Information on EC 4.4.1.24 - (2R)-sulfolactate sulfo-lyase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.4.1.24
-
RECOMMENDED NAME
GeneOntology No.
(2R)-sulfolactate sulfo-lyase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2R)-3-sulfolactate = pyruvate + HSO3-
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
(R)-cysteate degradation
-
-
Cysteine and methionine metabolism
-
-
sulfolactate degradation I
-
-
SYSTEMATIC NAME
IUBMB Comments
(2R)-3-sulfolactate bisulfite-lyase (pyruvate-forming)
Requires iron(II). This inducible enzyme participates in cysteate degradation by the bacterium Paracoccus pantotrophus NKNCYSA and in 3-sulfolactate degradation by the bacterium Chromohalobacter salexigens. The enzyme is specific for the (R) isomer of its substrate.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strongly inducible enzyme, gene suyAB
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2R)-3-sulfolactate
pyruvate + bisulfite
show the reaction diagram
3-sulfolactate
pyruvate + bisulfite
show the reaction diagram
additional information
?
-
activity with 3-sulfopyruvate in vivo by cysteate-grown cells only after membrane permeabilization
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-sulfolactate
pyruvate + bisulfite
show the reaction diagram
Q58Y44
desulfonation step involved in cysteate dissimilation, metabolic pathway in the cell, overview
-
-
?
additional information
?
-
Q58Y44
activity with 3-sulfopyruvate in vivo by cysteate-grown cells only after membrane permeabilization
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
can substitute for Fe2+ at 2mM
Fe2+
required for activity
Mn2+
can substitute for Fe2+ at 2mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
70% inhibition at 1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.016
-
crude extract
0.13
-
crude extract
0.2
-
in the soluble fraction
0.3
purified enzyme
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
i.e. 2-amino-3-sulfopropionate, is the sole carbon and energy source of the organism with either nitrate or molecular oxygen as terminal electron acceptor
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8000
x * 8000, alpha-subunit, + x * 42000, beta-subunit, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 8000, alpha-subunit, + x * 42000, beta-subunit, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
after freezing no residual enzyme activity
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 1 day, 50% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
from cysteate-grown cells by anion exchange chromatography and hydrophobic interaction chromatography, to near homogeneity
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene suyAB, DNA and amino acid sequence determination and analysis