Information on EC 4.99.1.6 - indoleacetaldoxime dehydratase

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY hide
4.99.1.6
-
RECOMMENDED NAME
GeneOntology No.
indoleacetaldoxime dehydratase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(indol-3-yl)acetaldehyde oxime = (indol-3-yl)acetonitrile + H2O
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
camalexin biosynthesis
-
-
indole-3-acetate biosynthesis II
-
-
Tryptophan metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
(indol-3-yl)acetaldehyde-oxime hydro-lyase [(indol-3-yl)acetonitrile-forming]
-
CAS REGISTRY NUMBER
COMMENTARY hide
9024-27-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
var. cubense
-
-
Manually annotated by BRENDA team
banana
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(E,Z)-3-(3-indolyl)propanal oxime
?
show the reaction diagram
51% activity compared to (E,Z)-indolyl-3-acetaldoxime
-
-
?
(E,Z)-4-(3-indolyl)butanal oxime
?
show the reaction diagram
12% activity compared to (E,Z)-indolyl-3-acetaldoxime
-
-
?
(E,Z)-4-hydroxyphenylacetaldehyde oxime
?
show the reaction diagram
30% activity compared to (E,Z)-indolyl-3-acetaldoxime
-
-
?
(E,Z)-4-methoxyphenylacetaldoxime
?
show the reaction diagram
19% activity compared to (E,Z)-indolyl-3-acetaldoxime
-
-
?
(E,Z)-indolyl-3-acetaldoxime
(E,Z)-indolyl-3-acetonitrile
show the reaction diagram
100% activity
-
-
r
3-Indoleacetaldoxime
3-Indoleacetonitrile
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe3+
-
Ferric citrate promotes activity
KCN
-
activates at 0.001-0.1 mM, inhibits at 1-10 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,3-Dimercaptopropanol
2-mercaptoethanol
-
-
8-hydroxyquinoline
-
inhibition is partly reversed by ferric citrate, ascorbic acid and dehydroascorbic acid
Ag+
-
1 mM, complete inactivation
Al3+
-
1 mM, complete inactivation
Benzaldoxime
-
-
Cu2+
-
1 mM, complete inactivation
Diethyl dithiocarbamate
-
-
Hg2+
-
1 mM, complete inactivation
Mandelaldoxime
-
-
Mercaptoacetic acid
-
weak
Mo5+
-
1 mM, complete inactivation
NaBH4
-
inhibition is partly reversed by pyridoxal-5'-phosphate or dehydroascorbic acid
p-hydroxymercuribenzoate
-
-
phenylacetaldoxime
-
competitive
Phenylpropionaldoxime
-
-
Phenylthiocyanate
-
-
tetrahydrofolic acid
-
-
Zn2+
-
1 mM, complete inactivation
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dehydroascorbic acid
-
activates
dihydrofolic acid
-
activates
glutathione
-
activates
Na2S2O4
the specific activity of IADSs increases about 17fold upon addition of Na2S2O4 under anaerobic conditions
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.29
(E,Z)-indolyl-3-acetaldoxime
in 25 mM Tris-HCl buffer pH 7.5, at 23C
0.17
3-indoleacetaldoxime
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
10
(E,Z)-indolyl-3-acetaldoxime
Sclerotinia sclerotiorum
A7E8M5
in 25 mM Tris-HCl buffer pH 7.5, at 23C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0045
crude extract, in 25 mM Tris-HCl buffer pH 7.5, at 23C
0.175
after 39fold purification, in 25 mM Tris-HCl buffer pH 7.5, at 23C
additional information
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
-
pH 6.0: about 60% of maximal activity, pH 9.0: about 35% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
calculated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41000
calculated from amino acid sequence
44000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
1 * 44000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7
-
maximally stable
648796
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hydroxyapatite column chromatography, DEAE Sephacel gel filtration, and Superdex G-75 gel filtration