Information on EC 4.6.1.6 - cytidylate cyclase

New: Word Map on EC 4.6.1.6
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
4.6.1.6
-
RECOMMENDED NAME
GeneOntology No.
cytidylate cyclase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
CTP = 3',5'-cyclic CMP + diphosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
P-O bond cleavage
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
CTP diphosphate-lyase (cyclizing; 3',5'-cyclic-CMP-forming)
-
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3',5'-cyclic-CMP synthase
-
-
-
-
cytidyl cyclase
-
-
-
-
cytidylyl cyclase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
65357-82-6
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
mouse, strain BAGG-Swiss
-
-
Manually annotated by BRENDA team
rat; strain Lister Hooded
-
-
Manually annotated by BRENDA team
rat; strain Wistar
-
-
Manually annotated by BRENDA team
Rattus norvegicus Lister Hooded
strain Lister Hooded
-
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar
strain Wistar
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CTP
3',5'-cyclic CMP + diphosphate
show the reaction diagram
-
-
-
-
?
CTP
3',5'-cyclic CMP + diphosphate
show the reaction diagram
-
-
-
-
?
CTP
3',5'-cyclic CMP + diphosphate
show the reaction diagram
-
-
-
-
?
CTP
3',5'-cyclic CMP + diphosphate
show the reaction diagram
-
cCMP biosynthesis
-
-
?
CTP
3',5'-cyclic CMP + diphosphate
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
?
CTP
3',5'-cyclic CMP + diphosphate
show the reaction diagram
Rattus norvegicus Lister Hooded
-
-
-
-
?
additional information
?
-
-
distinct from purine nucleotide cyclases, adenylate cyclase and guanylate cyclase
-
-
-
additional information
?
-
-
cyclic CMP is the major product, beside this cytidine 3',5'-cyclic diphosphate, cytidine 2'-monophosphate 3',5'cyclic monophosphate, 2'-O-glutamyl cytidine 3',5'-cyclic monophosphate, and 2'-O-aspartyl cytidine 3',5'-cyclic monophosphate are found
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CTP
3',5'-cyclic CMP + diphosphate
show the reaction diagram
-
-
-
-
?
CTP
3',5'-cyclic CMP + diphosphate
show the reaction diagram
-
-
-
-
?
CTP
3',5'-cyclic CMP + diphosphate
show the reaction diagram
-
cCMP biosynthesis
-
-
?
CTP
3',5'-cyclic CMP + diphosphate
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
?
CTP
3',5'-cyclic CMP + diphosphate
show the reaction diagram
Rattus norvegicus Lister Hooded
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Fe2+
-
cyclic CMP formation is enhanced by 0.3 mM
Mg2+
-
complete loss of activity in absence of Mg2+ or Mn2+
Mg2+
-
required
Mn2+
-
cyclic CMP formation is enhanced by 0.3 mM
Mn2+
-
required
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
9-[2-(phosphonomethoxy)ethyl]adenine diphosphate
-
-
ATP
-
enzyme dispersed from the membrane fraction of brain
deoxycholate
-
-
L-alpha-Phosphatidic acid
-
-
L-alpha-Phosphatidylethanolamine
-
-
L-alpha-Phosphatidylserine
-
-
methylanthraniloyl-ATP
-
-
methylanthraniloyl-CTP
-
-
methylanthraniloyl-GTP
-
-
methylanthraniloyl-ITP
-
-
methylanthraniloyl-UTP
-
-
Mn2+
-
above 3 mM
additional information
-
enzymatic reaction is inhibited by boiling or detergents
-
additional information
-
no inhibition with L-alpha-phosphatidylcholine
-
additional information
-
enzyme dispersed from the membrane fraction of brain is not inhibited by GTP, also forskolin and lanthanum chloride shows no effect
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Guanine nucleotide-binding protein
-
stimulation of cytidylate cyclase activity via activation of a guanine nucleotide-binding protein
-
L-alpha-phosphatidylcholine
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0125
CTP
-
in the presence of 5 mM Mn2+, in 75 mM HEPES-NaOH, pH 7.4, at 37C
0.0156
CTP
-
-
0.0686
CTP
-
in the presence of 5 mM Mn2+, in 75 mM HEPES-NaOH, pH 7.4, at 37C
0.16
CTP
-
pH 7.4, 37C
0.22
CTP
-
pH 7.4, 37C
0.4197
CTP
-
in the presence of 5 mM Mg2+, in 75 mM HEPES-NaOH, pH 7.4, at 37C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.3
CTP
-
in the presence of 5 mM Mn2+, in 75 mM HEPES-NaOH, pH 7.4, at 37C
7.2
CTP
-
in the presence of 5 mM Mg2+, in 75 mM HEPES-NaOH, pH 7.4, at 37C
8.8
CTP
-
in the presence of 5 mM Mn2+, in 75 mM HEPES-NaOH, pH 7.4, at 37C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
4.37
CTP
-
in the presence of 5 mM Mn2+, in 75 mM HEPES-NaOH, pH 7.4, at 37C
60
17.2
CTP
-
in the presence of 5 mM Mg2+, in 75 mM HEPES-NaOH, pH 7.4, at 37C
60
704
CTP
-
in the presence of 5 mM Mn2+, in 75 mM HEPES-NaOH, pH 7.4, at 37C
60
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0000011
9-[2-(phosphonomethoxy)ethyl]adenine diphosphate
-
in 75 mM HEPES-NaOH, pH 7.4, at 37C
0.0000045
9-[2-(phosphonomethoxy)ethyl]adenine diphosphate
-
in 75 mM HEPES-NaOH, pH 7.4, at 37C
0.00058
methylanthraniloyl-ATP
-
in 75 mM HEPES-NaOH, pH 7.4, at 37C
0.0044
methylanthraniloyl-ATP
-
in 75 mM HEPES-NaOH, pH 7.4,at 37C
0.00008
methylanthraniloyl-CTP
-
in 75 mM HEPES-NaOH, pH 7.4, at 37C
0.0013
methylanthraniloyl-CTP
-
in 75 mM HEPES-NaOH, pH 7.4, at 37C
0.0028
methylanthraniloyl-GTP
-
in 75 mM HEPES-NaOH, pH 7.4, at 37C
0.0061
methylanthraniloyl-GTP
-
in 75 mM HEPES-NaOH, pH 7.4, at 37C
0.001
methylanthraniloyl-ITP
-
in 75 mM HEPES-NaOH, pH 7.4, at 37C
0.0057
methylanthraniloyl-ITP
-
in 75 mM HEPES-NaOH, pH 7.4, at 37C
0.0018
methylanthraniloyl-UTP
-
in 75 mM HEPES-NaOH, pH 7.4, at 37C
0.0038
methylanthraniloyl-UTP
-
in 75 mM HEPES-NaOH, pH 7.4, at 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5
-
liver enzyme
9.4
-
enzyme dispersed from the membrane fraction of brain
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.5 - 9.5
-
about 35% of activity maximum at pH 5.5, about 55% of activity maximum at pH 9.5
6 - 8.5
-
-
7.4 - 9.4
-
20% of activity maximum at physiological pH 7.4, maximal activity at pH 9.4, enzyme dispersed from the membrane fraction of brain
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4 - 45
-
-
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Rattus norvegicus Wistar, Rattus norvegicus Lister Hooded
-
-
-
Manually annotated by BRENDA team
Rattus norvegicus Lister Hooded
-
-
-
Manually annotated by BRENDA team
Rattus norvegicus Lister Hooded
-
-
-
Manually annotated by BRENDA team
Rattus norvegicus Lister Hooded
-
-
-
Manually annotated by BRENDA team
Rattus norvegicus Lister Hooded
-
-
-
Manually annotated by BRENDA team
Rattus norvegicus Lister Hooded
-
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Rattus norvegicus Wistar
-
membrane-bound
-
Manually annotated by BRENDA team
Rattus norvegicus Lister Hooded
-
-
-
Manually annotated by BRENDA team