Information on EC 4.4.1.6 - S-alkylcysteine lyase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
4.4.1.6
-
RECOMMENDED NAME
GeneOntology No.
S-alkylcysteine lyase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an S-alkyl-L-cysteine + H2O = an alkyl thiol + NH3 + pyruvate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
-
-
alpha,beta elimination; alpha,beta elimination of H2S or RSH, C-S bond cleavage; of H2S or RSH, C-S bond cleavage
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SYSTEMATIC NAME
IUBMB Comments
S-alkyl-L-cysteine alkyl-thiol-lyase (deaminating; pyruvate-forming)
A pyridoxal-phosphate protein. The enzyme cleaves a carbon-sulfur bond, releasing an alkyl thiol and an unstable enamine product that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form pyruvate and ammonia. The latter reaction, which can occur spontaneously, can also be catalysed by EC 3.5.99.10, 2-iminobutanoate/2-iminopropanoate deaminase. Possibly identical, in yeast, with EC 4.4.1.8 cystathionine beta-lyase.
CAS REGISTRY NUMBER
COMMENTARY hide
62213-27-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
41A
-
-
Manually annotated by BRENDA team
41A
-
-
Manually annotated by BRENDA team
S-methyl-L-cysteine-adapted
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
benzyl-L-cysteine
?
show the reaction diagram
-
-
-
?
L-cysteine-S-sulphate
?
show the reaction diagram
-
-
-
?
L-cystine
?
show the reaction diagram
-
-
-
?
L-djenkolate
?
show the reaction diagram
-
-
-
-
-
L-djenkolate
pyruvate + NH3 + S-(mercaptomethyl)cysteine
show the reaction diagram
L-djenkolate sulphoxide
?
show the reaction diagram
-
-
-
?
L-Selenodjenkolate
Se-(hydroselenomethyl)selenocysteine + pyruvate + NH3
show the reaction diagram
O-methyl-DL-serine
?
show the reaction diagram
-
-
-
?
S-carbamoyl-L-cysteine
?
show the reaction diagram
-
-
-
?
S-methyl-L-cysteine
methyl mercaptane + pyruvate + NH3
show the reaction diagram
S-methyl-L-cysteine sulfoxide
methyl methanethiolsulfinate + pyruvate + NH3
show the reaction diagram
S-propyl-L-cysteine
?
show the reaction diagram
-
-
-
?
Se-(hydroselenomethyl)selenocysteine
bis(hydroseleno)methane + pyruvate + NH3
show the reaction diagram
-
-
-
ir
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-djenkolate
?
show the reaction diagram
-
-
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
HgCl2
-
57% inhibition at 1 mM
hydoxylamine
-
complete inhibition at 1 mM
p-chloromercuribenzoate
-
82% inhibition at 1 mM
phenylhydrazine
-
complete inhibition at 1 mM
S-methyl-L-cysteine
-
substrate inhibition, when concentration exceeds 10 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.8
L-cysteine
-
-
0.3 - 3.6
L-djenkolate
2
L-selenodjenkolate
-
-
6.5
O-methyl-DL-serine
-
-
0.008
pyridoxal phosphate
-
-
0.73
S-Benzyl-L-cysteine
-
-
2.3 - 5.3
S-methyl-L-cysteine
2.3
S-methyl-L-cysteine sulfoxide
-
-
4.6
Se-methyl-L-selenocysteine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9.5
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
hypocotyl of 5 day old seedlings
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
39000
-
2 * 39000, SDS-PAGE
42000
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x * 42000, SDS-PAGE
76000
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gel filtration
144000
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ultracentrifugal analysis
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
polymer
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x * 42000, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
no loss of activity after repeated thawing and freezing
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, 5 months
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-20°C, 10 mM potassium phospate buffer, pH 7.2, with 50% glycerol, 0.01 mM pyridoxal 5'-phosphate and 0.01% 2-mercaptoethanol, 1 month without loss of activity
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-20°C, almost all activity lost after two weeks
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