Information on EC 4.6.1.2 - guanylate cyclase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
4.6.1.2
-
RECOMMENDED NAME
GeneOntology No.
guanylate cyclase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
GTP = 3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
GTP = 3',5'-cyclic GMP + diphosphate
show the reaction diagram
reaction mechanism, structure-function relationship
-
GTP = 3',5'-cyclic GMP + diphosphate
show the reaction diagram
reaction mechanism, structure-function relationship
Q8SPV3
GTP = 3',5'-cyclic GMP + diphosphate
show the reaction diagram
reaction mechanism, structure-function relationship
-
GTP = 3',5'-cyclic GMP + diphosphate
show the reaction diagram
reaction mechanism, overview; reaction mechanism, overview
P16068, P19687
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
P-O bond cleavage
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Purine metabolism
-
SYSTEMATIC NAME
IUBMB Comments
GTP diphosphate-lyase (cyclizing; 3',5'-cyclic-GMP-forming)
Also acts on ITP and dGTP.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
AC-IV
Q7CH76
-
alpha1 sGC
-
-
alpha1 soluble guanylyl cyclase
-
-
alpha1beta1 guanylyl cyclase
-
-
alpha1beta1 sGC
-
-
ANF-RGC
-
-
ANPRA
-
-
-
-
ANPRB
-
-
-
-
atrial natriuretic factor receptor guanylate cyclase
-
-
Atrial natriuretic peptide A-type receptor
-
-
-
-
Atrial natriuretic peptide B-type receptor
-
-
-
-
brassinosteroid receptor 1
-
-
class IV adenylyl cyclase
Q7CH76
-
CNP-sensitive guanyly cyclase
-
-
CNP-sensitive guanylyl cyclase
-
-
CsGC-YO1
Q24LS5
-
GC-A
-
-
GC-A
-
isoform
GC-A
P18293
-
GC-A
-
isoform
GC-A receptor
-
-
GC-B
-
-
GC-B
-
isoform
GC-B
-
isoform
GC-C
-
-
GC-C
Mus musculus BALB/c
-
-
-
GC-C
P55204
-
GC-D
-
-
GC-G
-
-
GC-G
Q6TL19
-
GC1
Mus musculus C57BL/6J
-
-
-
GC2
Mus musculus C57BL/6J
-
-
-
GCC
Q3UWA6
-
GCC
Mus musculus BALB/c
Q3UWA6
-
-
GTP-pyrophosphate lyase
Q8SPV3
-
GTP-pyrophosphate lyase
-
-
guanyl cyclase
-
-
-
-
Guanylate cyclase
-
-
-
-
guanylate cyclase 1
-
-
guanylate cyclase 1
Mus musculus C57BL/6J
-
-
-
guanylate cyclase 2
-
-
guanylate cyclase 2
Mus musculus C57BL/6J
-
-
-
Guanylate cyclase 2D, retinal
-
-
-
-
Guanylate cyclase 2E
-
-
-
-
Guanylate cyclase 2F, retinal
-
-
-
-
guanylate cyclase A
-
-
guanylate cyclase B
-
-
guanylate cyclase C
-
-
guanylate cyclase C
Mus musculus BALB/c
-
-
-
Guanylate cyclase, olfactory
-
-
-
-
guanylyl cyclase
-
-
-
-
guanylyl cyclase
-
-
guanylyl cyclase
-
-
guanylyl cyclase
-
-
guanylyl cyclase A
-
-
guanylyl cyclase A
-
-
guanylyl cyclase B
-
-
guanylyl cyclase C
-
-
guanylyl cyclase C
-
-
guanylyl cyclase C
Q3UWA6
-
guanylyl cyclase C
Mus musculus BALB/c
Q3UWA6
-
-
guanylyl cyclase C
-
-
guanylyl cyclase C receptor
-
-
guanylyl cyclase-A
-
-
guanylyl cyclase-A receptor
-
-
guanylyl cyclase-A receptor
P18293
-
guanylyl cyclase-C
-
-
guanylyl cyclase-C
P55204
-
guanylyl cyclase-D
-
-
guanylyl cyclase-G
-
-
guanylyl cyclase-G
Q6TL19
-
Gyc-88E
-
atypical sGC
Gyc-89Da
-
atypical sGC
Gyc-89Da
Q9VEU6
-
Gyc-89Db
-
atypical sGC
Gyc-89Db
Q9VEU5
-
hSTAR
-
-
-
-
Intestinal guanylate cyclase
-
-
-
-
KSGC
-
-
-
-
membrane bound guanylate cyclase
-
-
membrane bound guanylyl cyclase
-
-
membrane guanylate cyclase
-
-
membrane guanylyl cyclase receptor
-, P16066, P20594, P25092, P51841
-
membrane guanylyl cyclase receptor
-
-
membrane-bound guanylate cyclase
Q8TA72
-
membrane-bound guanylate cyclase
-
-
membrane-bound guanylate cyclase
-
-
membrane-bound guanylate cyclase
-
-
membrane-bound guanylate cyclase
-
-
membrane-bound guanylyl cyclase
-
-
natriuretic peptide-activated guanylate cyclase
P16067, P18910
-
nitric oxid sensitive guanylyl cyclase
-
-
nitric oxide sensitive guanylyl cyclase
-
-
nitric oxide sensitive-guanylyl cyclase
-
-
nitric oxide-sensitive guanylate cyclase
-
-
nitric-oxide-sensitive guanylyl cyclase
-
-
NO receptor
-
alpha1*beta1 isoform of soluble guanylyl cyclase
NO receptor soluble guanylyl cyclase
-
-
NO sensitive guanylyl cyclase
-
-
NO- and haem-independent sGC
-
-
NO- and haem-independent soluble guanylate cyclase
-
-
NO-GC
-
-
NO-GC1
-
-
NO-GC2
-
-
NO-independent, heme-dependent soluble guanylate cyclase
-
-
NO-sensitive GC
-
-
NO-sensitive guanylyl cyclase
B2MVM0
-
NO-sensitive guanylyl cyclase
-
-
NP-GC
-
-
NPR-A
P18910
-
NPR-B
-
-
NPR-B
P16067
-
ONE-GC membrane guanylate cyclase
-
-
particulate GC
-
-
particulate guanylate cyclase
-
-
particulate guanylate cyclase
-
-
particulate guanylyl cyclase
-
-
peptide hormone receptor guanylyl cyclase-C
P55204
-
photoreceptor guanylyl cyclase
-
-
phytosulfokine receptor
-
-
PSK receptor
-
-
PSKR1
-
shows dual guanylate cyclase and kinase catalytic activities in vivo and in vitro
ratGC
-
-
receptor guanylyl cyclase
Q24LS5
-
receptor-type guanylyl cyclase
-, Q2L6K7
-
RET-GC1
Q90WX0, Q90WX1, Q90WX2
-
retGC
-
-
retGC
Q02846
-
RetGC1
-
-
RetGC1
-
isozyme
RetGC2
-
isozyme
retinal guanylate cyclase
-
-
retinal guanylate cyclase
Q02846
-
retinal guanylate cyclase
-
-
retinal guanylyl cyclase
-
-
retinal membrane guanylyl cyclase
-
-
Rod outer segment membrane guanylate cyclase
-
-
-
-
rod outer segment membrane guanylate cyclase type 1
-
-
ROS-GC
-
-
-
-
ROS-GC1
-
-
ROS-GC1
-
-
ROS-GC2
-
-
-
-
sGC
P16068, P19687
-
sGC
-
-
sGC
B2MVM0
-
sGC
B2MVM2
-
sGC
Q81T60
-
sGC
Q8SPV3
-
sGC
P19686
-
sGC
Q02108, Q02153
-
sGC
Q80WY4
-
sGC alpha2 inhibitory isoform
-
-
sGC alpha2 isoform
-
-
sGCalpha1
-
-
sGCalpha2beta1
-
-
sGCbeta1
-
-
sGCbeta1
-
beta1 subunit of soluble guanylyl cyclase
sGCbeta3
B2MVM2
soluble guanylyl cyclase beta-3 subunit
soluble guanylate cyclase
-
-
soluble guanylate cyclase
P16068, P19687
-
soluble guanylate cyclase
-
-
soluble guanylate cyclase
Q5YLC2
-
soluble guanylate cyclase
-
-
soluble guanylate cyclase
-
-
soluble guanylate cyclase
-
-
soluble guanylate cyclase
Q0PY32
-
soluble guanylate cyclase
-
-
soluble guanylate cyclase
-
-
soluble guanylate cyclase
P19686
-
soluble guanylate cyclase
Q02108, Q02153
-
soluble guanylate cyclase
Q80WY4
-
soluble guanylate cyclase
-
-
soluble guanylate cyclase
-
-
soluble guanylate cyclase alpha1
-
-
soluble guanylate cyclase alpha2
-
-
soluble guanylyl cyclase
-
-
soluble guanylyl cyclase
-
-
soluble guanylyl cyclase
-
-
soluble guanylyl cyclase
-
-
soluble guanylyl cyclase
-
-
soluble guanylyl cyclase
-
-
soluble guanylyl cyclase
-
-
soluble guanylyl cyclase
-
-
soluble guanylyl cyclase
Q963L5
-
soluble guanylyl cyclase
-
-
soluble guanylyl cyclase
Q8I7Z5
-
soluble guanylyl cyclase
Leishmania donovani MHOM/IN/1983/AG83
Q8I7Z5
-
-
soluble guanylyl cyclase
B2MVM0
-
soluble guanylyl cyclase
B2MVM2
-
soluble guanylyl cyclase
-
-
soluble guanylyl cyclase
-
-
soluble guanylyl cyclase
-
-
soluble guanylyl cyclase
Q81T60
-
soluble guanylyl cyclase
-
-
soluble guanylyl cyclase
Q8SPV3
-
soluble guanylyl cyclase
-
-
soluble guanylyl cyclase
-
-
soluble guanylyl cyclases
Q9VEU5, Q9VEU6
-
STA
P55204
-
STA receptor
-
-
-
-
mGc
-
-
additional information
-
the enzyme is a member of the family of nucleotide cyclizing enzymes
additional information
Q3UWA6
GC-C is a member of the particulate guanylyl cyclase family
additional information
Mus musculus BALB/c
Q3UWA6
GC-C is a member of the particulate guanylyl cyclase family
-
additional information
-
the enzyme is a member of the family of nucleotide cyclizing enzymes
additional information
Q8SPV3
the enzyme is a member of the family of nucleotide cyclizing enzymes
additional information
-
the enzyme is a member of the family of nucleotide cyclizing enzymes
CAS REGISTRY NUMBER
COMMENTARY
9054-75-5
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
tiger salamander
-
-
Manually annotated by BRENDA team
alpha-subunit
UniProt
Manually annotated by BRENDA team
beta-subunit
UniProt
Manually annotated by BRENDA team
toad
-
-
Manually annotated by BRENDA team
variety Bristol (N2)
-
-
Manually annotated by BRENDA team
variety Bristol (N2)
Q2L6K7
SwissProt
Manually annotated by BRENDA team
gene CsGC-YO1
UniProt
Manually annotated by BRENDA team
mongrel dogs
-
-
Manually annotated by BRENDA team
adult female Hartley guinea pigs
-
-
Manually annotated by BRENDA team
soluble isozyme; gene CYG12 encoding the soluble isozyme
UniProt
Manually annotated by BRENDA team
genes gucy1a3 and gucy2F
-
-
Manually annotated by BRENDA team
RET-GC1; gene gc1
UniProt
Manually annotated by BRENDA team
RET-GC2; gene gc2
UniProt
Manually annotated by BRENDA team
RET-GC3, fragment; gene gc3
UniProt
Manually annotated by BRENDA team
during growth, 20-40% of GC activity is attributed to GCA
-
-
Manually annotated by BRENDA team
GCA, another guanylyl cylase, i.e. soluble guanylyl cyclase (sGC), is present in Dictyostelium; soluble guanylyl cyclase (sGC), another guanylyl cylase, i.e. GCA, is present in Dictyostelium
-
-
Manually annotated by BRENDA team
during growth, 60-80% of GC activity is attributed to sGC
-
-
Manually annotated by BRENDA team
South American opossum, female and male
-
-
Manually annotated by BRENDA team
GC-B; isozyme GC-B
UniProt
Manually annotated by BRENDA team
hypertensive and normotensive Japanese
-
-
Manually annotated by BRENDA team
isozyme GC-A; isozyme GC-A
UniProt
Manually annotated by BRENDA team
isozyme GC-C
UniProt
Manually annotated by BRENDA team
isozyme GC-F; isozyme GC-F
UniProt
Manually annotated by BRENDA team
isozymes GC-D, GC-E, and GC-G
-
-
Manually annotated by BRENDA team
i.e. Ipomoea nil
UniProt
Manually annotated by BRENDA team
MHOM/IN/1983/AG83
SwissProt
Manually annotated by BRENDA team
Leishmania donovani MHOM/IN/1983/AG83
MHOM/IN/1983/AG83
SwissProt
Manually annotated by BRENDA team
-
B2MVM2
UniProt
Manually annotated by BRENDA team
sGC alpha subunit
UniProt
Manually annotated by BRENDA team
129/Sv and C57BL/6 strain hybrids, isozyme GC-A
UniProt
Manually annotated by BRENDA team
Balb/c mice
-
-
Manually annotated by BRENDA team
C57/BL6 mice
-
-
Manually annotated by BRENDA team
C57BL/6 mice
-
-
Manually annotated by BRENDA team
C57BL/6 mice, isozyme sGCalpha1
-
-
Manually annotated by BRENDA team
C57BL/6J background
-
-
Manually annotated by BRENDA team
female and male Swiss/129 mice
-
-
Manually annotated by BRENDA team
GCC; Balb/c mice
UniProt
Manually annotated by BRENDA team
gene Gucy2g encoding isozyme GC-G
UniProt
Manually annotated by BRENDA team
isozyme retGC
-
-
Manually annotated by BRENDA team
isozymes GC-A to GC-G
-
-
Manually annotated by BRENDA team
isozymes NO-GC1 and NO-GC2
-
-
Manually annotated by BRENDA team
male adult C57/Bl6 mice
-
-
Manually annotated by BRENDA team
Swiss/129 mice, isozymes soluble guanylate cyclase alpha1beta and alpha2beta1
-
-
Manually annotated by BRENDA team
Mus musculus BALB/c
Balb/c mice
-
-
Manually annotated by BRENDA team
Mus musculus BALB/c
GCC; Balb/c mice
UniProt
Manually annotated by BRENDA team
Mus musculus C57BL/6J
C57BL/6J background
-
-
Manually annotated by BRENDA team
New Zealand white rabbits
-
-
Manually annotated by BRENDA team
fragment
UniProt
Manually annotated by BRENDA team
Paramecium tetraurelia 51s
strain 51s
-
-
Manually annotated by BRENDA team
adult male Sprague-Dawley rats
-
-
Manually annotated by BRENDA team
beta-subunit; male Wistar-Han rats
UniProt
Manually annotated by BRENDA team
female Sprague-Dawley rats
-
-
Manually annotated by BRENDA team
male sprague-dawley rats
-
-
Manually annotated by BRENDA team
male Wistar rats
-
-
Manually annotated by BRENDA team
male Wistar-Han rats
UniProt
Manually annotated by BRENDA team
NPR-A; adult male Wistar rats
UniProt
Manually annotated by BRENDA team
NPR-B; adult male Wistar rats
UniProt
Manually annotated by BRENDA team
sGC alpha1 subunit; female Wistar rats
UniProt
Manually annotated by BRENDA team
sGC beta1 subunit; female Wistar rats
UniProt
Manually annotated by BRENDA team
Sprague-Dawley rats
-
-
Manually annotated by BRENDA team
Wistar Kyoto and spontaneously hypertensive rats
-
-
Manually annotated by BRENDA team
Wistar, Wistar Kyoto, and spontaneously hypertensive rats, isozyme soluble guanylate cyclase beta1, sGC-beta1
-
-
Manually annotated by BRENDA team
Wistar-Kyoto and spontaneously hypertensive rats
-
-
Manually annotated by BRENDA team
subsp. acutidens
-
-
Manually annotated by BRENDA team
strain B2086
-
-
Manually annotated by BRENDA team
Tetrahymena thermophila B2086
strain B2086
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
-
inhibition of soluble guanylyl cyclase reduces vascular endothelial growth factor-induced angiogenesis and permeability
malfunction
-
guanylyl cyclase-A-deficient mice show cardiac hypertrophy
malfunction
-
guanyl cyclase-C-knockout mice exhibit hyperactivity and attention deficits
physiological function
-
activation of sGC is involved in the nitric oxide-induced increases in outflow facility of the eye
physiological function
-
guanylate cyclase plays a role in migration of A-549 cells
physiological function
-
soluble guanylyl cyclase mediates the angiogenic and permeability-promoting activities of vascular endothelial growth factor. Soluble guanylyl cyclase is a downstream effector of vascular endothelial growth factor-triggered responses
physiological function
-
guanylyl cyclase-A inhibits angiotensin II type 2 receptor-mediated pro-hypertrophic signaling in the heart
physiological function
B2MVM2, -
soluble guanylyl cyclase beta-3 subunit is an oxygen receptor
physiological function
-
nitric oxide sensitive guanylyl cyclase is the major physiological receptor for nitric oxide throughout the cardiovascular and central nervous system
physiological function
-
isozyme RetGC1 is absolutely required for cone function and survival
physiological function
-
soluble guanylate cyclase lowers intracellular Ca2+ concentration in response to nitric oxide, inducing vasodilationand is inhibited by high intracellular Ca2+ concentration, providing a fine balance between signals for vasodilation and vasoconstriction
physiological function
-
soluble guanylate cyclase is an NO-sensing hemoprotein that serves as a nitric oxide receptor in nitric oxide-mediated signaling pathways
physiological function
-
particulate guanylate cyclase is a physiologically relevant source of cGMP in Malpighian tubules
physiological function
-
guanyl cyclase-C activation potentiates the excitatory responses mediated by glutamate and acetylcholine receptors via the activity of GMP-dependent protein kinase
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-O-(N-methylanthraniloyl) guanosine 5'-triphosphate
?
show the reaction diagram
-
-
-
-
?
ATP
adenosine cyclic 3'-5'monophosphate + diphosphate
show the reaction diagram
-
-
-
?
ATP
3',5'-cyclic AMP + diphosphate
show the reaction diagram
-
-
-
-
?
ATP
3',5'-cyclic AMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q02108
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
O75343
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
P16066, P20594, P25092, P51841
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
ir
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-, Q8I7Z5
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q963L5
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
P16067, P18910
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
P16068, P19687
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q02108, Q02153
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-, Q24LS5
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q9VEU5, Q9VEU6
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q8SPV3
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
B2MVM0
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q5YLC2
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q02846
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q90WX0, Q90WX1, Q90WX2
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q6TL19
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
P18293
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q81T60
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q3UWA6
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
P19686, Q80WY4
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-, Q0PY32
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q8TA72
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-, Q2L6K7
-
regulation of intracellular cGMP concentration is essential for normal thermotaxis
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
P16068, P19687
-
cGMP is involved in transmitting the NO activating signals to a variety of downstream effectors such as cyclicnucleotide-gated channels, protein kinases, and phosphodiesterases
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the guanylyl cyclase messenger system is potentially responsive to hormones/neurotranmitters that may control the degree of relaxation in this vascular tissue
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
prefered substrate
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
atrial natriuretic factor receptor guanylate cyclase GC-A is the receptor of the atrial natriuretic factor and the type B natriuretic peptide, overview
cyclic GMP is a second messenger in controlling blood pressure, cardiac vasculature, and fluid secretion
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
cGMP levels in response to relaxant agonists are regulated in gastrointestinal smooth muscle by activation of phosphodiesterase 5 and inhibition of soluble guanylyl cyclase by c-Src-dependent phosphorylation in a feedback mechanism via the cGMP-dependent protein kinase, mechanism for attenuation of the NO/sGC/cGMP signal by Gi-coupled contractile agonists, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
desensitization of the soluble guanylyl cyclase/cGMP pathway by lipopolysaccharide in rat isolated pulmonary artery but not aorta, leading to muscle relaxation
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
GC-A acts as receptor for atrial natriuretic peptide that regulates arterial blood pressure and volume, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
GTP is a chemorepellent in Tetrahymena thermophila that stimulates cell division as well as ciliary reversal. Protein kinase C activy is not required for GTP signaling
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
guanylyl cyclase receptors synthesize the second-messenger cyclic GMP
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
P16066, P20594, P25092, P51841
guanylyl cyclase receptors synthesize the second-messenger cyclic GMP
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
P55204
guanylyl cyclase-C is a peptide hormone receptor
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
in the heart exists a coupled multienzymatic system for selective regulation of indirect, sGC-dependent versus direct, sGC-independent NO- and redox-related modulation of voltage-gated ion channel function in different myocyte types, mechanisms, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
isozyme GC-A is a common receptor for atrial and brain natriuretic peptide
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q9VEU5, Q9VEU6
isozyme Gyc-89Da in neurons is necessary early in adult development to prevent eclosion, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q9VEU5, Q9VEU6
isozyme Gyc-89Db in neurons is necessary early in adult development to prevent eclosion, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
mechanism of NO stimulation of cGMP production in airway hyperreactivity pathogenesis evoked by toluene inhalation, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q6TL19
membrane forms of guanylyl cyclase serve as cell-surface receptors that synthesize the second messenger cGMP, which mediates diverse cellular processes. GC-G plays a role in mediating injury, GC-G may act as an early signaling molecule that promotes apoptotic and inflammatory responses in I/R-induced acute renal injury
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
nitric oxide and carbon monoxide are modulators of neurotransmission in cardiac ganglia and in neural control of the adult human heart
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
NO is a major signaling molecule in the gastrointestinal tract, and released NO inhibits muscular contraction. The actions of NO are mediated by stimulation of NO-sensitive sGC and a subsequent increase in cGMP concentration
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
NO receptor isozymes NO-GC1 and NO-GC2 are required for long term potentiation in smooth muscle relaxation, they mediate vasorelaxation and platelet-inhibition of nitric oxide being the only NO receptor of the signalling pathway
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
NO-GC plays a role in neuropathic pain. cGMP produced by NO-GC may activate signaling pathways different from cGMP-dependent protein kinase I, cGKI, during spinal nociceptive processing, whereas cGKI can be activated by natriuretic peptide receptor-B dependent cGMP production, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
physiological function of the enzyme in diabetes and obesity, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
role of sGCalpha1beta1 in nitrergic regulation of jejunal smooth muscle activity in male and female mice, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q02108, Q02153
role of soluble guanylate cyclase during the inflammatory phase of postoperative illius, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
sGC activity plays an important role in a variety of aspects of the cardiovascular system, regulatory mechanisms, overview. sGC has an anti-proliferative effect on smooth muscle cells
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q8SPV3
sGC activity plays an important role in a variety of aspects of the cardiovascular system, regulatory mechanisms, overview. sGC has an anti-proliferative effect on smooth muscle cells
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
sGC contributes to the pathology of pulmonary arterial hypertension, its stimulation reverses right heart hypertrophy and structural lung vascular remodelling, hemodynamics and vascular remodelling in a mouse lung model, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
sGC contributes to the pathology of pulmonary arterial hypertension, its stimulation reverses right heart hypertrophy and structural lung vascular remodelling, hemodynamics and vascular remodelling in a rat lung model, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
sGC contributes to the pathology of pulmonary arterial hypertension, its stimulation reverses right heart hypertrophy and structural lung vascular remodelling, hemodynamics and vascular remodelling in lung explants, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
sGC is a receptor for nitric oxide generating cGMP
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
sGC produces cGMP, a second messenger required for normal vascular smooth muscle cell, VSMC, relaxation. Activating NADPH oxidase in bovine aortic VSMC increases ROS levels and induces oxidative posttranslational modification of Cys122, a beta1-subunit cysteinyl residue of the guanylate cyclase leading to its inhibition
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
P19686, Q80WY4
soluble guanylate cyclase is a key element in NO signaling, activation of sGC is an important mechanism of vascular collapse during septic shock
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
P19686, Q80WY4
soluble guanylate cyclase is a key element in NO signaling, activation of sGC is an important mechanism of vascular collapse duringseptic shock
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
soluble guanylate cyclase is the mammalian receptor for nitric oxide
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
soluble guanylyl cyclase contributes to ventilator-induced lung injury in mice, enzyme inhibition in lung increases the filtration coefficient, regulation, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
soluble guanylyl cyclase is a key protein in the NO/cGMP signaling pathway
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
soluble guanylyl cyclase is the principal receptor for NO and plays a ubiquitous role in regulating cellular function, sGC governs smooth muscle tone and growth, vascular permeability, leukocyte flux, and platelet aggregation. Aberrant NO-sGC signaling is linked to diseases including hypertension, atherosclerosis, and stroke
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the cGMP-dependent protein kinase negatively regulates sGC activity, phosphorylation at Ser64 desensitizes sGC and dampens NO signaling, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-, Q24LS5
the enzyme acts as a receptor for the molt-inhibiting hormone
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the enzyme and cGMP-pathway are involved in NO-mediated cell migration
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the enzyme has a role in neuronal degeneration
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the enzyme is important in regulation of cardiaovascular functions and vision in humans, allosteric regulation, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the enzyme is involved in regualtion of artery contaction, enzyme activation by NO leads to increased pulmonary artery relaxation
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the enzyme is involved in vasorelaxation, NO acts as a signalling molecule regulating sGC expression in a negative feedback loop, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
P18293
the homodimeric transmembrane guanylyl cyclase-A receptor produces cytoplasmic cyclic GMP from GTP on binding its extracellular ligands, atrial and B-type natriuretic peptides, which modulate blood pressure and volume through the stimulation of cyclic GMP production by their guanylyl cyclase-A receptor, overview. Alternative splicing can regulate endogenous ANP/GC-A signaling, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the membrane guanylate cyclase is part of a transduction machinery involving the guanylate cyclae, the guanylate cyclase activating protein type 1, S100B, and neurocalcin delta, mechanism, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the NO-independent, heme-dependent soluble guanylate cyclase acts as an NO receptor
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-, Q24LS5
the receptor guanylyl cyclase acts as a molt-inhibiting hormone receptor and is a functional link to ecdysteroidogenesis, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the receptor membrane-bound guanylate cyclase in the sperm tail triggers sperm chemotaxis, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q8TA72
the receptor membrane-bound guanylate cyclase in the sperm tail triggers sperm chemotaxis, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the receptor membrane-bound guanylate cyclase in the sperm tail triggers sperm chemotaxis, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the regulation of arterial vasodilatory and cardiac beta-adrenergic reserve by phosphodiesterase type 5-I targets cGMP from soluble GC stimulation
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the retina-specific guanylyl cyclases, retGC1 and retGC2 support synthesis of cGMP in photoreceptors
cyclic GMP serves as the second messenger in visual transduction, linking photon absorption by rhodopsin to the activity of ion channels
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the retinal isozyme retGC-1 influences transducin movement, not through its cyclase activity, but through direct interaction with Galphat protein, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q02846
the retinal isozyme retGC-1 influences transducin movement, not through its cyclase activity, but through direct interaction with Galphat protein, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the soluble guanylate cyclase isoforms alpha1beta1 and alpha2beta1 are involved in the relaxation of distal colon by exogenous NO and by NANC nerve stimulation, mechanism, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
B2MVM0
the soluble guanylyl cyclase is the major receptor for NO and contributes to prolonged depolarization of the membrane potential in cerebral giant cells, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
von Willebrand factor/ristocetin-mediated activation of the sGC/cGMP signaling pathway may contribute to feedback platelet inhibition, regulation mechanisms, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
P55204
guanylin-GC-C interaction, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Leishmania donovani MHOM/IN/1983/AG83
Q8I7Z5
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Mus musculus BALB/c
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Mus musculus BALB/c
Q3UWA6
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Paramecium tetraurelia 51s
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Tetrahymena thermophila B2086
-
-, GTP is a chemorepellent in Tetrahymena thermophila that stimulates cell division as well as ciliary reversal. Protein kinase C activy is not required for GTP signaling
-
-
?
GTP
?
show the reaction diagram
-
-
-
-
-
GTP
?
show the reaction diagram
-
MgGTP2-
-
-
-
GTP
3',5'-cyclic-GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic-GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic-GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic-GMP + diphosphate
show the reaction diagram
P20595
-
-
-
?
GTP
3',5'-cyclic-GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic-GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic-GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic-GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic-GMP + diphosphate
show the reaction diagram
B2MVM2, -
-
-
-
?
GTP
3',5'-cyclic-GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic-GMP + diphosphate
show the reaction diagram
Q7CH76
class IV adenylyl cyclase also functions as guanylyl cyclase with about 20% efficiency
-
-
?
GTP
3',5'-cyclic-GMP + diphosphate
show the reaction diagram
-
guanylate cyclase activity is nitric oxide-dependent. The recombinant enzyme shows substrate specificity for GTP rather than ATP
-
-
?
GTP
3',5'-cyclic-GMP + diphosphate
show the reaction diagram
-
isozyme RetGC1 contributes 23-28% to the maximal cGMP synthesis rate in mouse rod outer segments. The activity of isozyme RetGC2 is about 5times lower than isozyme RetGC1
-
-
?
GTPgammaS
?
show the reaction diagram
-
-
-
-
?
guanosine 5'-beta,gamma-methylene triphosphate
?
show the reaction diagram
-
-
-
-
?
guanosine-5'-[alpha,beta-methylene]triphosphate
?
show the reaction diagram
-
-
-
-
?
guanyl-(beta,gamma-methylene)-diphosphate
?
show the reaction diagram
-
-
-
?
UTP
3',5'-cyclic UMP + diphosphate
show the reaction diagram
-
-
-
-
?
guanyl-imidodiphosphate
?
show the reaction diagram
-
-
-
?
additional information
?
-
-
ATP is no substrate
-
-
-
additional information
?
-
-
soluble guanylyl cyclase sGC provides the major guanylyl cyclase activity in Dictyostelium, contributing about 90% of the chemoattractant-induced cGMP response
-
-
-
additional information
?
-
-
2'-deoxy-3'-GMP and cGMP exhibit no detectable affinity for the enzyme
-
-
-
additional information
?
-
-
GMPNH-P, GMPCH-P and N-methylanthraniloyl 2'-GTP are poor substrates
-
-
?
additional information
?
-
-
no considerable activity with ATP
-
-
-
additional information
?
-
-
structure-function relationship, overview
-
-
-
additional information
?
-
-
association of CT dinucleotide repeat polymorphism in the 5'-flanking region of the guanylyl cyclase A gene with essential hypertension in the Japanese, overview
-
-
-
additional information
?
-
-
ATP-dependent mode of ANF-RGC signal transduction mechanism, overview
-
-
-
additional information
?
-
-
guanylate cyclase inhibitor LY-83583 completely blocks high frequency stimuli at 20 Hz/20 s-induced ganglionic long-term potentiation, gLTP, in superior cervical ganglia isolated from control rats, overview
-
-
-
additional information
?
-
-
guanylyl cyclase and protein kinase G mediate nitric oxide suppression of 5-lipoxygenase metabolism in rat alveolar macrophages, regulation mechanisms, overview
-
-
-
additional information
?
-
-
heme oxygenase-1 induction depletes heme and attenuates pulmonary artery relaxation and guanylate cyclase activation by nitric oxide
-
-
-
additional information
?
-
-
hypertension is related to reduced cGMP levels, spontaneously hypertensive rats shows reduced sGC levels, overview, sGC role in vascular system diseases and failures, overview
-
-
-
additional information
?
-
-
in vivo mechanism of GTP avoidance, overview
-
-
-
additional information
?
-
-
inhibition of non-small cell lung cancer cell migration by grape seed proanthocyanidins is mediated through the inhibition of nitric oxide, guanylate cyclase, and ERK1/2, overview
-
-
-
additional information
?
-
-
inhibition of soluble guanylate cyclase abolishes the potentiating effect of Mn2+ on MAP kinase phosphorylation, NF-kappaB activation, and production of NO
-
-
-
additional information
?
-
-, P16066, P20594, P25092, P51841
isozyme GC-A mediates the endocrine effects of atrial and B-type natriuretic peptides regulating arterial blood pressure and volume homeostasis and also local antihypertrophic and antifibrotic actions in the heart, overview
-
-
-
additional information
?
-
-
isozyme GC-A mediates the endocrine effects of atrial and B-type natriuretic peptides regulating arterial blood pressure and volume homeostasis and also local antihypertrophic and antifibrotic actions in the heart. Isozyme GC-B, the specific receptor for C-type natriuretic peptide, has a critical role in endochondral ossification. Isozyme GC-C mediates the effects of guanylin and uroguanylin on intestinal electrolyte and water transport and epithelial cell growth and differentiation. Isozymes GC-E and GC-F are colocalized within the same photoreceptor cells of the retina and have an important role in phototransduction. Isozyme GC-D has chemosensory functions in the olfactory neuroepithelium
-
-
-
additional information
?
-
-, P16066, P20594, P25092, P51841
isozyme GC-B, the specific receptor for C-type natriuretic peptide, has a critical role in endochondral ossification, overview
-
-
-
additional information
?
-
-, P16066, P20594, P25092, P51841
isozyme GC-C mediates the effects of guanylin and uroguanylin on intestinal electrolyte and water transport and epithelial cell growth and differentiation, overview
-
-
-
additional information
?
-
-, P16066, P20594, P25092, P51841
isozymes GC-E and GC-F are colocalized within the same photoreceptor cells of the retina and have an important role in phototransduction, overview
-
-
-
additional information
?
-
-, P16066, P20594, P25092, P51841
Isozymes GC-E and GC-F are colocalized within the same photoreceptor cells of the retina and have an important role in phototransduction. Isozymes GC-D and GC-G appear to be pseudogenes in the human, role of alterations of these ligand/receptor systems in human diseases, overview
-
-
-
additional information
?
-
-
modeling of the intracellular signal transduction for CO2 detection by GC-D+ neurons and the molecular mechanism of bicarbonate sensing by GC-D, overview
-
-
-
additional information
?
-
-
sGC mediates the NO-induced aqueous humor secretion and increased outflow facility from the eye
-
-
-
additional information
?
-
-
sGC role in vascular system diseases and failures, overview
-
-
-
additional information
?
-
Q8SPV3
sGC role in vascular system diseases and failures, overview
-
-
-
additional information
?
-
-
soluble guanylyl cyclase alpha1/alpha2 subunits are involved in the relaxant effect of CO and CORM-2, i.e. CO-releasing molecule-2, on enteric smooth muscle, overview
-
-
-
additional information
?
-
-
splicing is a method of sGC regulation, direct regulation of guanylyl cyclase by dominant-negative splice variants. alpha1 Soluble guanylyl cyclase splice forms act as regulators of human sGC activity, overview
-
-
-
additional information
?
-
-
sustained soluble guanylate cyclase stimulation offsets nitric-oxide synthase inhibition to restore acute cardiac modulation by sildenafil, overview
-
-
-
additional information
?
-
-
the atrial natriuretic peptide/GC-A/cGMP-dependent protein kinase I system stimulates the phosphorylation of VASP in cultured microvascular endothelial cells
-
-
-
additional information
?
-
-
the NO/sGC/cGMP signaling cascade is not critically involved in ODQ-induced neurite remodeling, overview
-
-
-
additional information
?
-
-
molecular basis of substrate specificity, overview
-
-
-
additional information
?
-
-
structure and functions of membrane guanylate cyclase isozymes, overview
-
-
-
additional information
?
-
Q5YLC2
structure-function relationship, mechanism, modelling, overview
-
-
-
additional information
?
-
-
the enzyme binds the human rod Galphat protein without altering cyclase activity, interactions with ligands, overview
-
-
-
additional information
?
-
-
the enzyme binds the human rod Galphat protein without altering cyclase activity, overview
-
-
-
additional information
?
-
-
under anaerobic conditions nitric oxide (NO) binds to the protein in its resting state (NOGC1-Fe3+) and NO still remains bound to the protein in its reduced state (NOGC1-Fe2+). NO binds to the ferri-heme (resting state) and ferroheme (reduced state) while O2 binds preferentially to ferro-heme. NO has a higher affinity for the enzyme heme site than O2
-
-
-
additional information
?
-
Tetrahymena thermophila B2086
-
in vivo mechanism of GTP avoidance, overview
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q02108
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
O75343
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
P16067, P18910
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q5YLC2
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q90WX0, Q90WX1, Q90WX2
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q81T60
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q3UWA6
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-, Q0PY32
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
P16068, P19687
-
cGMP is involved in transmitting the NO activating signals to a variety of downstream effectors such as cyclicnucleotide-gated channels, protein kinases, and phosphodiesterases
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the guanylyl cyclase messenger system is potentially responsive to hormones/neurotranmitters that may control the degree of relaxation in this vascular tissue
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
atrial natriuretic factor receptor guanylate cyclase GC-A is the receptor of the atrial natriuretic factor and the type B natriuretic peptide, overview
cyclic GMP is a second messenger in controlling blood pressure, cardiac vasculature, and fluid secretion
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
cGMP levels in response to relaxant agonists are regulated in gastrointestinal smooth muscle by activation of phosphodiesterase 5 and inhibition of soluble guanylyl cyclase by c-Src-dependent phosphorylation in a feedback mechanism via the cGMP-dependent protein kinase, mechanism for attenuation of the NO/sGC/cGMP signal by Gi-coupled contractile agonists, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
desensitization of the soluble guanylyl cyclase/cGMP pathway by lipopolysaccharide in rat isolated pulmonary artery but not aorta, leading to muscle relaxation
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
GC-A acts as receptor for atrial natriuretic peptide that regulates arterial blood pressure and volume, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
GTP is a chemorepellent in Tetrahymena thermophila that stimulates cell division as well as ciliary reversal. Protein kinase C activy is not required for GTP signaling
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
guanylyl cyclase receptors synthesize the second-messenger cyclic GMP
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
P16066, P20594, P25092, P51841
guanylyl cyclase receptors synthesize the second-messenger cyclic GMP
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
P55204
guanylyl cyclase-C is a peptide hormone receptor
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
in the heart exists a coupled multienzymatic system for selective regulation of indirect, sGC-dependent versus direct, sGC-independent NO- and redox-related modulation of voltage-gated ion channel function in different myocyte types, mechanisms, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
isozyme GC-A is a common receptor for atrial and brain natriuretic peptide
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q9VEU5, Q9VEU6
isozyme Gyc-89Da in neurons is necessary early in adult development to prevent eclosion, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q9VEU5, Q9VEU6
isozyme Gyc-89Db in neurons is necessary early in adult development to prevent eclosion, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
mechanism of NO stimulation of cGMP production in airway hyperreactivity pathogenesis evoked by toluene inhalation, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q6TL19
membrane forms of guanylyl cyclase serve as cell-surface receptors that synthesize the second messenger cGMP, which mediates diverse cellular processes. GC-G plays a role in mediating injury, GC-G may act as an early signaling molecule that promotes apoptotic and inflammatory responses in I/R-induced acute renal injury
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
nitric oxide and carbon monoxide are modulators of neurotransmission in cardiac ganglia and in neural control of the adult human heart
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
NO is a major signaling molecule in the gastrointestinal tract, and released NO inhibits muscular contraction. The actions of NO are mediated by stimulation of NO-sensitive sGC and a subsequent increase in cGMP concentration
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
NO receptor isozymes NO-GC1 and NO-GC2 are required for long term potentiation in smooth muscle relaxation, they mediate vasorelaxation and platelet-inhibition of nitric oxide being the only NO receptor of the signalling pathway
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
NO-GC plays a role in neuropathic pain. cGMP produced by NO-GC may activate signaling pathways different from cGMP-dependent protein kinase I, cGKI, during spinal nociceptive processing, whereas cGKI can be activated by natriuretic peptide receptor-B dependent cGMP production, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
physiological function of the enzyme in diabetes and obesity, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
role of sGCalpha1beta1 in nitrergic regulation of jejunal smooth muscle activity in male and female mice, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q02108, Q02153
role of soluble guanylate cyclase during the inflammatory phase of postoperative illius, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
sGC activity plays an important role in a variety of aspects of the cardiovascular system, regulatory mechanisms, overview. sGC has an anti-proliferative effect on smooth muscle cells
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q8SPV3
sGC activity plays an important role in a variety of aspects of the cardiovascular system, regulatory mechanisms, overview. sGC has an anti-proliferative effect on smooth muscle cells
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
sGC contributes to the pathology of pulmonary arterial hypertension, its stimulation reverses right heart hypertrophy and structural lung vascular remodelling, hemodynamics and vascular remodelling in a mouse lung model, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
sGC contributes to the pathology of pulmonary arterial hypertension, its stimulation reverses right heart hypertrophy and structural lung vascular remodelling, hemodynamics and vascular remodelling in a rat lung model, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
sGC contributes to the pathology of pulmonary arterial hypertension, its stimulation reverses right heart hypertrophy and structural lung vascular remodelling, hemodynamics and vascular remodelling in lung explants, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
sGC is a receptor for nitric oxide generating cGMP
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
sGC produces cGMP, a second messenger required for normal vascular smooth muscle cell, VSMC, relaxation. Activating NADPH oxidase in bovine aortic VSMC increases ROS levels and induces oxidative posttranslational modification of Cys122, a beta1-subunit cysteinyl residue of the guanylate cyclase leading to its inhibition
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
P19686, Q80WY4
soluble guanylate cyclase is a key element in NO signaling, activation of sGC is an important mechanism of vascular collapse during septic shock
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
P19686, Q80WY4
soluble guanylate cyclase is a key element in NO signaling, activation of sGC is an important mechanism of vascular collapse duringseptic shock
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
soluble guanylate cyclase is the mammalian receptor for nitric oxide
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
soluble guanylyl cyclase contributes to ventilator-induced lung injury in mice, enzyme inhibition in lung increases the filtration coefficient, regulation, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
soluble guanylyl cyclase is a key protein in the NO/cGMP signaling pathway
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
soluble guanylyl cyclase is the principal receptor for NO and plays a ubiquitous role in regulating cellular function, sGC governs smooth muscle tone and growth, vascular permeability, leukocyte flux, and platelet aggregation. Aberrant NO-sGC signaling is linked to diseases including hypertension, atherosclerosis, and stroke
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the cGMP-dependent protein kinase negatively regulates sGC activity, phosphorylation at Ser64 desensitizes sGC and dampens NO signaling, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-, Q24LS5
the enzyme acts as a receptor for the molt-inhibiting hormone
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the enzyme and cGMP-pathway are involved in NO-mediated cell migration
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the enzyme has a role in neuronal degeneration
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the enzyme is important in regulation of cardiaovascular functions and vision in humans, allosteric regulation, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the enzyme is involved in regualtion of artery contaction, enzyme activation by NO leads to increased pulmonary artery relaxation
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the enzyme is involved in vasorelaxation, NO acts as a signalling molecule regulating sGC expression in a negative feedback loop, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
P18293
the homodimeric transmembrane guanylyl cyclase-A receptor produces cytoplasmic cyclic GMP from GTP on binding its extracellular ligands, atrial and B-type natriuretic peptides, which modulate blood pressure and volume through the stimulation of cyclic GMP production by their guanylyl cyclase-A receptor, overview. Alternative splicing can regulate endogenous ANP/GC-A signaling, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the membrane guanylate cyclase is part of a transduction machinery involving the guanylate cyclae, the guanylate cyclase activating protein type 1, S100B, and neurocalcin delta, mechanism, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the NO-independent, heme-dependent soluble guanylate cyclase acts as an NO receptor
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-, Q24LS5
the receptor guanylyl cyclase acts as a molt-inhibiting hormone receptor and is a functional link to ecdysteroidogenesis, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the receptor membrane-bound guanylate cyclase in the sperm tail triggers sperm chemotaxis, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q8TA72
the receptor membrane-bound guanylate cyclase in the sperm tail triggers sperm chemotaxis, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the receptor membrane-bound guanylate cyclase in the sperm tail triggers sperm chemotaxis, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the regulation of arterial vasodilatory and cardiac beta-adrenergic reserve by phosphodiesterase type 5-I targets cGMP from soluble GC stimulation
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the retina-specific guanylyl cyclases, retGC1 and retGC2 support synthesis of cGMP in photoreceptors
cyclic GMP serves as the second messenger in visual transduction, linking photon absorption by rhodopsin to the activity of ion channels
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the retinal isozyme retGC-1 influences transducin movement, not through its cyclase activity, but through direct interaction with Galphat protein, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Q02846
the retinal isozyme retGC-1 influences transducin movement, not through its cyclase activity, but through direct interaction with Galphat protein, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
the soluble guanylate cyclase isoforms alpha1beta1 and alpha2beta1 are involved in the relaxation of distal colon by exogenous NO and by NANC nerve stimulation, mechanism, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
B2MVM0
the soluble guanylyl cyclase is the major receptor for NO and contributes to prolonged depolarization of the membrane potential in cerebral giant cells, overview
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
-
von Willebrand factor/ristocetin-mediated activation of the sGC/cGMP signaling pathway may contribute to feedback platelet inhibition, regulation mechanisms, overview
-
-
?
GTP
?
show the reaction diagram
-
-
-
-
-
GTP
?
show the reaction diagram
-
MgGTP2-
-
-
-
GTP
3',5'-cyclic-GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic-GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic-GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic-GMP + diphosphate
show the reaction diagram
P20595
-
-
-
?
GTP
3',5'-cyclic-GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic-GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic-GMP + diphosphate
show the reaction diagram
B2MVM2, -
-
-
-
?
GTP
3',5'-cyclic-GMP + diphosphate
show the reaction diagram
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Mus musculus BALB/c
-
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Mus musculus BALB/c
Q3UWA6
-
-
-
?
GTP
3',5'-cyclic GMP + diphosphate
show the reaction diagram
Tetrahymena thermophila B2086
-
GTP is a chemorepellent in Tetrahymena thermophila that stimulates cell division as well as ciliary reversal. Protein kinase C activy is not required for GTP signaling
-
-
?
additional information
?
-
-
soluble guanylyl cyclase sGC provides the major guanylyl cyclase activity in Dictyostelium, contributing about 90% of the chemoattractant-induced cGMP response
-
-
-
additional information
?
-
-
association of CT dinucleotide repeat polymorphism in the 5'-flanking region of the guanylyl cyclase A gene with essential hypertension in the Japanese, overview
-
-
-
additional information
?
-
-
ATP-dependent mode of ANF-RGC signal transduction mechanism, overview
-
-
-
additional information
?
-
-
guanylate cyclase inhibitor LY-83583 completely blocks high frequency stimuli at 20 Hz/20 s-induced ganglionic long-term potentiation, gLTP, in superior cervical ganglia isolated from control rats, overview
-
-
-
additional information
?
-
-
guanylyl cyclase and protein kinase G mediate nitric oxide suppression of 5-lipoxygenase metabolism in rat alveolar macrophages, regulation mechanisms, overview
-
-
-
additional information
?
-
-
heme oxygenase-1 induction depletes heme and attenuates pulmonary artery relaxation and guanylate cyclase activation by nitric oxide
-
-
-
additional information
?
-
-
hypertension is related to reduced cGMP levels, spontaneously hypertensive rats shows reduced sGC levels, overview, sGC role in vascular system diseases and failures, overview
-
-
-
additional information
?
-
-
in vivo mechanism of GTP avoidance, overview
-
-
-
additional information
?
-
-
inhibition of non-small cell lung cancer cell migration by grape seed proanthocyanidins is mediated through the inhibition of nitric oxide, guanylate cyclase, and ERK1/2, overview
-
-
-
additional information
?
-
-
inhibition of soluble guanylate cyclase abolishes the potentiating effect of Mn2+ on MAP kinase phosphorylation, NF-kappaB activation, and production of NO
-
-
-
additional information
?
-
-, P16066, P20594, P25092, P51841
isozyme GC-A mediates the endocrine effects of atrial and B-type natriuretic peptides regulating arterial blood pressure and volume homeostasis and also local antihypertrophic and antifibrotic actions in the heart, overview
-
-
-
additional information
?
-
-
isozyme GC-A mediates the endocrine effects of atrial and B-type natriuretic peptides regulating arterial blood pressure and volume homeostasis and also local antihypertrophic and antifibrotic actions in the heart. Isozyme GC-B, the specific receptor for C-type natriuretic peptide, has a critical role in endochondral ossification. Isozyme GC-C mediates the effects of guanylin and uroguanylin on intestinal electrolyte and water transport and epithelial cell growth and differentiation. Isozymes GC-E and GC-F are colocalized within the same photoreceptor cells of the retina and have an important role in phototransduction. Isozyme GC-D has chemosensory functions in the olfactory neuroepithelium
-
-
-
additional information
?
-
-, P16066, P20594, P25092, P51841
isozyme GC-B, the specific receptor for C-type natriuretic peptide, has a critical role in endochondral ossification, overview
-
-
-
additional information
?
-
-, P16066, P20594, P25092, P51841
isozyme GC-C mediates the effects of guanylin and uroguanylin on intestinal electrolyte and water transport and epithelial cell growth and differentiation, overview
-
-
-
additional information
?
-
-, P16066, P20594, P25092, P51841
isozymes GC-E and GC-F are colocalized within the same photoreceptor cells of the retina and have an important role in phototransduction, overview
-
-
-
additional information
?
-
-, P16066, P20594, P25092, P51841
Isozymes GC-E and GC-F are colocalized within the same photoreceptor cells of the retina and have an important role in phototransduction. Isozymes GC-D and GC-G appear to be pseudogenes in the human, role of alterations of these ligand/receptor systems in human diseases, overview
-
-
-
additional information
?
-
-
modeling of the intracellular signal transduction for CO2 detection by GC-D+ neurons and the molecular mechanism of bicarbonate sensing by GC-D, overview
-
-
-
additional information
?
-
-
sGC mediates the NO-induced aqueous humor secretion and increased outflow facility from the eye
-
-
-
additional information
?
-
-
sGC role in vascular system diseases and failures, overview
-
-
-
additional information
?
-
Q8SPV3
sGC role in vascular system diseases and failures, overview
-
-
-
additional information
?
-
-
soluble guanylyl cyclase alpha1/alpha2 subunits are involved in the relaxant effect of CO and CORM-2, i.e. CO-releasing molecule-2, on enteric smooth muscle, overview
-
-
-
additional information
?
-
-
splicing is a method of sGC regulation, direct regulation of guanylyl cyclase by dominant-negative splice variants. alpha1 Soluble guanylyl cyclase splice forms act as regulators of human sGC activity, overview
-
-
-
additional information
?
-
-
sustained soluble guanylate cyclase stimulation offsets nitric-oxide synthase inhibition to restore acute cardiac modulation by sildenafil, overview
-
-
-
additional information
?
-
-
the atrial natriuretic peptide/GC-A/cGMP-dependent protein kinase I system stimulates the phosphorylation of VASP in cultured microvascular endothelial cells
-
-
-
additional information
?
-
-
the NO/sGC/cGMP signaling cascade is not critically involved in ODQ-induced neurite remodeling, overview
-
-
-
additional information
?
-
Tetrahymena thermophila B2086
-
in vivo mechanism of GTP avoidance, overview
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
ATP
-
activates at lower concentrations ,0.1 mM
ATP
-
ATP activates guanylyl cyclase isoforms GC-A and GC-B. 0.01 mM Go6976 increases the potency of ATP for isoform GC-B 4fold
Calmodulin
-
activation in presence of Ca2+
heme
-
heme domain is localized to the N-terminal 194 residues of the beta1 subunit. Characterization of the minimum functional ligand-binding heme domain derived from soluble guanylate cyclase
heme
-
heme depletion leads to inhibition of enzyme activation by NO
heme
-
sGC is a hemoprotein
heme
Q5YLC2
in soluble guanylate cyclases
heme
Q8SPV3
-
heme
-
NO-independent, heme-dependent soluble guanylate cyclase
heme
Q81T60
hallmark of sGC activation by NO is the release of beta1 His105 from the heme, leading to allosteric stimulation of cyclase activity
heme
P19686, Q80WY4
;
heme
P16068, P19687
one heme per heterodimer; one heme per heterodimer
heme
-
one heme per heterodimer
heme
-
the enzyme possesses a heme-containing beta subunit
heme
-
the weak Fe2+-proximal His bond is a key determinant for the low O2 affinity of the heme moiety of soluble guanylate cyclase
tin(IV) protophorphyrin IX (2+)
-
activation
zinc (II) protophorphyrin IX
-
activation at nM concentrations
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
activation at 0.05 mM; activation of soluble enzyme
Ca2+
-
activation of soluble enzyme
Ca2+
-
activation of soluble enzyme
Ca2+
-
Ca2+-dependent negative feedback control mediated by guanylate-activating proteins
Ca2+
-, Q8I7Z5
dose-dependent stimulation
Ca2+
-
-
Ca2+
Q90WX0, Q90WX1, Q90WX2
-
Ca2+
-
modulates the membrane guanylate cyclase transduction machinery by both inhibiting and stimulating it involving the guanylate cyclae, the guanylate cyclase activating protein type 1, S100B, and neurocalcin delta, mechanism, overview
Ca2+
-
the two neuronal calcium sensors, GCAP1 and GCAP2, confer Ca2+ sensitivity to guanylyl cyclase activity
Ca2+
-
activates, is required for activation of the retinal enzyme by cone-specific calcium sensor guanylate cyclase activating protein 4
CO
-
carbon monoxide is a modulator of neurotransmission in cardiac ganglia and in neural control of the adult human heart
CO
Q81T60
competitive binding, off rates are reduced by YC-1, but YC-1 stimulation of CO binding is unaltered for the heme-intact portion of both mutated proteins, CO release from msGC-NT1-CO is measured in a stopped-flow device by replacement with NO, which binds more quickly and tightly to the enzyme, overview
Fe2+
-
Gyc-88E(1-597) is a Fe(II)-O2 complex
Fe2+
-
ligands of the form XO can bind to the heme Fe in either a linear or bent configuration
Fe2+
-
beta2 homodimer forms a five-coordinate Fe(II)-NO-complex
Fe2+
-
-
Fe2+
-
L1 H-NOX forms a temperature-independent 5-coordinate FeII-NO complex, L2 H-NOX forms a temperature-dependent mixture of 5- and 6-coordinate FeII-NO complexes
Fe2+
-
forms a temperature-dependent mixture of 5- and 6-coordinate FeII-NO complexes
Fe2+
-
part of the heme cofactor, analysis of the Fe-NO conformation, overview
Mg2+
-
one Mg2+ is primarily associated with the enzyme and the second is directly bound to the alpha, beta and gamma phosphates of GTP
Mg2+
-
predominantly active with Mg2+/GTP
Mg2+
-
maximal activity at 1-2 mM, 5fold lower activity than with Mn2+
Mg2+
-
physiological cofactor. Like the full-length enzyme, the alphacatbetacat complex is more active in presence of Mn2+ as compared to the physiological cofactor
Mg2+
-
guanylyl cyclase GCA is about 5fold more active with optimal Mg2+ concentrations than with optimal Mn2+ concentrations; soluble guanylyl cyclase sGC is about 6fold more active with 2 mM Mn2+ than with 2 mM Mg2+
Mg2+
-, Q8I7Z5
-
Mg2+
-
activates up to 2 mM, in the presence of small amounts of Mn2+ activation increases dramatically
Mg2+
Q90WX0, Q90WX1, Q90WX2
-
Mg2+
Q81T60
-
Mg2+
-, Q0PY32
sGC requires a cation cofactor
Mg2+
P16068, P19687
required; required
Mg2+
-
dependent on
Mg2+
-
required
Mn2+
-
supports only 30% of Mg2+-dependent activity
Mn2+
-
maximal activity at 1-2 mM
Mn2+
-
like the full-length enzyme, the alphacatbetacat complex is more active in presence of Mn2+ as compared to the physiological cofactor
Mn2+
-
activates
Mn2+
-
guanylyl cyclase GCA is about 5fold more active with optimal Mg2+ concentrations than with optimal Mn2+ concentrations; soluble guanylyl cyclase sGC is about 6fold more active with 2 mM Mn2+ than with 2 mM Mg2+
Mn2+
-, Q8I7Z5
the recombinant enzyme shows substantially low level of activity when Mg2+ is replaced with Mn2+
Mn2+
Q5YLC2
most effective, Mg2+ activates to 1% of the activity with Mn2+
Mn2+
-
Mn2+-stimulated synthesis of cGMP is independent of IFNgamma/TNFalpha in astrocytes
Mn2+
-, Q0PY32
sGC requires a cation cofactor
Mn2+
-
the recombinant protein shows preference for Mn2+ than Mg2+ as cofactor for enzymatic activity
NaN3
-
activation
NaNO3
-
activation
Mn2+
-
dependent on
additional information
-
NO-independent, heme-dependent soluble guanylate cyclase
additional information
-
NO- and haem-independent soluble guanylate cyclase
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
17beta-estradiol
-
decreases enzyme activity in immature rats, while the amount of sGC alpha subuit increases, estrogen receptor-dependent effects, overview
1H-(1,2,4)-oxadiazole-(4,3-a)quinoxalin-1-one
-
-
1H-(1,2,4)oxadiazole(4,3-a)quinoxalin-1-one
-
-
1H-(1,2,4)oxadiazole(4,3-a)quinoxalin-1-one
-
-
1H-(1,2,4)oxadiazole(4,3-a)quinoxalin-1one
-
0.1 mM, complete inhibition
1H-(1,2,4)oxadiazole(4,3-a)quinoxalin-1one
-
-
1H-(1,2,4)oxadiazole(4,3-a)quinoxalin-1one
-
specific inhibitor of sGC
1H-[1,2,4] oxadiazole [4,3-a] quinoxalin-1-one
-
effectively blocks guanylyl cyclase activity in response to low O2 levels
1H-[1,2,4]-oxadiazole[4,3-a]quinoxalin-1-one
P19686, Q80WY4
i.e. ODQ, a highly selective sGC inhibitor; i.e. ODQ, a highly selective sGC inhibitor
1H-[1,2,4]oxadiazolo [4,3,-a]quinoxalin-1-one
Q02108, Q02153
i.e. ODQ, in vivo treatment with the sGC inhibitors ODQ does not decrease plasmatic and jejunal cGMP levels, nor does it influence the change in electrical field-induced relaxation; i.e. ODQ, in vivo treatment with the sGC inhibitors ODQ does not decrease plasmatic and jejunal cGMP levels, nor does it influence the change in electrical field-induced relaxation
1H-[1,2,4]oxadiazolo [4,3,-a]quinoxalin-1-one
-
i.e. ODQ
1H-[1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one
-
i.e. ODQ, a sGC inhibitor
1H-[1,2,4]oxadiazolo[4,3,-a]quinoxalin-1-one
-
i.e. ODQ
1H-[1,2,4]oxadiazolo[4,3-a]-quinoxalin-1-one
-
specific sGC inhibitor, significantly blocks the capsaicin-induced reduction of mechanical threshold to noxious stimulation of the masseter
1H-[1,2,4]oxadiazolo[4,3-a]-quinoxalin-1-one
-
guanylyl cyclase inhibitor, completely abrogates chemotactic and chemokinetic cellular movement to both diethylamine/NO and diethylenetriamine/NO exposure
1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin 1-one
-, Q8I7Z5
decreases infectivity of promastigotes
1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one
-
complete inhibition at 0.001 mM
1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one
-
i.e.ODQ, a sGC inhibitor
1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one
-
i.e. ODQ, inhibits the enzyme, and also inhibits Y-27632- and staurosporine-induced neurite outgrowth and triggered neurite retraction, but in an sGC-independent manner. The NO/sGC/cGMP signaling cascade is not, but the the ERK signaling pathway is critically involved in ODQ-induced neurite remodeling
1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one
-
-
1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one
-
-
1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one
-
a soluble guanylate cyclase inhibitor, maximal inhibition (70%) at 0.01 mM
1H-[1,2,4]oxadiazolo[4,3-a]quinoxaline-1-one
-
-
1H-[1,2,4]oxadiazolo[4,3a]quinoxalin-1-one
-
acute addition to pulmonary artery does not affect phenylephrine responsiveness, but restores the responsiveness in pulmonary artery treated with an NO-donor, overview
1H-[1,2,4]oxadiazolo[4,3a]quinoxalin-1-one
-
i.e. ODQ
1H-[1,2,4]oxadiazolo[4.3a]quinoxalin-1-one
-
inhibition of the enzyme is blocked by 8-bromo-cGMP or L-arginine
2'(3')-O-BODIPY-FL-guanosine 5'-[beta,gamma-imido]triphosphate
-
-
2',3'-O-(2,4,6-trinitrophenyl)-ADP
-
-
2',3'-O-(2,4,6-trinitrophenyl)-AMP
-
-
2',3'-O-(2,4,6-trinitrophenyl)-ATP
-
-
2',3'-O-(2,4,6-trinitrophenyl)-CTP
-
-
2',3'-O-(2,4,6-trinitrophenyl)-GDP
-
-
2',3'-O-(2,4,6-trinitrophenyl)-GTP
-
-
2',3'-O-(2,4,6-trinitrophenyl)-UTP
-
-
2'-dADP
-
mixture of competitive and noncompetitive inhibition, binding to the substrate site excludes the substrate GTP, whereas binding to the noncompetitive site has no effect on GTP binding, although the resulting complex is catalytically inactive. It binds to the high and low affinity sites with equivalent affinities. BAY 41-2272, which shares an analogous core structure to YC-1, fully inhibits 2'-dADP binding to the low affinity site, whereas the inhibition by YC-1 is incomplete
2'-deoxy-3'-O-(N-methylanthraniloyl)-ATP
-
-
2'-deoxy-3'-O-(N-methylanthraniloyl)-GDP
-
-
2'-deoxy-3'-O-(N-methylanthraniloyl)-GTP
-
-
2'-deoxy-3'-O-(N-methylanthraniloyl)-guanosine 5'-[beta,gamma-imido]triphosphate
-
-
2'-dUTP
-
-
3',5'-cyclic GMP
-
20 mM, 20-30% competitive inhibition
4H-8-bromo-1,2,4-oxadiazolo(3,4-d)benz(b)(1,4)oxazin-1-one
-
i.e. NS-2028, acts via the heme domain
4H-8-bromo-1,2,4-oxadiazolo(3,4-d)benz(b)(1,4)oxazin-1-one
Q8SPV3
i.e. NS-2028, acts via the heme domain
4H-8-bromo-1,2,4-oxadiazolo(3,4-d)benz(b)(1,4)oxazin-1-one
-
i.e. NS-2028, acts via the heme domain
4H-8-bromo-1,2,4-oxadiazolo(3,4-d)benz(b)(1,4)oxazin-1-one
-
NS-2028, a soluble guanylyl cyclase inhibitor, complete inhibition at 0.01 mM
6-(ethoxymethyl)-1-methyl-1,4,5,6-tetrahydropyrrolo[2,3-g]indazole
-
-
6-(ethoxymethyl)-1-phenyl-1,4,5,6-tetrahydropyrrolo[2,3-g]indazole
-
-
6-(ethoxymethyl)-5,6-dihydro-4H-[1,2]oxazolo[5,4-e]indole
-
-
6-(ethoxymethyl)-6H-[1,2]oxazolo[5,4-e]indole
-
-
6-anilino-5,8-quinolinedione
Q8SPV3
-
6-anilino-5,8-quinolinedione
-
-
6-anilino-quinoline-5-8-quinone
-, Q8I7Z5
decreases infectivity of promastigotes
6H-[1,2,4]oxadiazolo[4,3-d]pyrido[3,2-b][1,4]oxazin-9-one
-
-
8-(4-methoxyphenyl)-4H-[1,2,4]oxadiazolo[3,4-c][1,4]-benzoxazin-1-one
-
-
8-aza-3ATP
-
inhibition of basal and sodium nitroprusside activated sGC activity
8-bromo-4H-[1,2,4]oxadiazolo[3,4-c]benzoxazin-1-one
-
NS 2028
8-bromo-4H-[1,2,4]oxadiazolo[3,4-c][1,4]benzoxazin-1-one
-
i.e. NS-2028, a selective sGC inhibitor, 95% inhibition of enzyme activity at 0.01 mM, inhibits cell proliferation
8-Bromo-cGMP
-
-
8-oxoguanosine triphosphate
-
i.e. oxo8GTP, a potent inhibitor of nitric oxide-stimulated soluble guanylyl cyclase
8-oxoguanosine triphosphate
-
i.e. oxo8GTP, a potent competitive inhibitor of nitric oxide-stimulated soluble guanylyl cyclase
8-[3-(trifluoromethyl)phenyl]-4H-[1,2,4]oxadiazolo[3,4-c]-benzoxazin-1-one
-
-
9-chloro-12-oxo-6,12-dihydroquinazolino[2,3-c][1,4]-benzoxazine
-
-
adenosine 5'-beta,gamma-imido triphosphate
-
competitive inhibitior, reduces the Bmax for the binding of guanosine 5'-beta,gamma-methylene triphosphate, is tightly and selectively associated with the high affinity site
adenosine-5'-tetraphosphate
-
basal activity of wild-type enzyme is considerably less sensitive than NO-stimulated wild-type activity
ADPbetaS
-
basal activity of wild-type enzyme is considerably less sensitive than NO-stimulated wild-type activity
aldosterone
-
diminishes GC activity by activating NADPH oxidase in bovine aortic VSMC to increase ROS levels and induce oxidative posttranslational modification of Cys122, a beta1-subunit cysteinyl residue
ATP
-
allosteric inhibition of basal and sodium nitroprusside activated sGC activity
ATP
-
mechanism involving ATP pre-binding is physiologically relevant to activation of retinal guanylate cyclase, inhibition at high concentrations
ATP
-
in solubilized membranes ATP exerts an inhibitory role on basal and atrial natriuretic peptide 1-28-induced GC activity
ATP
-
80% inhibition at 0.1 mM, affects C-type NP 1-53 stimulation of the enzyme, in the presence of Mn2+ inhibition of mastoparan-induced activation of the enzyme
ATP
-
inhibition by ATP of GC activity stimulated by NO
ATP
Q81T60
inhibits the enzyme by 70% at 1 mM in presence of stoichiometric concentrations of NO
atrial natriuretic peptide
-
elicits GC-A desensitization, can induce decreased GC-A activity in MA-10 Leydig cells, based on mechanisms directly affecting the hormone responsiveness of the receptor. Protein kinase A blocks homologous atrial natriuretic peptide induced desensitization, in which cGMP is generated as second messenger
-
BaY 63-2521
-
the sGC inhibitor gives apositive effect in severe pulmonary hypertention patients
Benzamidine
-
-
BODIPY-FL-GTPgammaS
-
-
Ca2+
-
inhibition of both, the basal and NO-stimulated forms of sGC, competitive vs. Mg2+, noncompetitive vs. MgGTP
Ca2+
-
0.00005 mM, 50% inhibition
Ca2+
-
0.0002 mM, 50% inhibition
Ca2+
-
modulates the membrane guanylate cyclase transduction machinery by both inhibiting and stimulating it involving the guanylate cyclae, the guanylate cyclase activating protein type 1, S100B, and neurocalcin delta, mechanism, overview
Ca2+
-
the enzyme is inhibited by high intracellular Ca2+ concentration
Ca2+ ionophore A23187
-
Ca2+-dependent inhibition of sGC can be achieved with the Ca2+ ionophore A23187 (0.01 mM)
Calmidazolium
-
uncompetitive inhibition, time-dependent inhibition at 0.03 mM, pre-treatment of sGC with calmidazolium, followed by filtration, causes an 80% inhibition of sGC activation by nitric oxide and a 23% inhibition of activation by protoporphyrin IX
CO
-
forms stable complexes with Gyc-88E(1-597), inhibits 2.9fold
D-myo-Inositol 1,4,5-trisphosphate
-
-
diphosphate
-
-
diphosphate
-
-
diphosphate
-
-
diphosphate
-
competitive inhibition
DNIC-G
-
a dinitrosyl iron complex, solutions contain 18fold molar excess of free thiosulfate, inhibition of the NO-stimulated enzyme, inhibited by GSH
DNIC-Y
-
a dinitrosyl iron complex, no free thiosulfate in solutions of the binuclear complex DNIC-Y, inhibition of the NO-stimulated enzyme, inhibited by GSH
Fe2+
-
-
-
Fe3+
-
-
-
GDPbetaS
-
basal activity of wild-type enzyme is considerably less sensitive than NO-stimulated wild-type activity
Go6976
-
competitive inhibitor. Go6976 reduces GTP binding to the catalytic site of isoforms GC-A and GC-B. Inhibition of isoform GC-B is minimal in the absence of ATP and 1 mM ATP increases the inhibition 4fold
GTPgammaS
-
inhibits mastoparan-induced activation
guanosine 5'-(beta,gamma-imido)triphosphate
-
-
guanosine tetrakisphosphate
-
-
guanosine-5'-tetraphosphate
-
basal activity of wild-type enzyme is considerably less sensitive than NO-stimulated wild-type activity
guanylyl cyclase activating protein 1
-
GCAP1, a Ca2+/Mg2+-binding protein, metal binding in EF-hand 4 has no role in the primary attachment of GCAP1 to the cyclase, it only triggers the activator-to-inhibitor functional switch in GCAP1, differential binding of GCAP1 mutants, e.g. E75Q/E111Q/E155Q or D100N/D102G mutants, to RetGC1 in HEK-293 cells, overview
-
H2O2
-
diminishes GC activity by activating NADPH oxidase in bovine aortic VSMC to increase ROS levels and induce oxidative posttranslational modification of Cys122, a beta1-subunit cysteinyl residue
HS-142-1
-
natriuretic peptide released from the heart with acute heart failure target particulate GC, results in a decrease in UNaV and cGMP excretion together with a reduction in glomerular filtration rate and an increase in distal fractional tubular sodium reabsorption
-
Imidazole-HCl
-
-
ITP
-
basal activity of wild-type enzyme is considerably less sensitive than NO-stimulated wild-type activity
ITPgammaS
-
-
lauryldimethyl N-oxide
-
-
light
-
-
-
LY 83583
-
i.e. 6-anilino-5,8-quinoledione
LY-83583
-
inhibits the enzyme and completely blocks at 5 mM high frequency stimuli at 20 Hz/20 s-induced gLTP in superior cervical ganglia isolated from control rats
LY83583
-
a selective sGC inhibitor
lysophosphatidic acid
-
preincubations for 30 min before assessments of atrial natriuretic peptide-induced cGMP generation reveals inhibitory effects, exerted in a dose-dependent manner, elicits GC-A desensitization, protein kinase A does not block lysophosphatidic acid-induced heterologous desensitization. MEK inhibitor PD 98059 does not protect against the lysophosphatidic acid-mediated decrease in GC-A hormone sensitivity
methylene blue
-
non-specific sGC inhibitor, significantly blocks the capsaicin-induced reduction of mechanical threshold to noxious stimulation of the masseter
methylene blue
-
a nonspecific sGC inhibitor
methylene blue
Q8SPV3
-
methylene blue
-
-
methylene blue
P19686, Q80WY4
inhibits by oxidation of the enzyme's ferrous heme; inhibits by oxidation of the enzyme's ferrous heme
methylene blue
Q02108, Q02153
in vivo treatment with the sGC inhibitor methylene blue does not decrease plasmatic and jejunal cGMP levels, nor does it influence the change in electrical filed stimulation-induced relaxation; in vivo treatment with the sGC inhibitor methylene blue does not decrease plasmatic and jejunal cGMP levels, nor does it influence the change in electrical filed stimulation-induced relaxation
metrizamide
-
-
Mg2+ATPgammaS
-
inhibits cyclase activity through a mixed, non-competitive mechanism, only observable under NO stimulation and not under basal conditions
N-benzyl-N-(2-chloroethyl)-1-phenoxypropan-2-amine
-
-
N-methylanthraniloyl-adenosine 5'-[beta,gamma-imido]triphosphate
-
-
N-methylanthraniloyl-ADP
-
-
N-methylanthraniloyl-ATP
-
-
N-methylanthraniloyl-GDP
-
-
N-methylanthraniloyl-GTP
-
-
N-methylanthraniloyl-GTPgammaS
-
-
N-methylanthraniloyl-guanosine 5'-[beta,gamma-imido]triphosphate
-
-
N-methylanthraniloyl-ITPgammaS
-
-
N-methylanthraniloyl-xanthosine 5'-[beta,gamma-imido]triphosphate
-
-
N-methylanthraniloyl-XDP
-
-
NaCl
-
80% inhibition at 0.2 mM, affects C-type NP 1-53 stimulation of the enzyme
nitric oxide
-
-
NO
-
forms stable complexes with Gyc-88E(1-597), inhibits 2fold
NO
-
long acting NO donors pentaerythrityl tetranitrate and isosorbide mononitrate have a significant impact on the regulation of vascular sGC expression and activity, overview
NS-2028
-, Q0PY32
a potent sGC inhibitor
O2
-
forms stable complexes with Gyc-88E(1-597), inhibits 3.2fold
octylamine
-
-
ONE-GC 880MGTTVEPEYFDQVTIYFSDIVG901
-
causes ca. 90% inhibition
ONE-GC 900VGFTTISALSEPIEVVGFLNDL921
-
most effective inhibitor, causes ca. 95% inhibition
oxaloacetate
-
-
phosphodiesterase inhibitor dipyridamole
-
-
-
phosphoenolpyruvate
-
-
phosphoenolpyruvate
-
-
polylysine
-
-
potassium thiocyanate
-
-
protein phosphatase from spermatozoa
-
-
-
S-nitrosocysteine
-
inhibition of sGC activity by S-nitrosocysteine occurs only in parallel with the loss of cellular glutathione suggesting that loss of sGC activity may occur as a result of the severe depletion of the reduced thiol pool that occurs after exposure of cells to S-nitrosocysteine
sGC alpha1 splice variant
-
inhibits the enzyme, no co-precipitation of the N1-alpha1 sGC with the beta-subunit
-
Sodium azide
-
-
sodium lauryl sarcosinate
-
-
superoxide
Q8SPV3
-
tetanus toxin
Q9VEU5, Q9VEU6
expression of tetanus toxin in the neurons, that express isozyme Gyc-89Da, blocks their synaptic activity; expression of tetanus toxin in the neurons, that express isozyme Gyc-89Db, blocks their synaptic activity
-
thrombospondin-1
-
thrombospondin-1 is a universal inhibitor of sGC, blocking both hemedependent and heme-independent activation
-
Trifluoperazine
-
-
UTPgammaS
-
-
XDP
-
basal activity of wild-type enzyme is considerably less sensitive than NO-stimulated wild-type activity
XTP
-
basal activity of wild-type enzyme is considerably less sensitive than NO-stimulated wild-type activity
zinc (II) protophorphyrin IX
-
at higher concentrations, 2.5 M
Zn2+
-
0.08 mM, 50% inhibition of the intracellular domain of GCC catalytic activity
[1,2,4]-oxadiazolo[4,3-a]quinoxalin-1-one
-
inhibits the enzyme and the enzyme activating effect of sodium nitroprusside
[1,2,4]-oxadiazolo[4,3-a]quinoxalin-1-one
-
i.e. QDC, a selective sGC inhibitor, inhibits cell proliferation
[1,2,4]-oxadiazolo[4,3-a]quinoxalin-1-one
-
-
[1,2,4]-oxadiazolo[4,3-a]quinoxalin-1-one
Q8SPV3
-
[1,2,4]-oxadiazolo[4,3-a]quinoxalin-1-one
-
-
[1,2,4]-oxadiazolo[4,3-a]quinoxalin-1-one
B2MVM0
-
[1,2,4]-oxadiazolo[4,3-a]quinoxalin-1-one
-
-
[1,2,4]-oxadiazolo[4,3-a]quinoxalin-1-one
-
-
[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one
-
-
Mg2+GTPgammaS
-
inhibits cyclase activity through a mixed, non-competitive mechanism, only observable under NO stimulation and not under basal conditions
additional information
-
heme moiety of sGC has some inhibitory function on sGC activity
-
additional information
-
the N-terminal heme-bound regulatory domain of the beta1 subunit of soluble guanylate cyclase inhibits the activity of the alphacatbetacat complex in trans, suggesting a domain-scale mechanism of regulation by NO
-
additional information
-, Q8I7Z5
native and recombinant enzyme unaffected by chlorpromazine and trifluoprazine
-
additional information
-
phosphorylation at Ser64 of the alpha1 subunit inhibits the enzyme
-
additional information
-
competitive GC-C isozyme inhibition by exogenous 18-residue heat-stable enterotoxins, STa, produced by diarrheagenic bacteria, binding to GC-C by quantifying competitive displacement of [125I]STa from GC-C expressed in membranes, overview
-
additional information
-
competitive GC-C isozyme inhibition by exogenous 18-residue heat-stable enterotoxins, STa, produced by diarrheagenic bacteria, Ki values of 1.5-57.6 nM, binding to GC-C by quantifying competitive displacement of [125I]STa from GC-C expressed in membranes, overview
-
additional information
-
desensitization of the soluble guanylyl cyclase/cGMP pathway by lipopolysaccharide in rat isolated pulmonary artery but not aorta
-
additional information
-
effects of activators and inhibitors on enzyme regulation, overview
-
additional information
Q8SPV3
effects of activators and inhibitors on enzyme regulation, overview
-
additional information
-
effects of activators and inhibitors on enzyme regulation, overview
-
additional information
-
c-Src-dependent phosphorylation of sGC in induced by acetylcholine and inhibits NO-sensitive sGC activity and cGMP production
-
additional information
-, Q0PY32
sGC is influenced by the light conditions, overview
-
additional information
-
inhibition of the enzyme is involved in inhibition of tumor cell migration in lung
-
additional information
-
desensitization of the enzyme to NO and inactivation of soluble guanylate cyclase by stoichiometric S-nitrosation through dinitrosyl iron complexes, which is slowly reversible by GSH, overview
-
additional information
-
12-oxo-6,12-dihydroquinazolino[2,3-c][1,4]benzoxazine, 2-bromo-12-oxo-6,12-dihydroquinazolino[2,3-c][1,4]-benzoxazine, and 8-bromo-2,4-dihydro-1H-[1,2,4]triazolo[3,4-c][1,4]-benzoxazin-1-one do not exhibit an inhibitory effect on sGC
-
additional information
-
inhibition of soluble guanylyl cyclase requires binding to cell-surface receptor CD47. A thrombospondin-1 C-terminal fragment (E3CaG1) readily inhibits sGC in Jurkat T cells. This inhibition requires an increase in intracellular Ca+ concentration. Addition of angiotensin II also strongly inhibits soluble guanylyl cyclase activity by increasing intracellular Ca+ concentration
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
(2H-1-benzopyran-3(4H)-7'-bicyclo[4.2.0]-octa[1,3,5]-trien)4-one
-
activates the soluble guanylate cyclase exhibiting a vasorelaxing effect on the aortic ring, the compound is isolated from bulbs of South African Hyacinthaceae, i.e. Drimiopsis maculata, Eucomis schiffii, Urginea epigea, Drimia altissima, mass spectrometric identification. The compound counteracts vasocontractory agents, overview
-
(Z)-1-(N,N-diethylamino)diazen-1-ium-1,2-diolate
-
sGC ferrous-nitrosyl complex adopts two 5-coordinate conformations, a lower activity closed form, which releases NO slowly, and a higher activity open form, which releases NO rapidly
1-benzyl-3-(hydroxymethyl-2-furyl)indazole
-
trivial name YC-1, allosteric activator, synergistically increases the catalytic activity in the presence of NO
2,2-diethyl-1-nitroso-oxyhydrazine
-
0.1 mM, 87fold increase in activity, NO-relasing substance
2,2-diethyl-1-nitroso-oxyhydrazine
-
NO donor, 0.1 mM, 242fold activation
2,2-diethyl-1-nitroso-oxyhydrazine
-
70-80% activation at 0.01 mM in presence of 2 mM GSH
2-(N,N-diethylamino)-diazenolate-2-oxide
-
stimulates 4.3fold
-
2-(N,N-diethylamino)diazenolate-2-oxide
-
the wild type enzyme shows 59fold stimulation of activity at 0.001 mM 2-(N,N-diethylamino)diazenolate-2-oxide
-
2-[1-[(2-fluorophenyl)methyl]-1H-pyrazolo[3,4-b]pyridin-3-yl]-5(4-morpholinyl)-4,6-pyrimidinediamine
-
trivial name BAY 41-8543, 66fold activation at 0.1 mM, strong synergistic activation in the presence of NO
3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole
-
0.2 mM, 80fold activation of recombinant sGC
3-(5'-hydroxymethyl-2'-furylf)-1-benzylimdazole
-
activation
3-(5'-hydroxymethyl-2'furyl)-1-benzyl-indazole
-
0.1 mM, approx. 50fold stimulation, more than 2000fold stimulation in the presence of 0.1 mM sodium prusside
3-(5'-hydroxymethyl-3'-furyl)-1-benzylindazole
-
-
3-ethyl-3-(ethylaminoethyl)-1-hydroxy-2-oxo-1-triazene
P16068, P19687
stimulates via release of NO; stimulates via release of NO
-
3-ethyl-3-(ethylaminoethyl)-1-hydroxy-2-oxo-1-triazene
-
i.e. NOC-12
-
3-morpholinosydnonimine
-
i.e. SIN-1
4-((4-carboxybutyl){2-[(4-phenethyl-benzyl)oxy]-phenethyl}amino)methyl[benzoic]acid
-
NO- and heme-independent sGC activator, stimulates 15fold, stimulation increases up to 30fold in the presence of 1H-(1,2,4)-oxadiazole-(4,3-a)-quinoxalin-1-one
4-[((4-carboxybutyl){2-[(4-phenethylbenzol)oxy]phenethyl}amino)methyl] benzoic acid
-
trivial name BAY 58-2667, heme-independent activator, 30fold activation at 0.01 mM
5-cyclopropyl-2-[1-(2-fluoro-benzyl)-1H-pyrazolo[3,4-b]pyridin-3-yl]-pyrimidin-4-ylamine
-
NO-independent but heme-dependent sGC stimulator, stimulates the enzyme in a concentration-dependent manner
5-cyclopropyl-2-[1-(2-fluorobenzyl)-1H-pyrazolo[3,4-b]pyridin-3-yl]-pyrimidin-4-ylamine
-
-
5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol
-
the wild type enzyme shows 6fold stimulation of activity at 0.1 mM
-
A-350619
-
a heme-dependent stimulator of sGC, structurally not related to YC-1, but synergistic to YC-1 and sodium nitroprusside for the binding site
-
A-350619
Q8SPV3
a heme-dependent stimulator of sGC, structurally not related to YC-1, but synergistic to YC-1 and sodium nitroprusside for the binding site
-
A-350619
-
a heme-dependent stimulator of sGC, structurally not related to YC-1, but synergistic to YC-1 and sodium nitroprusside for the binding site
-
A-350619
-
a heme-dependent stimulator of sGC, structurally not related to YC-1, but synergistic to YC-1 and sodium nitroprusside for the binding site, 70fold activation as sole stimulator, 160fold in presence of YC-1 and 230fold in presence of sodium nitroprusside
-
A-778935
-
i.e. cis-3-[2-(2,2-dimethyl-propylsulfanyl)pyridin-3-yl]-N-(3-hydroxycyclohexyl-)acrylamide, derived from the YC-1 structure, activates the enzyme is a synergistic fashion with the NO donor sodium nitroprusside
-
A-778935
Q8SPV3
i.e. cis-3-[2-(2,2-dimethyl-propylsulfanyl)pyridin-3-yl]-N-(3-hydroxycyclohexyl-)acrylamide, derived from the YC-1 structure, activates the enzyme is a synergistic fashion with the NO donor sodium nitroprusside
-
A-778935
-
i.e. cis-3-[2-(2,2-dimethyl-propylsulfanyl)pyridin-3-yl]-N-(3-hydroxycyclohexyl-)acrylamide, derived from the YC-1 structure, activates the enzyme is a synergistic fashion with the NO donor sodium nitroprusside
-
Activator protein
-
from rat lung
-
adenosine 5'-[beta,gamma-imido]triphosphate
-
activation
adenylylimidophosphate
-
activation
arachidonic acid peroxide
-
activation
ataciguat
-
HMR 1766
-
ATP
-
mechanism involving ATP pre-binding is physiologically relevant to activation of retinal guanylate cyclase, inhibition at high concentrations
ATP
-
induces a concentration-dependent increase in basal and atrial natriuretic peptide 1-28-stimulated GC activity of glomerular and papillary membranes that is significantly higher in spontaneously hypertensive rats than in age-matched Wistar Kyoto rats
ATP
-
ATP-binding pocket and second, the transduction region, structure modelling, overview. The binding pocket resides in the smaller, N-terminal lobe of the ARM and the transduction region in the larger, predominantly helical, C-terminal lobe, overview. ATP-dependent mode of ANF-RGC signal transduction mechanism
ATP(gamma)S
-
activation
ATPgammaS
-
is more effective than ATP on glomerular and papillary membranes, has little effect on GC activity in solubilized membranes
Atrial natriuretic factor
-
binding the hormone to the GC-A extracellular domain activates its intracellular catalytic domain, overview
-
atrial natriuretic peptide
-
activation of the particulate form
-
atrial natriuretic peptide
-
activation of isoforms GC-A and GC-B
-
atrial natriuretic peptide
-
-
-
atrial natriuretic peptide
-
highly effective in stimulating cGMP production in MA-10 cells
-
atrial natriuretic peptide
-
increases cGMP production up to 300fold
-
atrial natriuretic peptide
-
-
-
atrial natriuretic peptide
-
-
-
atrial natriuretic peptide 1-28
-
increases GC enzymatic activity of glomerular and papillary membranes in a concentration-dependent manner
-
atrial NP 1-28
-
-
-
B-type natriuretic peptide
P18293
-
-
BAY 41-2272
-
sGC stimulant, relaxes newborn, but not adult bronchial muscle
BAY 41-2272
-
one molecule is required for maximal enzyme activation and is tightly associated with sGC
BAY 41-2272
-
a soluble guanylyl cyclase stimulator, it attenuates ischemia/reperfusion phorbol-12-myristate-13-acetate-induced lung injury by interfering with the activation NADPH oxidases
BAY 41-2272
-
an allosteric activator
BAY 41-2272
-
a direct sGC activator
BAY 41-2272
Q8SPV3
is effective against pulmonary hypertension in an ovine model
BAY 41-2272
-
is effective against pulmonary hypertension in a rat model
BAY 41-2272
-
-
BAY 41-2272
-
stimulator of the NO-independent, heme-dependent soluble guanylate cyclase, acts independently of and in synergism with NO producing anti-aggregatory, anti-proliferative, and vasodilatory effects, overview
BAY 41-2272
-
allosteric activator
BAY 41-2272
-
i.e. 5-cyclopropyl-2-[1-(2-fluorobenzyl)-1H-pyrazolo[3,4-b]pyridin-3-yl]pyrimidin-4-ylamine
BAY 41-2272
-
3-(4-amino-5-cyclopropylpyrimidin-2-yl)-1-(2-fluorobenzyl)-1H-pyrazolo[3,4-b]pyridine, synergistic activator of sGC
BAY 41-2272
-
sGC stimulator
BAY 41-2272
-
heme-dependent sGC activator
BAY 41-2272
-
riociguat, allosteric stimulator
BAY 41-8543
-
-
BAY 41-8543
-
lowers blood pressure in normotensive dogs
BAY 41-8543
Q8SPV3
-
BAY 41-8543
-
lowers blood pressure in normotensive rats
BAY 41-8543
-
-
BAY 41-8543
-
stimulator of the NO-independent, heme-dependent soluble guanylate cyclase, acts independently of and in synergism with NO producing anti-aggregatory, anti-proliferative, and vasodilatory effects, overview
BAY 41-8543
-
i.e. 2-[1-(2-fluorobenzyl)-1H-pyrazolo[3,4-b]pyridin-3-yl]-5-(4-morpholinyl)pyrimidine-4,6-diamine, a sGC stimulator
BAY 58-2667
-
-
BAY 58-2667
-
improves cardiac output accompanied with a drop in mean arterial pulmonary artery, right atrial, and pulmonary wedge pressure
BAY 58-2667
-
relaxes mesocolon specimen of type 2 diabetic patients, and induces potent arterial and venous vasodilation in patients with heart failure
BAY 58-2667
Q8SPV3
inhaled as microparticles, it is effective in eliciting pulmonary vasodilation in lambs
BAY 58-2667
-
long term treatment causes a slight decrease in systolic blood pressure
BAY 58-2667
-
activates the heme-free sGC 200fold, the activation is increased by inhibitor 1H-[1,2,4]oxidazolol[4,3a]quinoxalin-1-one
BAY 58-2667
-
activates the NO- and haem-independent soluble guanylate cyclase, the compound is capable of selectively activating the oxidized/haem-free enzyme via binding to the enzyme's haem pocket, causing pronounced vasodilatation
BAY 58-2667
-
cinaciguat, sGC activator
BAY 58-2667
-
cinaciguat, a soluble guanylate cyclase activator
BAY 60-2770
-
i.e. 4-([(4-carboxybutyl)[2-(5-fluoro-2-([4'-(trifluoromethyl)biphenyl-4-yl]methoxy)phenyl)ethyl]amino]methyl)benzoic acid, NO-independent activation, the compound attenuates pig serum-induced liver fibrosis in vivo but does not affect blodd pressure in spontaneously hypertensive rats, overview
-
BaY 63-2521
-
a heme-dependent stimulator of sGC
BaY 63-2521
-
i.e. methyl-4,6-diamino-2-(1-(2-fluorobenzyl)-1H-pyrazolo[3,4-b]pyridin-3-yl)pyrimindin-5-ylmethylcarbamate, stimulates sGC directly and sensitizes it to NO, causes enzyme upregulation in pulmonary arterial hypertension lungs
BaY 63-2521
-
stimulator of the NO-independent, heme-dependent soluble guanylate cyclase, acts independently of and in synergism with NO producing anti-aggregatory, anti-proliferative, and vasodilatory effects, overview
BaY 63-2521
-
i.e. methyl 4,6-diamino-2-[1-(2-fluorobenzyl)-1H-pyrazolo[3,4-b]pyridin-3-yl]pyrimidin-5-ylmethylcarbamate, analysis of safety, tolerability, pharmacokinetics, and pharmacodynamics and pharmacodynamic effects of the compound, which is beneficial in reduction of blood pressure, overview
BAY41-2272
-
i.e. 5-cyclopropyl-2-[1-(2-fluoro-benzyl)-1H-pyrazolo[3,4-b]pyridin-3-yl]-pyrimidin-4-ylamine
bicarbonate
-
activates cGMP-producing ability of guanylyl cyclase-D, a membrane guanylate cyclase exclusively expressed in the CO2-responsive olfactory sensory neurons, by directly acting on the intracellular cyclase domain of GC-D. The molecular mechanism for pH-independent GC-D activation is distinct from the release-from-repression model for other membrane guanylate cyclases, overview
brain natriuretic peptide
-
activation of isoforms GC-A and GC-B
-
brain natriuretic peptide
-
highly effective in stimulating cGMP production in MA-10 cells
-
C-type natriuretic peptide
-
-
-
C-type natriuretic peptide
-
activation of isoforms GC-A and GC-B
-
C-type natriuretic peptide
-
-
-
C-type NP 1-22
-
-
-
C-type NP 1-53
-
activation in a dose-dependent manner
-
carbon monoxide
-
-
CFM-1571
-
stimulator of the NO-independent, heme-dependent soluble guanylate cyclase, acts independently of and in synergism with NO producing anti-aggregatory, anti-proliferative, and vasodilatory effects, overview
-
cGMP
-
activation
cGMP
-
activation
cinaciguat
-
i.e. 4-([(4-carboxybutyl)[2-(2-[[4-(2-phenylethyl)benzyl]oxy]phenyl)ethyl]amino]methyl) benzoic acid or BAY 58-2667, analysis of safety, tolerability, pharmacokinetics, and pharmacodynamics of cinaciguat in treatment of patients with heart failure, the agent induces vasodilation preferentially in diseased vessels, overview
cinaciguat
-
BAY 58-2667
CO
-
activation
CO
-
activation
CO
-
activation
CO
-
2fold activation in absence of 3-(5'-hydroxymethyl-3'-furyl)-1-benzylimidazole, 115fold activation in presence of 0.15 mM 3-(5'-hydroxymethyl-3'-furyl)-1-benzylimidazole
cone-specific calcium sensor guanylate cyclase activating protein 4
-
encoded by gene zGCAP4 from zebrafish, cloning and expression in Escherichia coli, zGCAP4 is a strong activator of membrane-bound guanylate cyclases from bovine retina, mainly present as a monomer and showing half-maximal activation at 520-570 nM free Ca2+ concentration
-
cone-specific calcium sensor guanylate cyclase activating protein 4
-
encoded by gene zGCAP4 from zebrafish, cloning and expression in Escherichia coli, zGCAP4 is a strong activator of membrane-bound guanylate cyclases from zebrafish retina, mainly present as a monomer and showing half-maximal activation at 520-570 nM free Ca2+ concentration
-
CORM-2
-
i.e. CO-releasing molecule-2, a tricarbonyldichlororuthenium(II) dimer
-
diethylamine/NO
-
increases chemotaxis
diethylenetriamine nitric oxide
-
-
diethylenetriamine/NO
-
increases chemotaxis
G protein alpha subunit
Q8SPV3
-
-
G protein alpha subunit
-
-
-
GTPgammaS
-
activates by reducing the Km for GTP
GTPgammaS
-
activates by reducing the Km for GTP, half-maximal activation at 0.008 mM
GTPgammaS
-
stimulates activity of guanylyl cyclase GCA by lowering the KM-value of GTP and increasing the Vmax, half-maximal stimulation at 0.011 mM; stimulates activity of soluble guanylyl cyclase (sGC) by lowering the KM-value of GTP and increasing the Vmax, half-maximal stimulation at 0.008 mM
GTPgammaS
-
increases activity to 58%
guanosine 5'O-(thio)-triphosphate
-
activation
guanylate-activating protein
-
proteins GCAP-1 and GCAP-2, Ca2+-dependent negative feedback control
-
guanylin
-
activation of isoform GC-C
guanylyl cyclase activating protein
-
the native RetGC isozymes are accelerated 5-28fold at physiological concentrations of guanylyl cyclase activating proteins GCAP1 and GCAP2
-
guanylyl cyclase activating protein 1
-
GCAP1, a Ca2+/Mg2+-binding protein, after substitution of Ca2+ by Mg2+ in its EF-hands, stimulates photoreceptor guanylyl cyclase, RetGC1, in response to light. Metal binding in EF-hand 2 is crucial for GCAP1 attachment to RetGC1, while in EF-hand 3 it is less critical, although it enhances the efficiency of the GCAP1 docking on the target enzyme. Metal binding in EF-hand 4 has no role in the primary attachment of GCAP1 to the cyclase, it only triggers the activator-to-inhibitor functional switch in GCAP1, differential binding of GCAP1 mutants, e.g. E75Q/E111Q/E155Q or D100N/D102G mutants, to RetGC1 in HEK-293 cells, overview
-
H2O2
-
activates the enzyme, and reduces the cGMP formation activation by nitroprusside and BAY 41-2272 in smooth muscle cells, but enhances the response to HMR-1766, the activation by reactive oxigen species is heme-dependent
heat-stable enterotoxin
-
-
-
HMR 1766
-
activates the NO- and haem-independent soluble guanylate cyclase, compound is capable of selectively activating the oxidized/haem-free enzyme via binding to the enzyme's haem pocket, causing pronounced vasodilatation
HMR-1766
-
stimulates the enzyme, especially heme-free sGC, identification of the interaction region and binding structure of the enzyme by computational modelling
HMR-1766
-
chronic treatment causes reduced ex-vivo platelet adhesion and in vivo vasodilator-stimulated phosphoprotein phosphorylation
HMR-1766
-
causes long-lasting decrease in systolic blood pressure
Lubrol Px
-
activation
Mastoparan
-
most powerful activator, activation in a dose-dependent manner
menadione
-
-
N-(beta-D-glucopyranosyl-N2-acetyl-S-nitroso-D,L-penicillaminamide)
-
NO donor
N2,2'-O-dibutyryl-cGMP
-
activation
natriuretic peptide
-
the transcription factor Ets-1 regulates the natriuretic peptide receptor-A gene expression and the stimulation of the guanylate cyclase GC-A isozyme
-
natriuretic peptide
Q8SPV3
-
-
natriuretic peptide
-
-
-
natriuretic peptide
-
-
-
neurocalcin
-
in the presence of the semimicromolar range of free Ca2+, neurocalcin binds to the catalytic module and stimulates ONE-GC, M880-L921 region of ONE-GC is the Ca2+-dependent neurocalcin binding and the transduction site of ONE-GC
-
neurocalcin sigma
-
dose-dependent stimulation in presence of the 0.01 mM Ca2+, 8fold over the basal value at ca. 0.002 mM
-
neuronal calcium sensor GCAP1
-
confers Ca2+ sensitivity to guanylyl cyclase activity
-
neuronal calcium sensor GCAP2
-
confers Ca2+ sensitivity to guanylyl cyclase activity, absence of GCAP2 affected guanylyl cyclase activity in two ways: 1. the maximal rate of cGMP synthesis at low [Ca2] dropps 2fold and 2. the half-maximal rate of cGMP synthesis is attained at a higher than normal [Ca2+], phenotype of GCAP2 knockout mice, overview
-
nitric oxide
-
nitric oxide binds to its receptor soluble guanylyl cyclase and leads to 3',5'-cyclic-GMP production
nitric oxide
P20595
-
nitric oxide
-
-
nitric oxide
-
stimulates activity if sGC, 2.2 nM thrombospondin-1 binds to CD47 and potently inhibits nitric oxide stimulation of sGC
nitric oxide
-
-
nitroglycerin
-
induces vasorelaxation through release of NO, and causes reversible S-nitrosylation of sGC and desensitization in primary aortic smooth muscle cells, which is prevented by N-acetylcysteine
nitroprusside
-
-
nitrosobutane
-
6fold activation in absence of 3-(5'-hydroxymethyl-3'-furyl)-1-benzylimidazole, 47fold activation in presence of 0.15 mM 3-(5'-hydroxymethyl-3'-furyl)-1-benzylimidazole
nitrosohexane
-
2fold activation in absence of 3-(5'-hydroxymethyl-3'-furyl)-1-benzylimidazole, 33fold activation in presence of 0.15 mM 3-(5'-hydroxymethyl-3'-furyl)-1-benzylimidazole
nitrosooctane
-
2fold activation in absence of 3-(5'-hydroxymethyl-3'-furyl)-1-benzylimidazole, 11fold activation in presence of 0.15 mM 3-(5'-hydroxymethyl-3'-furyl)-1-benzylimidazole
nitrosopentane
-
2fold activation in absence of 3-(5'-hydroxymethyl-3'-furyl)-1-benzylimidazole, 39fold activation in presence of 0.15 mM 3-(5'-hydroxymethyl-3'-furyl)-1-benzylimidazole
nitrosopropane
-
2fold activation in absence of 3-(5'-hydroxymethyl-3'-furyl)-1-benzylimidazole, 45fold activation in presence of 0.15 mM 3-(5'-hydroxymethyl-3'-furyl)-1-benzylimidazole
NO
P33402
activation
NO
-
activation
NO
-
sGC activity increases 8fold upon binding of NO
NO
-
binds at beta1 subunit heme, several hundred fold increase in activity
NO
-
127fold activation in absence of 3-(5'-hydroxymethyl-3'-furyl)-1-benzylimidazole, 211fold activation in presence of 0.15 mM 3-(5'-hydroxymethyl-3'-furyl)-1-benzylimidazole
NO
-
binds to the heme cofactor in the beta1 subunit, forming a five-coordinate NO complex that activates the enzyme several hundred-fold
NO
-
NO binds to the heme of soluble guanylate cyclase heme, activating the enzyme. In the presence of physiological concentrations of ATP and GTP, NO dissociation from the heme of soluble guanylate cyclase is about 160 times slower than the rate of enzyme deactivation in vitro. Deactivated enzyme still has NO bound to the heme, and full activation requires additional NO. An activation model is proposed where, in the presence of both ATP and GTP, tonic NO forms a stable heme complex with low activity, acute production of NO transiently and fully activates this NO-bound enzyme
NO
-
perfect heme ligand, serves as an effector of enzyme activation via conformational transitions
NO
-
H-NOX has a very high affinity for NO, with a Kd in the femtomolar range, NO complex wiht H-NOX is very stable, five-coordinate NO complex
NO
-
perfect heme ligand, serves as an effector of enzyme activation via conformational transitions
NO
-
beta2 homodimer forms a five-coordinate Fe(II)-NO-complex
NO
-
is bound in the distal heme pocket of the L2 H-NOX fold, NO dissociates from the distal side of the heme in both 5- and 6-coordinate complexes
NO
-
conventional isoforms Gyca-99B and Gycb-100B are potently activated by NO. Atypical sGCs Gyc-88E Gyc-89Da Gyc-89Db are only slightly sensitive to NO. At atmospheric O2 concentrations, NO slightly stimulates the Gyc-88E+Gyc-89Da and Gyc-88E+Gyc-89Db subunit combinations, whereas at lower O2 concentrations NO inhibits their activity
NO
-
the enzyme activation by NO leads to increased pulmonary artery relaxation, the activation is inhibited by heme depletion through heme oxygenase-1 induction
NO
-
the sensitivity to NO is increased in case of obesity and high-fat diet
NO
-
binds and activates the enzyme
NO
-
nitric oxide is a modulator of neurotransmission in cardiac ganglia and in neural control of the adult human heart
NO
-
binding of NO to sGC leads to the formation of a five-coordinate ferrous-nitrosyl complex and a several hundred-fold increase in cGMP synthesis, the NO activation of sGC is influenced by GTP and the allosteric activators YC-1 and BAY 41-2272, analysis of the Fe-NO conformation, overview
NO
-
interacts with the heme cofactor via the heme NO oxygen domain, structural basis, thermodynamics, and kinetics, activation mechanism involving the alphaFbeta1 loop in the iron proximal histidine bond breaking process, overview
NO
-
NO indirectly inhibits 5-lipoxygenase metabolism synthesis via activation of soluble guanylyl cyclase in rat alveolar macrophages
NO
Q8SPV3
-
NO
-
NO-activated soluble guanylyl cyclase
NO
-
induces vasorelaxation
NO
-
NO, acting via isozyme sGCalpha1beta1, is the principal neurotransmitter in electrical field stimulation-evoked responses
NO
-
activates the soluble guanylate cyclase, the activation is abolished by 1H-[1,2,4]oxadiazolo[4.3a]quinoxalin-1-one
NO
-
about 5000fold activation over basal level, mechanism for NO receptor activation and its modulation by GTP, ATP, and allosteric agents, model formation comprising a module in which NO, the nucleotides, and allosteric agents bind and the protein undergoes a conformational change, dovetailing with a catalytic module where GTP is converted to cGMP and diphosphate, enzyme-linked receptor mechanism, overview
NO
Q81T60
170fold activation, binding of NO leads to a transient six-coordinate intermediate, followed by release of the proximal histidine to yield a five-coordinate nitrosyl complex. Hallmark of sGC activation by NO is the release of beta1 His105 from the heme, leading to allosteric stimulation of cyclase activity
NO
-
activates the soluble guanylyl cyclase
NO
-
activates sGC
NO
-, Q0PY32
NO stimulates elevation of endogenous cGMP in Pharbitis nil, which allows for stimulation of flower bud formation in non-inductive conditions
NO
-
binding of NO to a regulatory heme group at the beta-subunit of the protein results in 100fold stimulation of cGMP formation, NO is involved in the vascular NO/cGMP signalling, dysfunction of the signaling is thought to contribute to a wide variety of cardiovascular disorders
NO
-
activates the sGCalpha2beta1 enzyme and induces muscle relaxation after electric field stimulation
NO
-
nitric oxide-sensitive guanylate cyclase
NO
-
activation of sGC by NO, released from 2,2-diethyl-1-nitroso-oxyhydrazine, is a link to the nitroglycerin biotransformation by mitochondrial aldehyde dehydrogenase, ALDH2
NO
-
340fold activation
NO
Q02108, Q02153
;
O2
-
H-NOX forms/does not form a stable O2 complex at 70C, diverging studies
O2
-
atypical sGCs potently activated under hypoxic conditions
peroxynitrite
-
activation in presence of GSH
phenylhydrazine
-
acivation
prostaglandin endoepoxide analogs
-
activation
-
protoporphyrin IX
-
0.001 mM activates untreated sGC with around half the effectiveness of nitric oxide
protoporphyrin-IX
-
the wild type enzyme shows 1.9fold stimulation of activity at 0.1 mM and 12fold stimulation at 0.01 mM
riociguat
-
i.e. BAY 63-2521, a soluble guanylate cyclase stimulator, acts directly, stimulates the enzyme, and increases the sensitivity to low NO levels. It significantly improves pulmonary haemodynamic parameters and cardiac index in patients with pulmonary hypertension
S-nitroso-N-acetylpenicillamine
-
the wild type enzyme shows 14.5fold stimulation of activity at 0.1 mM
S-nitrosocysteine
-
induces vasorelaxation through release of NO, and causes reversible S-nitrosylation of sGC and desensitization in primary aortic smooth muscle cells, which is prevented by N-acetylcysteine
S-nitrosocysteine
-
activates soluble guanylyl cyclase at low concentration (0.02 mM), oxy-hemoglobin inhibits the ability of S-nitrosocysteine to activate sGC
S-nitrosoglutathione
-
stimulates the enzyme activity via NO production, stimulation is inhibited by acetylcholine
sodium nitroprusside
-
-
sodium nitroprusside
-
activation of the soluble form
sodium nitroprusside
-
activation of the soluble form
sodium nitroprusside
-
NO donor
sodium nitroprusside
-
NO donor
sodium nitroprusside
-
0.1 mM, 580fold stimulation, NO donor
sodium nitroprusside
-
NO donor, significant higher stimulation of sGC activity in newborns compared with adults
sodium nitroprusside
-
an NO donor
sodium nitroprusside
-
-
sodium nitroprusside
-
synergistic to YC-1 and A-350619 for the binding site
sodium nitroprusside
Q8SPV3
synergistic to YC-1 and A-350619 for the binding site
sodium nitroprusside
-
synergistic to YC-1 and A-350619 for the binding site
sodium nitroprusside
-
synergistic to YC-1 and A-350619 for the binding site, 230fold activation in presence of A-350619
sodium nitroprusside
-
-
sodium prusside
P19686, Q80WY4
activates via release of NO; activates via release of NO
-
sodium prusside
-, Q0PY32
via NO production
-
Triton X-100
-
activation
Triton X-100
-
activation
unsaturated fatty acids
-
activation
-
uroguanylin
-
stimulates ONE-GC through its external domain in a dose-dependent fashion
von Willebrand factor/ristocetin
-
increases the enzyme activity in an NO-independent manner correlating with Src kinase-dependent phosphorylation of sGC beta1-subunit-Tyr192
-
von Willebrand factor/ristocetin
-
increase the enzyme activity in an NO-independent manner correlating with Src kinase-dependent phosphorylation of sGC beta1-subunit-Tyr192
-
YC-1
-
sGC stimulant, relaxes newborn, but not adult bronchial muscle
YC-1
-
an allosteric activator
YC-1
-
a non-NO-dependent activator of sGC
YC-1
-
synergistic with NO, blocked by 1H-[1,2,4]oxidazolol[4,3a]quinoxalin-1-one
YC-1
Q8SPV3
synergistic with NO, blocked by 1H-[1,2,4]oxidazolol[4,3a]quinoxalin-1-one
YC-1
-
synergistic with NO, blocked by 1H-[1,2,4]oxidazolol[4,3a]quinoxalin-1-one
YC-1
-
synergistic with NO, blocked by 1H-[1,2,4]oxidazolol[4,3a]quinoxalin-1-one, from rats, synergistic to A-350619 and sodium nitroprusside for the binding site, 160fold activation in presence of A-350619
YC-1
-
stimulator of the NO-independent, heme-dependent soluble guanylate cyclase, acts independently of and in synergism with NO producing anti-aggregatory, anti-proliferative, and vasodilatory effects, overview
YC-1
-
allosteric activator
YC-1
Q81T60
affects binding of NO and CO to the enzyme, it reduces the NO and CO off-rates for the 100 kDa N-terminal heterodimeric fragment and increases the CO affinity by 50fold, overview
YC-1
-
an NO-independent activator of sGC
YC-1
-, Q0PY32
-
YC-1
-
i.e. 1-benzyl-3-(5'-hydroxymethyl-2'-furyl-)-indazol, a sGC sensitizer, able to potentiate CO- and CORM-2-induced relaxations in wild-type mice
YC-1
-
heme-dependent sGC activator
YC-1
-
activator of soluble guanylyl cyclase
YC-1
-
allosteric stimulator
[1,2,4]-oxadiazolo[4,3-a]quinoxalin-1-one
-
potentiates the activating effect of HMR-1766 on the wild-type enzyme
meso-porphyrin IX
-
heme-independent activator
additional information
-
the nitric oxide system is an activator of renal soluble GC
-
additional information
-
guanylin is totally ineffective in stimulating ONE-GC
-
additional information
-
C-type natriuretic peptide does not stimulate cGMP production
-
additional information
-, Q8I7Z5
insensitive to NO stimulation, bovine calmodulin fails to stimulate the enzyme
-
additional information
-
no affinity for O2, the mechanism of oxygen exclusion by sGC not only involves the lack of hydrogen bonding in the distal heme pocket, but also depends on structural elements from other domains of sGC
-
additional information
-
none of the atypical sGCs are activated by the NO-independent, but heme-dependent, sGC stimulator BAY 41-2272
-
additional information
-
insensitive to NO
-
additional information
-
GCC is activated by diarrheagenic bacterial heat-stable enterotoxins, which suppress proliferation of Caco-2 human colon carcinoma cells. Exisulind inhibits the ability of diarrheagenic bacterial heat-stable enterotoxin to induce maximal intracellular cGMP accumulation
-
additional information
-
NO treatment elevates the enzyme activity shortly after application, but does not significantly affect the steady-state levels of cGMP
-
additional information
-
synthesis of STa, exogenous heat-stable enterotoxin, fragments peptide 3 and peptide 6, which behave as agonists in stimulating cGMP production, overview
-
additional information
Q5YLC2
guanylate cyclase activation mechanism, overview
-
additional information
-
effects of activators and inhibitors on enzyme regulation, overview
-
additional information
Q8SPV3
effects of activators and inhibitors on enzyme regulation, overview
-
additional information
-
effects of activators and inhibitors on enzyme regulation, overview
-
additional information
-
tirofiban, which mimics the structure of arginine-glycine-aspartic acid peptides, up-regulates soluble guanylate cyclase beta1 subunit, sGC-beta1, expression in contractile vascular smooth muscle cells and in aorta from rats, and tirofiban reverses the down-regulation of soluble guanylate cyclase content promoted by chronic treatment with NO donors and NO donor tachyphylaxis
-
additional information
-
no nitrosylating and activating effect by S-nitrosoglutathione
-
additional information
-
electrical field stimulation partially activates the enzyme and induces smooth muscle relaxation, overview
-
additional information
-
identification of stimulating acryl-amide compounds, that act independently of YC-1 stimulators and promote smooth muscle relaxation
-
additional information
Q81T60
for maximal activity, msGC required both NO and YC-1
-
additional information
P18293
treatment of mice with angiotensin II results in enhanced pulmonary mRNA expression of spliced GC-A, which is concomitant to diminished GC-A/cGMP responses to atrial natriuretic peptide, overview
-
additional information
-
direct stimulation of cGMP synthesis and activation of mitogen-activated protein kinase signaling pathways underlies the capacity of Mn2+ to augment NF-kappaB-dependent gene expression in astrocytes
-
additional information
-
NO-independent sGC activation is synergistic with NO-sGC stimulation
-
additional information
P19686, Q80WY4
soluble GC alpha-1 and beta-1 subunit mRNA levels are increased in the lungs, but not in the aorta in a sepsis model, overview; soluble GC alpha-1 and beta-1 subunit mRNA levels are increased in the lungs, but not in the aorta in a sepsis model, overview
-
additional information
-, Q0PY32
sGC is influenced by the light conditions, overview
-
additional information
B2MVM2, -
soluble guanylyl cyclase beta-3 subunit responds poorly to nitric oxide
-
additional information
-
neither staurosporine nor Go6976 activate isoforms GC-A or GC-B
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0357
-
2'-O-(N-methylanthraniloyl) guanosine 5'-triphosphate
-
-
0.02
0.1
GTP
-
-
0.02
0.1
GTP
-
-
0.021
-
GTP
-
pH 8.0, 30C, wild-type enzyme, in presence of NO-donor S-nitroso-N-acetylpenicillamine
0.025
-
GTP
-
30C, pH 8.0, in the presence of N-(beta-D-glucopyranosyl-N2-acetyl-S-nitroso-D,L-penicillaminamide) and YC-1
0.034
-
GTP
-
-
0.039
-
GTP
-
30C, pH 8.0, in the presence of YC-1
0.043
-
GTP
P16068, P19687
purified recombinant sGC in presence of 3-ethyl-3-(ethylaminoethyl)-1-hydroxy-2-oxo-1-triazene; purified recombinant sGC in presence of 3-ethyl-3-(ethylaminoethyl)-1-hydroxy-2-oxo-1-triazene
0.0498
-
GTP
-
pH 7.5, 37C, NO-stimulated recombinant enzyme
0.057
-
GTP
-
in the presence of NO
0.061
-
GTP
-
30C, pH 8.0, in the presence of N-(beta-D-glucopyranosyl-N2-acetyl-S-nitroso-D,L-peniciiaminamide)
0.065
-
GTP
-
recombinant sGC
0.069
-
GTP
-
in the absence of Ca2+ and in the presence of 0.05 mM nitric oxide, in 50 mM HEPES, pH 7.5, at 37C
0.07
-
GTP
-
octylglucoside solubilized enzyme, Mn2+-supported enzyme
0.074
-
GTP
-
at the basal state of sGC
0.085
-
GTP
-
Mn2+-GTP
0.0901
-
GTP
-
pH 7.5, 37C, not stimulated recombinant enzyme
0.095
-
GTP
-
-
0.12
-
GTP
-
30C, pH 8.0, basal activity
0.122
-
GTP
-
pH 8.0, 30C, wild-type enzyme
0.134
-
GTP
-
37C, pH 7.4, recombinant sGC
0.14
-
GTP
-
native enzyme, Mn2+-supported enzyme
0.16
-
GTP
-
37C, pH 7.5, in the absence of ATP
0.18
-
GTP
P16068, P19687
purified recombinant sGC; purified recombinant sGC
0.19
-
GTP
-
in the absence of NO
0.23
-
GTP
-
-
0.234
-
GTP
-
in the absence of Ca2+ and nitric oxide, in 50 mM HEPES, pH 7.5, at 37C
0.25
-
GTP
-
22C, pH 8.0
0.25
-
GTP
-
guanylyl cyclase GCA
0.36
-
GTP
-
37C, pH 7.5, in the presence of 1 mM ATP
0.386
-
GTP
-, Q8I7Z5
purified enzyme
0.4
-
GTP
-
native enzyme, Mg2+-supported enzyme
0.401
-
GTP
-
octylglucoside solubilized enzyme, Mg2+-supported enzyme
0.4077
-
GTP
-
Wistar Kyoto rats, in presence of atrial natriuretic peptide 1-28 and ATP in glomerular membranes
0.41
-
GTP
-
22C, pH 8.0
0.414
-
GTP
-
soluble guanylyl cyclase sGC
0.455
-
GTP
-, Q8I7Z5
recombinant enzyme
0.5
-
GTP
-, Q0PY32
pH 7.6, 30C
0.5597
-
GTP
-
Wistar Kyoto rats, in presence of atrial natriuretic peptide 1-28 in glomerular membranes
0.63
-
GTP
-
isozyme RetGC2, stimulated by guanylyl cyclase activating protein 1,in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30C
0.64
-
GTP
-
isozyme RetGC1, stimulated by guanylyl cyclase activating protein 1, in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30C
0.66
-
GTP
-
with 0.273 mM oxygen
0.667
-
GTP
-
Wistar Kyoto rats, in presence of ATP in glomerular membranes
0.7
-
GTP
-
isozyme RetGC1, stimulated by guanylyl cyclase activating protein 2, in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30C
0.79
-
GTP
-
isozyme RetGC2, stimulated by guanylyl cyclase activating protein 2,in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30C
0.8
-
GTP
-
-
0.82
-
GTP
-
under anaerobic conditions
0.8234
-
GTP
-
Wistar Kyoto rat, basal GC activity of glomerular membranes
0.857
-
GTP
-
in the presence of 2 mM Ca2+ and in the absence of nitric oxide, in 50 mM HEPES, pH 7.5, at 37C
0.887
-
GTP
-
in the presence of 2 mM Ca2+ and 0.05 mM nitric oxide, in 50 mM HEPES, pH 7.5, at 37C
1.38
-
GTP
Q7CH76
wild type enzyme, pH 8.5, 37C
1.55
-
GTP
-
nonstimulated isozyme RetGC1, in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30C
3.18
-
GTP
-
nonstimulated isozyme RetGC2, in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30C
0.37
-
guanyl-(beta,gamma-methylene)-diphosphonate
-
-
0.07
-
guanyl-imidodiphosphate
-
-
0.085
-
MgGTP2-
-
-
0.09
-
MgGTP2-
P33402
-
0.1
-
MgGTP2-
P33402
alpha2,beta1 isoform
0.14
-
MgGTP2-
-
-
1.07
-
MgGTP2-
-
-
0.01
-
MnGTP2-
-
-
0.037
-
MnGTP2-
-
soluble form
0.17
-
MnGTP2-
-
-
0.25
-
MnGTP2-
-
-
0.274
-
MnGTP2-
-
-
0.752
-
MnGTP2-
-
particulate form
additional information
-
additional information
Q5YLC2
the soluble enzyme has two active sites in the dimer exhibiting positive cooperativity with a Hill coefficient of about 1.5
-
additional information
-
additional information
-
activation kinetics and thermodynamics, binding of GTP and NO, overview
-
additional information
-
additional information
Q81T60
kinetics of ligand binding
-
additional information
-
additional information
P16068, P19687
kinetics, purified recombinant sGC, overview; kinetics, purified recombinant sGC, overview
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.15
-
GTP
-
pH 7.5, 37C, not stimulated recombinant enzyme
2.6
-
GTP
-
nonstimulated isozyme RetGC1, in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30C
20
-
GTP
-
nonstimulated isozyme RetGC2, in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30C
28.7
-
GTP
-
pH 7.5, 37C, NO-stimulated recombinant enzyme
33
-
GTP
-
isozyme RetGC1, stimulated by guanylyl cyclase activating protein 2, in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30C
74
-
GTP
-
isozyme RetGC1, stimulated by guanylyl cyclase activating protein 1, in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30C
94
-
GTP
-
isozyme RetGC2, stimulated by guanylyl cyclase activating protein 2,in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30C
116
-
GTP
-
isozyme RetGC2, stimulated by guanylyl cyclase activating protein 1,in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30C
290
-
GTP
-
pH 7.4, 30C
3.9
-
MgGTP2-
-
-
0.2
1.3
MnGTP2-
-
-
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.00105
-
GTP
Q7CH76
wild type enzyme, pH 8.5, 37C
11186
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.3
-
2'(3')-O-BODIPY-FL-guanosine 5'-[beta,gamma-imido]triphosphate
-
pH 7.4, 30C
0.00071
-
2',3'-O-(2,4,6-trinitrophenyl)-ATP
-
in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30C
0.0051
-
2',3'-O-(2,4,6-trinitrophenyl)-CTP
-
in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30C
0.0072
-
2',3'-O-(2,4,6-trinitrophenyl)-GTP
-
in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30C
0.00311
-
2',3'-O-(2,4,6-trinitrophenyl)-UTP
-
in 10 mM MnCl2, 100 mM KCl, 25 mM HEPES/NaOH, pH 7.4, at 30C
3.6
-
2'-deoxy-3'-O-(N-methylanthraniloyl)-ATP
-
pH 7.4, 30C
16
-
2'-deoxy-3'-O-(N-methylanthraniloyl)-GDP
-
pH 7.4, 30C
0.22
-
2'-deoxy-3'-O-(N-methylanthraniloyl)-GTP
-
pH 7.4, 30C
0.53
-
2'-deoxy-3'-O-(N-methylanthraniloyl)-guanosine 5'-[beta,gamma-imido]triphosphate
-
pH 7.4, 30C
24
-
2'-dUTP
-
pH 7.4, 30C
0.0051
-
AMP-PNP
-
at 10C
0.015
-
AMP-PNP
-
at 27C
2.3
-
ATP
-
37C, pH 7.5
23
-
BODIPY-FL-GTPgammaS
-
pH 7.4, 30C
0.00005
-
Ca2+
-
; soluble guanylyl cyclase sGC
0.0002
-
Ca2+
-
soluble guanylyl cyclase sGC
0.029
-
Ca2+
-
37C, pH 7.4, competitive vs. Mg2+
0.096
-
Ca2+
-
37C, pH 7.4, noncompetitive vs. MgGTP
0.3
-
diphosphate
-
-
0.001
-
Go6976
-
in the presence of 1 mM ATP, isozyme GC-A, in 25 mM HEPES (pH 7.4), 50 mM NaCl, 0.1% (w/v) bovine serum albumin, 0.5 mM isobutylmethyl xanthine, 1 mM EDTA, 0.5 mM microcystin and 5 mM MgCl2, at 37C
0.0013
-
Go6976
-
in the presence of 1 mM ATP, isozyme GC-B, in 25 mM HEPES (pH 7.4), 50 mM NaCl, 0.1% (w/v) bovine serum albumin, 0.5 mM isobutylmethyl xanthine, 1 mM EDTA, 0.5 mM microcystin and 5 mM MgCl2, at 37C
3.2
-
ITPgammaS
-
pH 7.4, 30C
0.23
-
N-methylanthraniloyl-adenosine 5'-[beta,gamma-imido]triphosphate
-
pH 7.4, 30C
0.4
-
N-methylanthraniloyl-ADP
-
pH 7.4, 30C
0.43
-
N-methylanthraniloyl-ATP
-
pH 7.4, 30C
4.2
-
N-methylanthraniloyl-GDP
-
pH 7.4, 30C
0.71
-
N-methylanthraniloyl-GTP
-
pH 7.4, 30C
1.1
-
N-methylanthraniloyl-GTPgammaS
-
pH 7.4, 30C
1.5
-
N-methylanthraniloyl-guanosine 5'-[beta,gamma-imido]triphosphate
-
pH 7.4, 30C
4.9
-
N-methylanthraniloyl-ITPgammaS
-
pH 7.4, 30C
1.7
-
N-methylanthraniloyl-xanthosine 5'-[beta,gamma-imido]triphosphate
-
pH 7.4, 30C
2
5
N-methylanthraniloyl-XDP
-
pH 7.4, 30C
16
-
UTP
-
pH 7.4, 30C
4.1
-
UTPgammaS
-
pH 7.4, 30C
0.251
-
Mg2+GTPgammaS
-
pH 8.0, 30C, wild-type enzyme
additional information
-
additional information
-
inhibition kinetics
-
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.288
-
8-oxoguanosine triphosphate
-
pH 7.4, 37C, in presence of 0.05 mM GTP
1.8
-
8-oxoguanosine triphosphate
-
pH 7.4, 37C, in presence of 0.5 mM GTP
0.06
-
900VGFTTISALSEPIEVVGFLNDL921
-
-
-
0.07
-
ATP
-
-
0.01
-
Calmidazolium
-
purified sGC
0.001
-
DNIC-G
-
pH 7.4, 37C, inhibition of NO-stimulated enzyme
0.01
-
DNIC-Y
-
pH 7.4, 37C, inhibition of NO-stimulated enzyme
0.002
-
Go6976
-
in the presence of 1 mM ATP, isozyme GC-B, in 25 mM HEPES (pH 7.4), 50 mM NaCl, 0.1% (w/v) bovine serum albumin, 0.5 mM isobutylmethyl xanthine, 1 mM EDTA, 0.5 mM microcystin and 5 mM MgCl2, at 37C
0.07
-
NaCl
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.00000045
-
-, Q0PY32
activity in cotyledons
0.00000048
-
-, Q0PY32
activity in seedlings
0.0056
-
-
basal sGC activity
0.0096
-
-
recombinant intracellular domain of GCC
0.022
-
P16068, P19687
purified recombinant sGC; purified recombinant sGC
0.034
-
-
basal activity in the absence of NO
0.041
-
-
-
0.047
-
-
mutant enzyme D102N, at pH 8.5 and 33C, in the absence of any stimulator
0.073
-
-
mutant enzyme D102E, at pH 8.5 and 33C, in the absence of any stimulator
0.087
-
-
mutant enzyme D102N, at pH 8.5 and 33C, in the presence of 0.1 mM protoporphyrin-IX
0.108
-
-
mutant enzyme F120A, at pH 8.5 and 33C, in the presence of 0.1 mM protoporphyrin-IX
0.11
-
-
-
0.12
-
-
basal activity
0.128
-
-
mutant enzyme D102E, at pH 8.5 and 33C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol
0.152
-
-
mutant enzyme D102E, at pH 8.5 and 33C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol
0.16
-
-
mutant enzyme D102A, at pH 8.5 and 33C, in the presence of 0.1 mM protoporphyrin-IX
0.161
-
-
mutant enzyme D102A, at pH 8.5 and 33C, in the absence of any stimulator
0.168
-
-
mutant enzyme D102E, at pH 8.5 and 33C, in the presence of 0.1 mM protoporphyrin-IX
0.169
-
-
mutant enzyme F120A, at pH 8.5 and 33C, in the absence of any stimulator
0.172
-
-
mutant enzyme F120A, at pH 8.5 and 33C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol
0.202
-
-
mutant enzyme D102N, at pH 8.5 and 33C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol
0.205
-
-
-
0.229
-
-
mutant enzyme D102N, at pH 8.5 and 33C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol; wild type enzyme, at pH 8.5 and 33C, in the absence of any stimulator
0.231
-
-
mutant enzyme F120A, at pH 8.5 and 33C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol and 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol
0.276
-
-
with Mn2+
0.278
-
-
mutant enzyme F120A, at pH 8.5 and 33C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol
0.287
-
-
-
0.29
-
-
pulmonary artery homogenate in absence of NO
0.292
-
-
mutant enzyme D102E, at pH 8.5 and 33C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol and 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol
0.354
-
-
mutant enzyme D102A, at pH 8.5 and 33C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol
0.431
-
-
wild type enzyme, at pH 8.5 and 33C, in the presence of 0.1 mM protoporphyrin-IX
0.53
-
-
pulmonary artery homogenate after 24 h of treatment with NO
0.662
-
-
mutant enzyme D102A, at pH 8.5 and 33C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol and 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol
0.693
-
-
-
0.94
-
-
-
1.063
-
-
mutant enzyme D102N, at pH 8.5 and 33C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol and 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol
1.232
-
-
mutant enzyme D102A, at pH 8.5 and 33C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol
1.33
-
P16068, P19687
purified recombinant sGC in presence of 3-ethyl-3-(ethylaminoethyl)-1-hydroxy-2-oxo-1-triazene; purified recombinant sGC in presence of 3-ethyl-3-(ethylaminoethyl)-1-hydroxy-2-oxo-1-triazene
1.38
-
-
pulmonary artery homogenate after 2 h of treatment with NO
1.43
-
-
wild type enzyme, at pH 8.5 and 33C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol
2.96
-
-
in the presence of 0.1 mM 2,2-diethyl-1-nitroso-oxyhydrazine as NO source
3.25
-
-
sodium nitroprusside activated sGC activity
3.328
-
-
wild type enzyme, at pH 8.5 and 33C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol
3.402
-
-
wild type enzyme, at pH 8.5 and 33C, in the presence of 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol and 0.1 mM 5-[1-(phenylmethyl)-1H-indazol-3-yl]-2-furanmethanol
4.03
-
-
-
5.6
-
-
Fe(II)-unligated Gyc-88E(1-597)
8.5
-
-
with Mn2+
9.1
-
-
in the presence of 0.1 mM N-(beta-D-glucopyranosyl-N2-acetyl-S-nitroso-D,L-penicillaminamide)
12.1
-
-
in the presence of 0.1 mM N-(beta-D-glucopyranosyl-N2-acetyl-S-nitroso-D,L-penicillaminamide) and YC-1
15.7
-
-
with Mg2+
16.81
-
-
pH 7.4, 37C
17.5
-
-
-
22.1
-
-
NO-stimulated sGC
38
-
-
-
additional information
-
-
different values if assayed with Mg2+ or Mn2+
additional information
-
-
cGMP production in wild-type and sGCalpha1 KO mice
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
8.5
-
-
7.2
7.5
-
presence of activators
7.2
-
-
assay at
7.2
-
Q02846
assay at
7.2
-
-
assay at
7.2
-
-
assay at
7.4
7.5
Q81T60
assay at
7.4
7.6
-
-
7.4
-
-
-
7.4
-
-
assay at
7.4
-
-
assay at
7.4
-
Q90WX0, Q90WX1, Q90WX2
assay at; assay at; assay at
7.4
-
-
assay at
7.4
-
-
assay at
7.4
-
-
assay at
7.4
-
-
assay at
7.4
-
-
assay at
7.5
-
-
assay at
7.5
-
P18293
assay at
7.5
-
P16068, P19687
assay at; assay at
7.5
-
-
assay at
7.6
-
-
assay at
7.6
-
-, Q0PY32
assay at
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
22
-
Q81T60
assay at room temperature
30
-
-
assay at
30
-
Q02846
assay at
30
-
-
assay at
30
-
Q90WX0, Q90WX1, Q90WX2
assay at; assay at; assay at
30
-
-
assay at
30
-
-, Q0PY32
-
30
-
-
assay at
37
-
-
assay at
37
-
-
assay at
37
-
-
in vivo assay at
37
-
-
assay at
37
-
-
assay at
37
-
-
assay at
37
-
P18293
assay at
37
-
-
assay at
37
-
-
assay at
37
-
P16068, P19687
assay at; assay at
37
-
-
assay at
37
-
-
assay at
50
-
-
-
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
10
27
-
-
10
37
-
inclusion of 3-(5'-hydroxymethyl-3'-furyl)-1-benzylindazole moderately accelerates NO dissociation from sGC and beta2(1-217) at 37C and dramatically accelerates NO dissociation from sGC at 10C. Presence of guanosine-5'-[alpha,beta-methylene]triphosphate also dramatically accelerates NO dissociation from sGC at 10 C
10
60
-
-
15
25
-, Q2L6K7
;
20
35
-, Q0PY32
temperature profile, overview
additional information
-
-
L2 H-NOX is a mixture of 5- and 6-coordinate complexes under room temperature conditions, at low temperature, it is primarily 6-coordinate, and at high temperature, there is a shift toward the 5-coordinate geometry
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
brain natriuretic peptide stimulated membrane-bound GC
Manually annotated by BRENDA team
-
2-4 months old
Manually annotated by BRENDA team
-
soluble guanylyl cyclase sGC is mainly expressed during aggregation phase; upon development, mRNA expression of guanylyl cyclase GCA decreases about 5fold during aggregation and subsequently rises, reaching a maximum during the slug stage
Manually annotated by BRENDA team
Leishmania donovani MHOM/IN/1983/AG83
-
-
-
Manually annotated by BRENDA team
-
prominent for beta1 and to a lesser extent for the other subunits
Manually annotated by BRENDA team
-
high expression of beta1 and moderate to low expression of both alpha subunits
Manually annotated by BRENDA team
-
vascular wall and smooth muscle cells
Manually annotated by BRENDA team
-
primary aortic smooth muscle cells
Manually annotated by BRENDA team
-
human aortic smooth muscle cells
Manually annotated by BRENDA team
P19686, Q80WY4
;
Manually annotated by BRENDA team
-
pulmonary
Manually annotated by BRENDA team
-
pulmonary, cultured model
Manually annotated by BRENDA team
-
pulmonary, but not aorta
Manually annotated by BRENDA team
Q8SPV3
pulmonary
Manually annotated by BRENDA team
-
pulmonary
Manually annotated by BRENDA team
-, P16066, P20594, P25092, P51841
isozymes GC-A
Manually annotated by BRENDA team
-
isozymes GC-A
Manually annotated by BRENDA team
-
beta1-subunit, alpha2-subunit not present
Manually annotated by BRENDA team
-
alpha1 subunit most prominent
Manually annotated by BRENDA team
-
sGC beta1 is the most abundant and ubiquitous subunit
Manually annotated by BRENDA team
-
expression pattern of sGC in tissue form wild-type and spontaneously hypertensive rats, e.g. in rostral ventrolateral medulla, overview
Manually annotated by BRENDA team
-
mRNAs coding for all subunits high and homogeneously distributed
Manually annotated by BRENDA team
-
highest levels of mRNA for beta1, alpha1 and alpha2 subunits
Manually annotated by BRENDA team
-
atypical sGC primarily expressed
Manually annotated by BRENDA team
-
alpha2-subunit most prominent in the molecular layer followed by the ganglionic layer, low amounts in the granular layer
Manually annotated by BRENDA team
-
mRNA coding for beta1 and alpha2 subunits is high in Purkinje cells, small cells located close to the Purkinje cells, probably Bergmann glia, contain high densities of alpha1 mRNA. The granule cell layer is enriched in beta1 mRNA and shows moderate levels for alpha2 and low for alpha1 mRNA. The medial and lateral inferior olive nuclei show intense levels of beta1
Manually annotated by BRENDA team
-
alpha2 and beta1 abundant in Purkinje cells, small cells close to Purkinje cells express high levels of alpha1 mRNA, alpha2 and beta1 expression very high and homogeneous in the granule cell layer
Manually annotated by BRENDA team
-
present at weaker levels
Manually annotated by BRENDA team
-
all neocortical areas with high expression of all subunits, expression of the three subunits in all cortical layers, alpha1 subunit enriched in layer VI
Manually annotated by BRENDA team
-
beta1 subunit mRNA widely distributed through all cortical areas and cell layers with higher intensity in layer V, alpha2 mRNA homogeneously distributed through all cortical layers and alpha1 expression higher in layers IIIII and V
Manually annotated by BRENDA team
B2MVM0
co-expression of both alpha and beta subunits of NO-sensitive sGC
Manually annotated by BRENDA team
-
low expression of all subunit mRNAs in many of the white matter tracts
Manually annotated by BRENDA team
-
moderate expression of alpha1 and alpha2 mRNAs in many white matter tracts, beta1 mRNA expression is very low
Manually annotated by BRENDA team
-
the guanylyl cyclase messenger system is potentially responsive to hormones/neurotranmitters that may control the degree of relaxation in this vascular tissue
Manually annotated by BRENDA team
-
smooth muscle, low enzyme expression
Manually annotated by BRENDA team
Q3UWA6
guanylyl cyclase C is a protein that is expressed in the cells that line the normal intestine and by metastatic colorectal cancer cells. Isozyme GCC is universally expressed by primary and metastatic colorectal tumors
Manually annotated by BRENDA team
Mus musculus BALB/c
-
guanylyl cyclase C is a protein that is expressed in the cells that line the normal intestine and by metastatic colorectal cancer cells. Isozyme GCC is universally expressed by primary and metastatic colorectal tumors
-
Manually annotated by BRENDA team
-
purified sGC from lung
Manually annotated by BRENDA team
Mus musculus C57BL/6J
-
-
-
Manually annotated by BRENDA team
-, P16066, P20594, P25092, P51841
isozyme GC-B; isozyme GC-B
Manually annotated by BRENDA team
-, P16066, P20594, P25092, P51841
isozyme GC-C; isozyme GC-C
Manually annotated by BRENDA team
Mus musculus BALB/c
-
-
-
Manually annotated by BRENDA team
-
lower esophageal sphincter circular smooth muscle
Manually annotated by BRENDA team
-
retina and rod segements, retGC is a transmembrane protein localized in the outer segment
Manually annotated by BRENDA team
Q02846
retina and rod segements, retGC is a transmembrane protein localized in the outer segment
Manually annotated by BRENDA team
-
retina and rod segements, retGC is a transmembrane protein localized in the outer segment
Manually annotated by BRENDA team
Q90WX0, Q90WX1, Q90WX2
larval and adult eyes; larval and adult eyes; larval and adult eyes
Manually annotated by BRENDA team
-
anterior segment
Manually annotated by BRENDA team
-
; anterior segment
Manually annotated by BRENDA team
-
cardiac, tissue localization, overview
Manually annotated by BRENDA team
-, Q24LS5
eyestalk
Manually annotated by BRENDA team
-
dorsal root ganglion, NO-GC is restricted to non-neuronal cells in dorsal root ganglia
Manually annotated by BRENDA team
-
atrial- and C-type natriuretic peptide stimulated membrane-bound GC
Manually annotated by BRENDA team
-
smooth muscle and myenteric layer
Manually annotated by BRENDA team
-
from lean and obese rats, enzyme localization at left ventricle and coronary arteriole
Manually annotated by BRENDA team
-
at both tissue and single myocyte levels, sGC protein expression is heterogeneous, being high in sinoatrial node, right atrium, right ventricle and left ventricular subepicardium, but markedly reduced to absent in left atrium and left ventricular subendocardium
Manually annotated by BRENDA team
-, P16066, P20594, P25092, P51841
isozymes GC-A
Manually annotated by BRENDA team
-
isozymes GC-A
Manually annotated by BRENDA team
-
high levels of alpha2, and moderate levels of alpha1 and beta1 in the lateral amygdaloid nucleus. In the hippocampal formation the presence of alpha1 and alpha2 mRNA is higher in the dentate gyrus and in the presubiculum and lower in the different CA subfields, beta1 mRNA particularly enriched in the hippocampal fields. In the hilus, large neurons contain significantly higher levels of beta1 and alpha2
Manually annotated by BRENDA team
-, Q0PY32
low enzyme content
Manually annotated by BRENDA team
-
expression of the beta1 subunit moderate in the ventromedial, dorsomedial, arcuate, and geniculate nuclei. In these hypothalamic nuclei levels for the alpha subunits are low, all three subunits show moderate levels in the interpeduncular nucleus
Manually annotated by BRENDA team
Q02108, Q02153
;
Manually annotated by BRENDA team
-
mucosal cell
Manually annotated by BRENDA team
-, P16066, P20594, P25092, P51841
isozyme GC-C; isozyme GC-C; isozyme GC-C
Manually annotated by BRENDA team
-
isozymes GC-C and GC-G
Manually annotated by BRENDA team
Q3UWA6
guanylyl cyclase C is a protein that is expressed in the cells that line the normal intestine and by metastatic colorectal cancer cells
Manually annotated by BRENDA team
Mus musculus BALB/c
-
epithelium; guanylyl cyclase C is a protein that is expressed in the cells that line the normal intestine and by metastatic colorectal cancer cells
-
Manually annotated by BRENDA team
Q02108, Q02153
;
Manually annotated by BRENDA team
-
C-type natriuretic peptide stimulated membrane-bound-GC
Manually annotated by BRENDA team
-
isozyme GC-G
Manually annotated by BRENDA team
Q6TL19
epithelial cells of the proximal tubule and collecting ducts
Manually annotated by BRENDA team
-
in the anterior region expression of atypical sGCs in sensory neurons that innervate the dorsal and terminal organs, believed to be the main chemosensory organs of the larvae. Along the lateral body wall of larvae expressed by individual sensory neurons that express each of the subunits that appear to innervate basiconic sensilla and trachea. In the terminal segments, atypical sGCs are expressed in neurons that innervate peg sensilla on the caudal sensory cones
Manually annotated by BRENDA team
-
sGC alpha2 and beta1 subunits expressed strongly by dentate gyrus granule cells and CA1-CA3 pyramidal neurons, expression of alpha1 mRNA is high in CA2 pyramidal cells but low in the other CA subfields
Manually annotated by BRENDA team
-
expression of alpha1 and beta1 subunits of sGC
Manually annotated by BRENDA team
-
GC-A activity is detected on mucus secreting globlet cells, Clara cells and grat alveolar cells
Manually annotated by BRENDA team
-
isozyme GC-G
Manually annotated by BRENDA team
P19686, Q80WY4
;
Manually annotated by BRENDA team
P16068, P19687
;
Manually annotated by BRENDA team
Q3UWA6
C57BL/6-derived colorectal cancer cells
Manually annotated by BRENDA team
Mus musculus BALB/c
-
C57BL/6-derived colorectal cancer cells
-
Manually annotated by BRENDA team
-
alpha1 subunit mRNA with restricted distribution in mesencephalic areas, moderate to high levels in the dorsal aspects of the periaqueductal gray, vestibular lateral and medial nuclei, and in prepositus hypoglossal nuclei, low alpha1 and alpha2 mRNA in the dorsal raphe nucleus, pedunculopontine tegmental nucleus, and oculomotor nuclei. Expression for alpha subunits pronounced in pontine nuclei
Manually annotated by BRENDA team
-
high levels of beta1 but low levels of alpha subunits in superior colliculus, locus coeruleus presents high levels of beta1 and alpha1 subunits, ventral tegmental nucleus wiht moderate levels of expression for the three sGC subunits
Manually annotated by BRENDA team
-
guanylyl cyclase-C protein is expressed predominantly on the somata and dendrites of dopaminergic neurons in the the midbrain ventral tegmental area and substantia nigra compacta
Manually annotated by BRENDA team
-
brain- and atrial natriuretic peptide stimulated membrane-bound GC
Manually annotated by BRENDA team
-, Q2L6K7
expressed specifically in the AFD thermosensory neurons; expressed specifically in the AFD thermosensory neurons
Manually annotated by BRENDA team
-
present in the neurokinin 1 receptor-positive neurons in lamina I and many local circuit neurons in laminae I-II, expressed in most inhibitory interneurons
Manually annotated by BRENDA team
-
atypical sGC frequently co-localized in specific sensory neurons
Manually annotated by BRENDA team
-
no co-localization of sGC with phenylethanolamine N-methyltransferase or tyrosine hydroxylase, or NO synthase in neurons
Manually annotated by BRENDA team
Q9VEU5, Q9VEU6
the isozyme Gyc-89Da is encoded in neurons that are required for larval and adult ecdyses in the fruit fly; the isozyme Gyc-89Db is encoded in neurons that are required for larval and adult ecdyses in the fruit fly
Manually annotated by BRENDA team
-
midbrain dopamine neuron
Manually annotated by BRENDA team
-
high expression of beta1 and moderate to low expression of both alpha subunits
Manually annotated by BRENDA team
-
isozyme GC-D is exclusively expressed in the olfactory neuroepithelium in rodents
Manually annotated by BRENDA team
-
the membrane guanylate cyclase is exclusively expressed in the CO2-responsive olfactory sensory neurons
Manually annotated by BRENDA team
-
adrenal medulla derived cell line
Manually annotated by BRENDA team
-
very low expression of alpha1 and beta1 subunits of sGC
Manually annotated by BRENDA team
-
atypical sGC primarily expressed
Manually annotated by BRENDA team
Q90WX0, Q90WX1, Q90WX2
three membrane bound sensory guanylate cyclases, from developing and adult zebrafish retina; three membrane bound sensory guanylate cyclases, from developing and adult zebrafish retina; three membrane bound sensory guanylate cyclases, from developing and adult zebrafish retina
Manually annotated by BRENDA team
-, P16066, P20594, P25092, P51841
isozymes GC-E and GC-F are colocalized within the same photoreceptor cells of the retina; isozymes GC-E and GC-F are colocalized within the same photoreceptor cells of the retina; isozymes GC-E and GC-F are colocalized within the same photoreceptor cells of the retina
Manually annotated by BRENDA team
-
isozymes GC-E and GC-F are colocalized within the same photoreceptor cells of the retina
Manually annotated by BRENDA team
-
shows high levels of expression for beta1 mRNA and moderate for alpha1 and alpha2 mRNAs
Manually annotated by BRENDA team
-
anterior, sGC alpha2 inhibitory isoform and sGC alpha2 isoform are expressed
Manually annotated by BRENDA team
-
atrial natriuretic peptide stimulated membrane-bound GC
Manually annotated by BRENDA team
Leishmania donovani MHOM/IN/1983/AG83
-
-
-
Manually annotated by BRENDA team
Q963L5
sGC protein enriched at the leading edge of the cell during chemotaxis
Manually annotated by BRENDA team
-
soluble guanylate cyclase accounts for nearly 10% of the total guanylate cyclase activity of the retina
Manually annotated by BRENDA team
-
soluble guanylate cyclase accounts for nearly 20% of the total guanylate cyclase activity of the retina
Manually annotated by BRENDA team
-
present at high levels in inner retina but barely detectable in outer retina, ganglion cells exhibit moderate staining for sGC, strong immunostaining in subpopulations of bipolar and amacrine cells, weak staining in rod bipolar cells
Manually annotated by BRENDA team
Q90WX0, Q90WX1, Q90WX2
three membrane-bound sensory guanylate cyclases expressed in photoreceptor cells of the developing and adult zebrafish retina, expression analysis, overview; three membrane-bound sensory guanylate cyclases expressed in photoreceptor cells of the developing and adult zebrafish retina, expression analysis, overview; three membrane-bound sensory guanylate cyclases expressed in photoreceptor cells of the developing and adult zebrafish retina, expression analysis, overview
Manually annotated by BRENDA team
-, P16066, P20594, P25092, P51841
isozyme GC-E; isozyme GC-F; isozymes GC-E and GC-F are colocalized within the same photoreceptor cells of the retina
Manually annotated by BRENDA team
-
isozymes GC-E and GC-F are colocalized within the same photoreceptor cells of the retina
Manually annotated by BRENDA team
-
type 1 membrane-bound guanylate cyclase is expressed exclusively in the retina
Manually annotated by BRENDA team
P16067, P18910
natriuretic peptide-activated guanylate cyclase NPR-A is expressed in several layers of the retina including amacrine cells and the ganglion cell layer; natriuretic peptide-activated guanylate cyclase NPR-B is expressed in several layers of the retina including amacrine cells and the ganglion cell layer
Manually annotated by BRENDA team
Mus musculus C57BL/6J
-
-
-
Manually annotated by BRENDA team
Mus musculus C57BL/6J
-
-
-
Manually annotated by BRENDA team
-, Q0PY32
-
Manually annotated by BRENDA team
-
upon development, mRNA expression of guanylyl cyclase GCA decreases about 5fold during aggregation and subsequently rises, reaching a maximum during the slug stage
Manually annotated by BRENDA team
-
upper villous and crypt cells
Manually annotated by BRENDA team
-
protein expression of the alpha1- and beta1-sGC isoforms is significantly lower in the adult compared with the newborn bronchial tissue
Manually annotated by BRENDA team
-
tracheal and lung tissue smooth muscle
Manually annotated by BRENDA team
-
pulmonary artery smooth muscle layer
Manually annotated by BRENDA team
-
lower esophageal sphincter circular smooth muscle
Manually annotated by BRENDA team
-
gastrointestinal smooth muscle
Manually annotated by BRENDA team
-
gastrointestinal tract, muscularis mucosae and vasculature
Manually annotated by BRENDA team
Q02108, Q02153
gastrointestinal; gastrointestinal
Manually annotated by BRENDA team
-
primary aortic smooth muscle cells
Manually annotated by BRENDA team
-
predominantly expressed from round spermatids to spermatozoa in mouse testis at both the mRNA and protein levels
Manually annotated by BRENDA team
-
isozyme GC-G
Manually annotated by BRENDA team
-
present at weaker levels
Manually annotated by BRENDA team
-
NO-GC is distinctly expressed in neurons of the mouse dorsal horn
Manually annotated by BRENDA team
-
gastrointestinal smooth muscle
Manually annotated by BRENDA team
-
smooth muscle, low enzyme expression
Manually annotated by BRENDA team
-
shows high expression of beta1, moderate of alpha2, and low of alpha1
Manually annotated by BRENDA team
-
moderate expression of beta1, alpha subunits expression is very low
Manually annotated by BRENDA team
-
atrial natriuretic peptide stimulated membrane-bound GC and guanylin-stimulated GC-C
Manually annotated by BRENDA team
-
colon cancer cell line
Manually annotated by BRENDA team
-
detectable at day 18 of postnatal development and thereafter increasing progressively
Manually annotated by BRENDA team
-
many of the thalamic nuclei contain the three subunits, alpha1 and beta1 mRNA in both the anterior and posterior ventralis nuclei and in the pulvinar and reticular nuclei, alpha2 subunit mRNA is present at low levels in the pulvinar nucleus and barely detectable in other thalamic nuclei
Manually annotated by BRENDA team
-
high levels of mRNA for the three subunits in the medial habenula, in other thalamic nuclei mainly beta1 mRNA
Manually annotated by BRENDA team
-
GC-B is the predominant functional membrane-bound guanylyl cyclase subtype
Manually annotated by BRENDA team
-, Q24LS5
abundance of the CsCG-YO1 transcript in Y-organs during a molt cycle: the level of CsGC-YO1 in Y-organs is elevated during intermolt, C4, and lower during premolt stages D1-D3; CsGC-YO1 transcript abundance during a molt cycle, overview
Manually annotated by BRENDA team
additional information
-
newborn aged 25 days
Manually annotated by BRENDA team
additional information
-
brainstem, subnucleus caudalis, where it is present in neuronal cell bodies in the superficial laminae and in astrocytes in deeper lamina
Manually annotated by BRENDA team
additional information
-
in Purkinje cell somata alpha2- and beta1-subunit present, alpha2-subunit not present in white matter
Manually annotated by BRENDA team
additional information
-
not detectable in spermatogonia, Sertoli cells, and spermatocytes
Manually annotated by BRENDA team
additional information
-
in the superficial dorsal horn present throughout the gray matter, whereas unmyelinated primary afferent fibers terminating in the superficial dorsal horn lack sGC
Manually annotated by BRENDA team
additional information
-
no alpha subunits in the substantia nigra
Manually annotated by BRENDA team
additional information
-
in cortex present at weaker levels, whereas photoreceptors, horizontal cells, Mller cells, and AII amacrine cells are immunonegative
Manually annotated by BRENDA team
additional information
-
atypical sGC expressed throughout development
Manually annotated by BRENDA team
additional information
-
T84 cell and SW480 cell
Manually annotated by BRENDA team
additional information
-
no expression of alpha1 and beta1 subunits of sGC in DU-145 cells
Manually annotated by BRENDA team
additional information
-
heart tissue expression pattern, overview
Manually annotated by BRENDA team
additional information
-
GC-G has a broad tissue distribution in rodents
Manually annotated by BRENDA team
additional information
-, Q0PY32
tissue distribution, overview
Manually annotated by BRENDA team
additional information
-
analysis of sGC in gastrointestinal tissue, immunohistochemic analysis
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Tetrahymena thermophila B2086
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Manually annotated by BRENDA team
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content of alpha2-subunit in Purkinje cell somata is low
Manually annotated by BRENDA team
P16068, P19687
;
Manually annotated by BRENDA team
Leishmania donovani MHOM/IN/1983/AG83
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-
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Manually annotated by BRENDA team
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glomerular and papillary membranes
Manually annotated by BRENDA team
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alpha2-subunit, in Purkinje cell somata
Manually annotated by BRENDA team
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at the apical membrane GCA and GCC both present, at the basolateral membrane only GCA present, GCC is delivered directly to the apical membrane
Manually annotated by BRENDA team
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guanylate cyclase C is a transmembrane enzyme
Manually annotated by BRENDA team
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retGC is a transmembrane protein localized in the outer segment
Manually annotated by BRENDA team
Q02846
retGC is a transmembrane protein localized in the outer segment
Manually annotated by BRENDA team
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retGC is a transmembrane protein localized in the outer segment
Manually annotated by BRENDA team
Q90WX0, Q90WX1, Q90WX2
three membrane-bound sensory guanylate cyclases in retina; three membrane-bound sensory guanylate cyclases in retina; three membrane-bound sensory guanylate cyclases in retina
Manually annotated by BRENDA team
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rod outer segment membrane
Manually annotated by BRENDA team
-, Q24LS5
CsGC-YO1 is a membrane-associated protein
Manually annotated by BRENDA team
P18293
transmembrane isozyme GC-A
Manually annotated by BRENDA team
Q8TA72
membrane-bound guanylate cyclase
Manually annotated by BRENDA team
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membrane-bound guanylate cyclase
Manually annotated by BRENDA team
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GC-A is a single transmembrane spanning protein
Manually annotated by BRENDA team
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nuclear localization of the soluble guanylate cyclase in alveolar macrophages in nucleoplasm
Manually annotated by BRENDA team
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overlying the acrosome and midpiece of the flagellum in mature sperm
Manually annotated by BRENDA team
-, P16066, P20594, P25092, P51841
; membrane guanylyl cyclase receptor isozyme GC-A; membrane guanylyl cyclase receptor isozyme GC-B; membrane guanylyl cyclase receptor isozyme GC-C; membrane guanylyl cyclase receptor isozymes GC-D, GC-E, to GC-G
Manually annotated by BRENDA team
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several membrane guanylyl cyclase receptor isozymes, GC-A to GC-G
Manually annotated by BRENDA team
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cytoplasm and nucleoplasm
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Manually annotated by BRENDA team
Q5YLC2
soluble guanylate cyclase
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Manually annotated by BRENDA team