Information on EC 5.1.3.20 - ADP-glyceromanno-heptose 6-epimerase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
5.1.3.20
-
RECOMMENDED NAME
GeneOntology No.
ADP-glyceromanno-heptose 6-epimerase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ADP-D-glycero-D-manno-heptose = ADP-L-glycero-D-manno-heptose
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
epimerization
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ADP-L-glycero-beta-D-manno-heptose biosynthesis
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Lipopolysaccharide biosynthesis
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
ADP-L-glycero-D-manno-heptose 6-epimerase
Requires NAD+.
CAS REGISTRY NUMBER
COMMENTARY hide
130527-81-0
epimerase, adenosine diphosphoglyceromannoheptose 6- (Escherichia coli clone pCG50 reduced) /ADP-L-glycero-D-mannoheptose-6-epimerase (Escherichia coli strain K-12 substrain MG1655 clone EC19-98 gene rfaD) /genBank AE000440-derived protein GI 1790049
167617-20-1
epimerase, adenosine diphosphoglyceromannoheptose 6- (Neisseria gonorrhoeae gene lis-6), ADP-L-glycero-D-mannoheptose 6-epimerase (Neisseria gonorrhoeae gene gme)
204790-62-5
ADP-L-glycero-D-manno-heptose-epimerase, Aquifex aelicis gene rfaD /GenBank AE000684-derived protein GI 2983012
85030-75-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene rfaD
W6C0E3
UniProt
Manually annotated by BRENDA team
gene rfaD
W6C0E3
UniProt
Manually annotated by BRENDA team
strain 2019
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-
Manually annotated by BRENDA team
strain 2019
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-
Manually annotated by BRENDA team
gene HP0859 or rfaD
UniProt
Manually annotated by BRENDA team
gene HP0859 or rfaD
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP-beta-D-glycero-D-manno-heptose
ADP-beta-L-glycero-D-mannoheptose
show the reaction diagram
-
-
-
-
?
ADP-beta-D-manno-hexadialdose
ADP-beta-D-mannuronic acid + ADP-beta-D-mannose
show the reaction diagram
-
the intermediate ADP-beta-D-manno-hexadialdose undergoes dismutation into equal amounts of ADP-beta-D-mannuronic acid and ADP-beta-D-mannose
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-
?
ADP-D-glycero-D-manno-heptose
?
show the reaction diagram
ADP-D-glycero-D-manno-heptose
ADP-L-glycero-D-manno-heptose
show the reaction diagram
ADP-D-glycero-D-mannoheptose
ADP-L-glycero-D-mannoheptose
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ADP-beta-D-glycero-D-manno-heptose
ADP-beta-L-glycero-D-mannoheptose
show the reaction diagram
-
-
-
-
?
ADP-D-glycero-D-manno-heptose
?
show the reaction diagram
ADP-D-glycero-D-manno-heptose
ADP-L-glycero-D-manno-heptose
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
additional information
preference of the enzyme for NAD+ compared to NADP+. When NADP is bound, the binding region assumes a different conformation
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ADP-glucose
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ADPglucose
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1
ADP-D-glycero-D-manno-heptose
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00087
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-
2.7
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at 23C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 9.5
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-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 55
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5C: about 45% of maximal activity, 55C: about 50% of maximal activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8
sequence calculation
PDB
SCOP
CATH
ORGANISM
UNIPROT
Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Helicobacter pylori (strain G27)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35000
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x * 35000, calculation from nucleotide sequence
37000
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6 * 37000, SDS-PAGE
38000
W6C0E3
x * 38000, recombinant His6-tagged enzyme, SDS-PAGE
230000 - 250000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
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6 * 37000, SDS-PAGE
pentamer
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
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-
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified recombinant His-tagged enzyme, hanging drop vapour diffusion method, mixing of 200 nl of 8.8 mg/ml protein in 0.43 M NaCl, 20 mM Tris-HCl, pH 8.0, with 200 nl of reservoir solution containing 35% PEG 200, 0.1 M bis-tris-HCl, pH 5.5, and equilibration against 0.05 ml of reservori solution, method optimization, X-ray diffraction structure determination and analysis at 2.6 A resolution, molecular replacement
W6C0E3
hanging-drop vapor-diffusion method, three crystal forms
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structure of the enzyme in complex with NADP+ and the inhibitor ADP-glucose, determined at 2.0 A resolution by multiwavelength anomalous diffraction phasing method
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Y140F mutant enzyme bound to ADP-beta-D-mannose, hanging drop vapor diffusion method, using 2M (NH4)2SO4, 0.1 M HEPES-Na, pH 7.1, 2% (w7v) PEG 400
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purified recombinant His-tagged enzyme, vapor diffusion technique, mixing of 20 mg/ml protein in 30 mM Tris, pH 7.5, 150 mM NaCl, with precipitant solution containing 0.2 M ammonium sulfate, 0.1 M tri sodium citrate, pH 5.6, 15% w/v PEG 4000, and 5% glycerol, 20C, X-ray diffraction structure determination and analysis at 2.55 A resolution
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
metal affinity column chromatography, hydrophobic affinity column chromatography, and gel filtration
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recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gell filtration
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography
W6C0E3
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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gene HP0859, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene rfaD, sequence compariosns, expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
W6C0E3
identification of colicin E1 hybrid plasmid carrying the coding sequence for ADP-L-glycero-D-mannoheptose-6-epimerase from Clarke-carbon bank
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mutant enzyme Y140F is expressed in Escherichia coli BL21(DE3) cells
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overexpression in Escherichia coli
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Plasmids encoding hexahistidine-tagged mutant and wildtype enzymes overexpressed in Escherichia coli.
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E210G
W6C0E3
site-directed mutagenesis, structure compared to the wild-type enzyme
K178M
W6C0E3
site-directed mutagenesis, structure compared to the wild-type enzyme
E210G
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site-directed mutagenesis, structure compared to the wild-type enzyme
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K178M
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site-directed mutagenesis, structure compared to the wild-type enzyme
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D210N
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mutant display activity similar to that of the wild type
K178M
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mutant has severely compromised epimerase activities that are more than 3 orders of magnitude lower than that of the wild type
K208M
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mutant display activity similar to that of the wild type
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