Information on EC 4.4.1.32 - C-phycocyanin alpha-cysteine-84 phycocyanobilin lyase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.4.1.32
-
RECOMMENDED NAME
GeneOntology No.
C-phycocyanin alpha-cysteine-84 phycocyanobilin lyase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[C-phycocyanin alpha-subunit]-Cys84-phycocyanobilin = apo-[C-phycocyanin alpha-subunit] + (2R,3E)-phycocyanobilin
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
[C-phycocyanin alpha-subunit]-Cys84-phycocyanobilin:(2R,3E)-phycocyanobilin lyase
The enzyme, characterized from the cyanobacterium Synechococcus elongatus PCC 7942, catalyses the covalent attachment of the phycobilin chromophore phycocyanobilin to cysteine 84 of the alpha subunit of the phycobiliprotein C-phycocyanin.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Q44115: subunit alpha (cpcE), Q44116: phycobilisome maturation protein (cpcF)
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
apo-[C-phycocyanin alpha-subunit] + (2R,3E)-phycocyanobilin
[C-phycocyanin alpha-subunit]-Cys84-phycocyanobilin
show the reaction diagram
[C-phycocyanin alpha-subunit] + phycocyanobilin
[C-phycocyanin alpha-subunit]-Cys84-phycocyanobilin
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
apo-[C-phycocyanin alpha-subunit] + (2R,3E)-phycocyanobilin
[C-phycocyanin alpha-subunit]-Cys84-phycocyanobilin
show the reaction diagram
-
-
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
phycoerythrobilin
-
competitive inhibitor of (2R,3E)-phycocyanobilin addition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000024
(2R,3E)-phycocyanobilin
-
pH 8.0, 37°C
0.0108
apo-[C-phycocyanin alpha-subunit]
-
pH 8.0, 37°C
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.025
(2R,3E)-phycocyanobilin
Synechococcus elongatus
-
pH 8.0, 37°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00086
phycoerythrobilin
-
pH 8.0, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22000
-
1 * 29000 (CpcE) + 1 * 22000 (CpcF), SDS-PAGE; CpcE and CpcF form an enzymatically active 1:1 complex
29000
-
1 * 29000 (CpcE) + 1 * 22000 (CpcF), SDS-PAGE; CpcE and CpcF form an enzymatically active 1:1 complex
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
1 * 29000 (CpcE) + 1 * 22000 (CpcF), SDS-PAGE; CpcE and CpcF form an enzymatically active 1:1 complex
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant CpcE and CpcF proteins
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
overexpression in Escherichia coli
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis