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Information on EC 5.1.1.4 - proline racemase and Organism(s) Clostridioides difficile and UniProt Accession Q17ZY4

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EC Tree
     5 Isomerases
         5.1 Racemases and epimerases
             5.1.1 Acting on amino acids and derivatives
                5.1.1.4 proline racemase
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This record set is specific for:
Clostridioides difficile
UNIPROT: Q17ZY4 not found.
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Word Map
The taxonomic range for the selected organisms is: Clostridioides difficile
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
Synonyms
proline racemase, tcprac, tvprac, prac1, tcpraca, tryprac, tcpracb, proline racemase a, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Racemase, proline
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
racemization
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
proline racemase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9024-09-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-proline
D-proline
show the reaction diagram
-
-
-
r
L-proline
D-proline
show the reaction diagram
CdPRAC from Clostridium difficile racemizes both L- and D-Pro but not OH-L/D-Pro or any other natural amino acid
-
-
r
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyrrole-2-carboxylate
-
pyrrole-2-carboxylic acid
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.612
D-proline
pH 8.0, 50°C, wild-type enzyme
8.53
L-proline
pH 8.0, 50°C, wild-type enzyme
46.7 - 60.2
(25R)-3beta-hydroxycholest-5-en-27-oate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.48
D-proline
pH 8.0, 50°C, wild-type enzyme
14.15
L-proline
pH 8.0, 50°C, wild-type enzyme
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.08
D-proline
pH 8.0, 50°C, wild-type enzyme
1.65
L-proline
pH 8.0, 50°C, wild-type enzyme
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.269
pyrrole-2-carboxylate
Clostridioides difficile
pH 8.0, 50°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
inactivation of proline racemase PrdF by insertional mutagenesis does not affect early logarithmic growth but only attenuates growth in the mid- and late logarithmic phases. There is no effect of inactivation on virulence in vivo
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
x * 40000, SDS-PAGE
80000
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 40000, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
cloned in Escherichia coli as a 6xHis-tag fusion protein
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Goytia, M.; Chamond, N.; Cosson, A.; Coatnoan, N.; Hermant, D.; Berneman, A.; Minoprio, P.
Molecular and structural discrimination of proline racemase and hydroxyproline-2-epimerase from nosocomial and bacterial pathogens
PLoS ONE
2
e885
2007
Clostridioides difficile (A8DEZ8), Clostridioides difficile VPI10463 (A8DEZ8)
Manually annotated by BRENDA team
Wu, X.; Hurdle, J.G.
The Clostridium difficile proline racemase is not essential for early logarithmic growth and infection
Can. J. Microbiol.
60
251-254
2014
Clostridioides difficile
Manually annotated by BRENDA team
Watanabe, S.; Tanimoto, Y.; Nishiwaki, H.; Watanabe, Y.
Identification and characterization of bifunctional proline racemase/hydroxyproline epimerase from archaea: discrimination of substrates and molecular evolution
PLoS One
10
e0120349
2015
Clostridioides difficile (Q17ZY4), Ferroplasma acidarmanus (S0APF4), Haloarcula japonica (M0LMI3), Haloarcula japonica DSM 6131 (M0LMI3), Thermococcus litoralis (H3ZMH5), Thermococcus litoralis, Thermococcus litoralis DSM 5473 (H3ZMH5)
Manually annotated by BRENDA team