Information on EC 4.4.1.3 - dimethylpropiothetin dethiomethylase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
4.4.1.3
-
RECOMMENDED NAME
GeneOntology No.
dimethylpropiothetin dethiomethylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S,S-dimethyl-beta-propiothetin = dimethyl sulfide + acrylate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
-
-
of RSH, C-S bond cleavage
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
dimethylsulfoniopropanoate degradation I (cleavage)
-
-
Sulfur metabolism
-
-
superpathway of dimethylsulfoniopropanoate degradation
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-
SYSTEMATIC NAME
IUBMB Comments
S,S-dimethyl-beta-propiothetin dimethyl-sulfide-lyase (acrylate-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
9026-85-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
induction of enzyme by beta-dimethylsulfonioproprionate, acrylate or beta-hydroxyproprionate
-
-
Manually annotated by BRENDA team
strain M3A
-
-
Manually annotated by BRENDA team
strain M3A
-
-
Manually annotated by BRENDA team
Alexandrium ssp.
Halim
-
-
Manually annotated by BRENDA team
Alexandrium ssp. Halim
Halim
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
gene dddL
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
high enzymic activity
-
-
Manually annotated by BRENDA team
no activity in Gephyrocapsa oceanica
-
-
-
Manually annotated by BRENDA team
no activity in Heterosigma akashiwo
-
-
-
Manually annotated by BRENDA team
no activity in Isochrysis galbana
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain 2.4.1, no activity in strain ATCC 17025, gene dddL
-
-
Manually annotated by BRENDA team
gene DMSP
-
-
Manually annotated by BRENDA team
high enzymic activity
-
-
Manually annotated by BRENDA team
gene dddL
-
-
Manually annotated by BRENDA team
gene dddL
-
-
Manually annotated by BRENDA team
3 isoform: UMDL1, UMDL2, UMDL3
-
-
Manually annotated by BRENDA team
alpha-subclass, enzyme is induced by beta-dimethylsulfoniopropanoate, acrylate, beta-hydroxyproprionate
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
beta-dimethylsulfoniopropanoate
dimethylsulfide + acrylate
show the reaction diagram
dimethylseleniopropionate
dimethylselenide + acrylate
show the reaction diagram
dimethylsulfoniopropionate
dimethylsulfide + acrylate
show the reaction diagram
S,S-dimethyl-beta-propiothetin
dimethyl sulfide + acrylate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S,S-dimethyl-beta-propiothetin
dimethyl sulfide + acrylate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
none of the metals Co, Cu, Mn, Ni or Zn is detected at above background levels
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,4-dinitrophenol
-
-
azide
dimethylseleniopropionate
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membrane-impermeable thiol-binding reagents
-
-
-
Nystatin
-
-
p-Chloromercuriphenylsulfonate
-
-
additional information
-
not inhibitory: EDTA at 25 mM, bipyridyl at 2.5 mM
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
light
-
Polyamines
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sulfhydryl compounds
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required
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
14
beta-dimethylsulfoniopropanoate
-
pH 6.0, 30°C
0.02 - 2
dimethylsulfoniopropionate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
assay methods for crude cells and in vivo method for whole cells
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 10
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activity is independent of the pH between pH 4 and pH 10
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 10
-
activity is independent of the pH between pH 4 and pH 10
5 - 7
-
more than 70% of maximum activity
6.5 - 9
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pH 6.5: about 20% of maximal activity, pH 9.0: about 40% loss of activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37 - 40
-
-
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Ruegeria lacuscaerulensis (strain DSM 11314 / KCTC 2953 / ITI-1157)
Ruegeria lacuscaerulensis (strain DSM 11314 / KCTC 2953 / ITI-1157)
Ruegeria lacuscaerulensis (strain DSM 11314 / KCTC 2953 / ITI-1157)
Ruegeria lacuscaerulensis (strain DSM 11314 / KCTC 2953 / ITI-1157)
Ruegeria lacuscaerulensis (strain DSM 11314 / KCTC 2953 / ITI-1157)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48000
-
gel filtration
49972
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2 * 49972, calculated, 2 * 50000, SDS-PAGE
50000
-
2 * 49972, calculated, 2 * 50000, SDS-PAGE
78000
-
1 * 78000, SDS-PAGE
95300
-
sedimentation equilibrium ultracentrifugation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 49972, calculated, 2 * 50000, SDS-PAGE
monomer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
rapid loss of activity above
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 50 mM MES, pH 6, no detectable loss of activity over a period of several weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant protein
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
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expression in Escherichia coli
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gene dddL, expression analysis
gene dddl, expression analysis, expression in Escherichia coli
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gene dddL, genetic organization and expression analysis, expression in Escherichia coli
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gene DMSP, quantitative expression analysis in Roseobacter denitrificans solo-culture and in Roseobacter denitrificans-Salinispora arenicola dual co-culture
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
in a Roseobacter denitrificans-Salinispora arenicola dual co-culture, the Roseobacter denitrificans DMSP genes exhibits a large 2.7fold increase of gene expression compared to Roseobacter denitrificans solo-culture with a more rapid onset after initial mixing of the two bacteria
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
environmental protection
D295A
-
complete loss of activity
D297A
-
complete loss of activity
D307A
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complete loss of activity
E406A
-
complete loss of activity
E421A
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complete loss of activity
H371A
-
complete loss of activity
additional information
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Sulfitobacter J443, DddL-mutant has lost its Ddd+ phenotype, showing that this species has no other gene that can substitute for dddL, the wild type produces 385 nmol dimethylsulfide, the mutant produces only 0.7 nmol dimethylsulfide