaceus PCC7421
-
-
-
Manually annotated by BRENDA team
no activity in Thermosynechococcus elongatus
-
-
-
Manually annotated by BRENDA team
no activity in Thermosynechococcus elongatus BP1
-
-
-
Manually annotated by BRENDA team
gene mgdE
UniProt
Manually annotated by BRENDA team
gene mgdE
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
knockout mutants of mgdE in Synechocystis sp. PCC 6803 lack both monogalactosyldiacylglycerol and digalactosyldiacylglycerol and accumulated monoglucosyldiacylglycerol. The mutants possess thylakoid membranes and show normal maximal photosynthetic activity, albeit with reduced utilization of light energy. Cyanobacterial mutants in which the gene is disrupted still grow photosynthetically, indicating that galactolipids are not essential for either oxygenic photosynthesis or the development of the thylakoid membrane structure. Phenotype, overview
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
a 1,2-diacyl-3-O-(berta-D-glucopyranosyl)-sn-glycerol
a 1,2-diacyl-3-O-(beta-D-galactopyranosyl)-sn-glycerol
show the reaction diagram
a 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol
a 1,2-diacyl-3-O-(beta-D-galactopyranosyl)-sn-glycerol
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
a 1,2-diacyl-3-O-(berta-D-glucopyranosyl)-sn-glycerol
a 1,2-diacyl-3-O-(beta-D-galactopyranosyl)-sn-glycerol
show the reaction diagram
a 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol
a 1,2-diacyl-3-O-(beta-D-galactopyranosyl)-sn-glycerol
show the reaction diagram
additional information
?
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene mgdE, recombinant expression in Escherichia coli strain BL21 (DE3), co-expression with Prochlorococcus marinus gene mgdA, the transformed Escherichia coli cells are temperature-sensitive and do not accumulate neither monogalactosyldiacylglycerol nor digalactosyldiacylglycerol when grown at 37°C
-
gene mgdE, sequence comparisons, recombinant expression in wild-type Synechocystis sp. PCC 6803 or the strain overexpressing CsMGD1 under the control of the light-inducible psbA promoter
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
generation of mgdE gene disruption mutants, disruption of the gene for the glucolipid epimerase (mgdE) in the monogalactosyldiacylglycerol (MGDG) synthase gene 1 (MGD1) background. The DELTAmgdE mutants retain about 70% of the chlorophyll content of the wild-type, no galactose moiety is detected in the deletion mutant by GC-MS analysis, confirming that monogalactosyldiacylglycerol comprise less than 0.4% of total membrane lipids. The mutants contain normal-looking thylakoid membranes. TLC analysis of lipids in the wild-type and DELTAmgdE cells of Synechocystis sp. PCC 6803 expressing GST-MGD1