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Information on EC 4.4.1.9 - L-3-cyanoalanine synthase and Organism(s) Solanum tuberosum and UniProt Accession Q76MX2

for references in articles please use BRENDA:EC4.4.1.9
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IUBMB Comments
Contains pyridoxal phospate.
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This record set is specific for:
Solanum tuberosum
UNIPROT: Q76MX2
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Word Map
The taxonomic range for the selected organisms is: Solanum tuberosum
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
beta-cas, beta-cyanoalanine synthase, o-acetyl-l-serine sulfhydrylase, cys-c1, cyanoalanine synthase, atcysc1, beta-cyano-l-alanine synthase, pcas-1, bsas3, l-3-cyanoalanine synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
beta-cyano-L-alanine synthase
-
-
-
-
beta-cyanoalanine synthase
synthase,.beta.-cyanoalanine
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
L-cysteine hydrogen-sulfide-lyase (adding hydrogen cyanide; L-3-cyanoalanine-forming)
Contains pyridoxal phospate.
CAS REGISTRY NUMBER
COMMENTARY hide
9059-53-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-cysteine + NaCN
L-3-cyanoalanin + Na2S
show the reaction diagram
-
-
-
?
L-cysteine + HCN
H2S + L-3-cyanoalanine
show the reaction diagram
-
-
-
-
ir
L-cysteine + HCN
sulfide + L-3-cyanoalanine
show the reaction diagram
L-cysteine + NaCN
L-3-cyanoalanin + Na2S
show the reaction diagram
-
-
-
?
O-acetyl-L-serine + sulfide
L-cysteine + acetate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-cysteine + HCN
sulfide + L-3-cyanoalanine
show the reaction diagram
O-acetyl-L-serine + sulfide
L-cysteine + acetate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
salicylic acid
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-aminocyclopropane-1-carboxylic acid
-
stimulates
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.13 - 0.235
CN-
2.76 - 2.82
L-cysteine
1.5 - 2
Na2S
pH 7.5
0.134 - 2.14
O-acetyl-L-serine
9.51
Sulfide
-
pH 8.5
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
49.5
substrate O-acetyl-L-serine
0.25
substrate L-cysteine
0.43
substrate O-acetyl-L-serine
66.2
substrate L-cysteine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cv. Danshaku
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
6.2 times and 2.5 times higher levels than in tubers and leaves, respectively
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CAS1_SOLTU
351
0
38201
Swiss-Prot
Mitochondrion (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37600
x * 37600, immunoblot
38200
x * 38200, immunoblot
37000
-
2 * 37000, SDS-PAGE
54000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 37000, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
80% acetone, ammonium sulfate, DEAE Sephacel, Mono Q, Resource PHE, preparative electrophoresis, TSK gel 3000
-
recombinant PCAS-1
recombinant PCAS-2
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of PCAS-1 in Escherichia coli
expression of PCAS-2 in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wen, J.Q.; Huang, G.; Liang, W.S.; Liang, H.G.
Increase of HCN and beta-cyanoalanine synthase activity during ageing of potato tuber slices
Plant Sci.
125
147-151
1997
Solanum tuberosum
-
Manually annotated by BRENDA team
Maruyama, A.; Saito, K.; Ishizawa, K.
beta-Cyanoalanine synthase and cysteine synthase from potato: molecular cloning, biochemical characterization, and spatial and hormonal regulation
Plant Mol. Biol.
46
749-760
2001
Solanum tuberosum (Q76MX2), Solanum tuberosum (Q9FS29), Solanum tuberosum
Manually annotated by BRENDA team
Maruyama, A.; Ishizawa, K.; Takagi, T.
Purification and characterization of beta-cyanoalanine synthase and cysteine synthases from potato tubers: are beta-cyanoalanine synthase and mitochondrial cysteine synthase same enzyme?
Plant Cell Physiol.
41
200-208
2000
Solanum tuberosum
Manually annotated by BRENDA team