Any feedback?
Information on EC 4.6.1.6 - cytidylate cyclase
for references in articles please use BRENDA:EC4.6.1.6Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
4.6.1.6 cytidylate cyclaseIUBMB Comments
In bacteria and archaea the enzyme's product, cCMP, functions as a second messenger in bacterial immunity against viruses. The enzyme is synthesized following phage infection and activates immune effectors that execute an antiviral response.
Specify your search results
Word Map
- 4.6.1.6
- cytidine
- 5'-triphosphate
- gtp
-
3',5'-monophosphate
- triton
- pyrophosphate
-
immunoassay
-
ignarro
-
radiometric
- detergent
-
bombardment
- toxin
- adenylate
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
cytidylate cyclase, cytidylyl cyclase, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
3',5'-cyclic-CMP synthase
-
-
-
-
cytidyl cyclase
-
-
-
-
cytidylyl cyclase
cytidylyl cyclase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
CTP = 3',5'-cyclic CMP + diphosphate
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
P-O bond cleavage
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
CTP diphosphate-lyase (cyclizing; 3',5'-cyclic-CMP-forming)
In bacteria and archaea the enzyme's product, cCMP, functions as a second messenger in bacterial immunity against viruses. The enzyme is synthesized following phage infection and activates immune effectors that execute an antiviral response.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
65357-82-6
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
CTP
3',5'-cyclic CMP + diphosphate
-
-
-
-
?
additional information
?
-
-
distinct from purine nucleotide cyclases, adenylate cyclase and guanylate cyclase
-
-
?
additional information
?
-
-
cyclic CMP is the major product, beside this cytidine 3',5'-cyclic diphosphate, cytidine 2'-monophosphate 3',5'cyclic monophosphate, 2'-O-glutamyl cytidine 3',5'-cyclic monophosphate, and 2'-O-aspartyl cytidine 3',5'-cyclic monophosphate are found
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
CTP
3',5'-cyclic CMP + diphosphate
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Fe2+
Mg2+
Mn2+
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
enzymatic reaction is inhibited by boiling or detergents
-
additional information
-
enzyme dispersed from the membrane fraction of brain is not inhibited by GTP, also forskolin and lanthanum chloride shows no effect
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Guanine nucleotide-binding protein
-
stimulation of cytidylate cyclase activity via activation of a guanine nucleotide-binding protein
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Cholera
Cytidylate cyclase activity is stimulated via activation of a guanine nucleotide-binding protein.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0125
CTP
-
in the presence of 5 mM Mn2+, in 75 mM HEPES-NaOH, pH 7.4, at 37°C
0.0686
CTP
-
in the presence of 5 mM Mn2+, in 75 mM HEPES-NaOH, pH 7.4, at 37°C
0.4197
CTP
-
in the presence of 5 mM Mg2+, in 75 mM HEPES-NaOH, pH 7.4, at 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.3
CTP
-
in the presence of 5 mM Mn2+, in 75 mM HEPES-NaOH, pH 7.4, at 37°C
7.2
CTP
-
in the presence of 5 mM Mg2+, in 75 mM HEPES-NaOH, pH 7.4, at 37°C
8.8
CTP
-
in the presence of 5 mM Mn2+, in 75 mM HEPES-NaOH, pH 7.4, at 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
4.37
CTP
-
in the presence of 5 mM Mn2+, in 75 mM HEPES-NaOH, pH 7.4, at 37°C
17.2
CTP
-
in the presence of 5 mM Mg2+, in 75 mM HEPES-NaOH, pH 7.4, at 37°C
704
CTP
-
in the presence of 5 mM Mn2+, in 75 mM HEPES-NaOH, pH 7.4, at 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0000011
9-[2-(phosphonomethoxy)ethyl]adenine diphosphate
-
in 75 mM HEPES-NaOH, pH 7.4, at 37°C
0.0000011
9-[2-(phosphonomethoxy)ethyl]adenine diphosphate
-
in 75 mM HEPES-NaOH, pH 7.4, at 37°C
0.0000045
9-[2-(phosphonomethoxy)ethyl]adenine diphosphate
-
in 75 mM HEPES-NaOH, pH 7.4, at 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.5 - 9.5
-
about 35% of activity maximum at pH 5.5, about 55% of activity maximum at pH 9.5
7.4 - 9.4
-
20% of activity maximum at physiological pH 7.4, maximal activity at pH 9.4, enzyme dispersed from the membrane fraction of brain
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). Q88CF9_PSEPK
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
951
0
108384
TrEMBL
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Cech, S.Y.; Ignarro, L.J.
Cytidine 3,5-monophosphate (cyclic CMP) formation by homogenates of mouse liver
Biochem. Biophys. Res. Commun.
80
119-125
1978
Mus musculus
brenda
Newton, R.P.; Salih, S.A.G.; Hakeem, N.A.; Kingston, E.E.; Beynon, J.H.
3',5'-cyclic UMP, -cyclic IMP, -cyclic TMP and related enzymes in mammalian tissue
Biochem. Soc. Trans.
13
1134-1135
1985
Rattus norvegicus, Rattus norvegicus Lister Hooded
-
brenda
Newton, R.P.; Kingston, E.E.; Hakeem, N.A.; Salih, S'A.G.; Beynon, J.H.; Moyse, C.D.
Extraction, purification, identification and metabolism of 3',5'-cyclic UMP, 3',5'-cyclic IMP, and 3',5'-cyclic dTMP from rat tissues
Biochem. J.
236
431-439
1986
Rattus norvegicus
brenda
Newton, R.P.; Kingston, E.E.; Salvage, B.J.; Beynon, J.H.; Hakeem, N.A.; Wassenaar, T.
Identification of the products of putative cytidylate cyclase activity
Biochem. Soc. Trans.
14
964-965
1986
Rattus norvegicus
-
brenda
Mori, S.; Muto, N.; Yamamoto, I.
Interaction of rat brain cytidylate cyclase with phospholipids
Biochem. Biophys. Res. Commun.
162
1502-1507
1989
Rattus norvegicus, Rattus norvegicus Wistar
brenda
Mori, S.; Yanagida, M.; Kubotsu, K.; Yamamoto, I.
Characterization of detergent dispersed cytidylate cyclase of rat brain
Second Messengers Phosphoproteins
13
1-12
1990
Rattus norvegicus
brenda
Newton, R.P.; Salvage, B.J.; Hakeem, N.A.
Cytidylate cyclase: development of assay and determination of kinetic properties of a cytidine 3,5-cyclic monophosphate-synthesizing enzyme
Biochem. J.
265
581-586
1990
Rattus norvegicus
brenda
Muto, N.; Kanoh, M.; Yamamoto, I.
Cytidylate cyclase activity is stimulated via activation of a guanine nucleotide-binding protein
Life Sci.
52
13-20
1993
Rattus norvegicus
brenda
Newton, R.P.; Groot, N.; van Geyschem, J.; Diffley, P.E.; Walton, T.J.; Bayliss, M.A.; Harris, F.M.; Games, D.E.; Brenton, A.G.
Estimation of cytidylyl cyclase activity and monitoring of side-product formation by fast-atom bombardment mass spectrometry
Rapid Commun. Mass. Spectrom.
11
189-194
1997
Rattus norvegicus, Rattus norvegicus Lister Hooded
brenda
Goettle, M.; Dove, S.; Kees, F.; Schlossmann, J.; Geduhn, J.; Koenig, B.; Shen, Y.; Tang, W.J.; Kaever, V.; Seifert, R.
Cytidylyl and uridylyl cyclase activity of Bacillus anthracis edema factor and Bordetella pertussis CyaA
Biochemistry
49
5494-5503
2010
Bacillus anthracis, Bordetella pertussis
brenda
Select items on the left to see more content.
EXTERNAL LINKS (specific for EC-Number 4.6.1.6)
html completed
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
About BRENDA
Social media
Help
Survey
Download
Contribution
Legal
Curated at
Member of
