Information on EC 4.4.1.24 - (2R)-sulfolactate sulfo-lyase

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The expected taxonomic range for this enzyme is: Proteobacteria

EC NUMBER
COMMENTARY
4.4.1.24
-
RECOMMENDED NAME
GeneOntology No.
(2R)-sulfolactate sulfo-lyase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(2R)-3-sulfolactate = pyruvate + bisulfite
show the reaction diagram
-
-
-
-
(2R)-3-sulfolactate = pyruvate + bisulfite
show the reaction diagram
putative reaction mechanism
-, Q58Y44
PATHWAY
KEGG Link
MetaCyc Link
(R)-cysteate degradation
-
Cysteine and methionine metabolism
-
sulfolactate degradation I
-
SYSTEMATIC NAME
IUBMB Comments
(2R)-3-sulfolactate bisulfite-lyase (pyruvate-forming)
Requires iron(II). This inducible enzyme participates in cysteate degradation in Paracoccus pantotrophus NKNCYSA and in 3-sulfolactate degradation in Chromohalobacter salexigens. The enzyme is specific for the (R) isomer.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
(R)-sulfolactate sulfo-lyase
-
-
3-sulfolactate sulfo-lyase
Q58Y44
-
desulfonative sulfolactate sulfo-lyase
-
-
desulfonative sulfolactate sulfo-lyase
-
-
-
additional information
Q58Y44
the enzyme belongs to the altronate dehydratase family
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strongly inducible enzyme, gene suyAB
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2R)-3-sulfolactate
pyruvate + bisulfite
show the reaction diagram
-
-
-
-
?
(2R)-3-sulfolactate
pyruvate + bisulfite
show the reaction diagram
-
-
-
-
?
(2R)-3-sulfolactate
pyruvate + bisulfite
show the reaction diagram
-
-
-
-
?
3-sulfolactate
pyruvate + bisulfite
show the reaction diagram
-, Q58Y44
-
-
-
?
3-sulfolactate
pyruvate + bisulfite
show the reaction diagram
-, Q58Y44
desulfonation step involved in cysteate dissimilation, metabolic pathway in the cell, overview
-
-
?
additional information
?
-
-, Q58Y44
activity with 3-sulfopyruvate in vivo by cysteate-grown cells only after membrane permeabilization
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-sulfolactate
pyruvate + bisulfite
show the reaction diagram
-, Q58Y44
desulfonation step involved in cysteate dissimilation, metabolic pathway in the cell, overview
-
-
?
additional information
?
-
-, Q58Y44
activity with 3-sulfopyruvate in vivo by cysteate-grown cells only after membrane permeabilization
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Co2+
-, Q58Y44
can substitute for Fe2+ at 2mM
Fe2+
-, Q58Y44
required for activity
Mn2+
-, Q58Y44
can substitute for Fe2+ at 2mM
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
EDTA
-, Q58Y44
70% inhibition at 1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
-, Q58Y44
the enzyme is strongly inducible
-
additional information
-
inducible enzyme activity, activity detected in extract of sulfolactate-grown cells
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.016
-
-
crude extract
0.13
-
-
crude extract
0.2
-
-
in the soluble fraction
0.3
-
-, Q58Y44
purified enzyme
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-, Q58Y44
i.e. 2-amino-3-sulfopropionate, is the sole carbon and energy source of the organism with either nitrate or molecular oxygen as terminal electron acceptor
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
42000
-
-
SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-, Q58Y44
x * 8000, alpha-subunit, + x * 42000, beta-subunit, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
after freezing no residual enzyme activity
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4°C, 1 day, 50% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
from cysteate-grown cells by anion exchange chromatography and hydrophobic interaction chromatography, to near homogeneity
-, Q58Y44
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
gene suyAB, DNA and amino acid sequence determination and analysis
-, Q58Y44