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Information on EC 4.6.1.16 - tRNA-intron lyase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9M1E8

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IUBMB Comments
The enzyme catalyses the final stage in the maturation of tRNA molecules.
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Select one or more organisms in this record: ?
This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q9M1E8
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Eukaryota, Archaea, Bacteria
Reaction Schemes
pretRNA
=
a 3'-half-tRNA molecule with a 5'-OH end
+
a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end
+
an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
Synonyms
homing endonuclease, i-tevi, splicing endonuclease, trna splicing endonuclease, hspc117, intron-encoded endonuclease, hclp1, trna-splicing endonuclease, af endonuclease, rna splicing endonuclease, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nuclease, transfer ribonucleate intron endoribo-
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splicing endonuclease
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transfer ribonucleate intron endoribonuclease
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transfer splicing endonuclease
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tRNA intron endonuclease
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tRNA splicing endonuclease
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tRNA-intron endonuclease
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tRNATRPintron endonuclease
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
show the reaction diagram
structural requirements of substrate
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
endoribonuclease reaction
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PATHWAY SOURCE
PATHWAYS
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-, -
SYSTEMATIC NAME
IUBMB Comments
pretRNA lyase (intron-removing; cyclic-2',3'-phosphate-forming)
The enzyme catalyses the final stage in the maturation of tRNA molecules.
CAS REGISTRY NUMBER
COMMENTARY hide
117444-13-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
tRNA precursor
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
show the reaction diagram
-
-
-
?
tRNA precursor
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
structural requirements of substrate
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SEN21_ARATH
237
0
27359
Swiss-Prot
other Location (Reliability: 5)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
two genes coding for different subunits
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Akama, K.; Junker, V.; Beier, H.
Identification of two catalytic subunits of tRNA splicing endonuclease from Arabidopsis thaliana
Gene
257
177-185
2000
Arabidopsis thaliana (Q9M1E8), Arabidopsis thaliana
Manually annotated by BRENDA team
Akama, K.; Junker, V.; Yukawa, Y.; Sugiura, M.; Beier, H.
Splicing of arabidopsis tRNA(Met) precursors in tobacco cell and wheat germ extracts
Plant Mol. Biol.
44
155-165
2000
Arabidopsis thaliana, Triticum aestivum
Manually annotated by BRENDA team