Information on EC 5.1.3.21 - maltose epimerase

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The expected taxonomic range for this enzyme is: Lactobacillus brevis

EC NUMBER
COMMENTARY hide
5.1.3.21
-
RECOMMENDED NAME
GeneOntology No.
maltose epimerase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
alpha-maltose = beta-maltose
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
isomerization
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
maltose 1-epimerase
The enzyme catalyses the interconversion of alpha and beta anomers of maltose more effectively than those of disaccharides such as lactose and cellobiose.
CAS REGISTRY NUMBER
COMMENTARY hide
166799-98-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
IFO 3345
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-D-Glucose
beta-D-Glucose
show the reaction diagram
-
at 26% of the activity with beta-maltose
-
-
r
alpha-lactose
beta-lactose
show the reaction diagram
-
at 5% of the activity with beta-maltose
-
-
?
beta-cellobiose
alpha-cellobiose
show the reaction diagram
-
at 4% of the activity with beta-maltose
-
-
?
beta-D-glucose
alpha-D-glucose
show the reaction diagram
-
at 27% of the activity with beta-maltose
-
-
r
beta-Maltose
alpha-Maltose
show the reaction diagram
-
-
-
r
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.2
beta-maltose
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8.5
-
more than 80% of activity in the pH-range pH 5.0-8.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43000
-
gel filtration
45000
-
1 * 45000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 45000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
-
10 min, 50 mM phosphate buffer, stable
60
-
10 min, 50 mM phosphate buffer, 50% loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Show AA Sequence (238 entries)
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