Information on EC 4.4.1.19 - phosphosulfolactate synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Search Reference ID:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
4.4.1.19
-
RECOMMENDED NAME
GeneOntology No.
phosphosulfolactate synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2R)-2-O-phospho-3-sulfolactate = phosphoenolpyruvate + HSO3-
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
addition
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
coenzyme M biosynthesis
-
-
coenzyme M biosynthesis I
-
-
Methane metabolism
-
-
Microbial metabolism in diverse environments
-
-
SYSTEMATIC NAME
IUBMB Comments
(2R)-2-O-phospho-3-sulfolactate hydrogen-sulfite-lyase (phosphoenolpyruvate-forming)
Requires Mg2+. The enzyme from Methanococcus jannaschii catalyses the Michael addition of sulfite to phosphoenolpyruvate. It specifically requires phosphoenolpyruvate and its broad alkaline pH optimum suggests that it uses sulfite rather than bisulfite.
CAS REGISTRY NUMBER
COMMENTARY hide
473575-53-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
bisulfite + phosphoenolpyruvate
(2R)-O-phospho-3-sulfolactate
show the reaction diagram
-
-
enzyme is involved in the biosynthesis of sulfoquinovosyl diacylglycerol, one of the major sulfur-containing glycolipids in the chloroplast thylakoid membrane
-
r
sulfite + phosphoenolpyruvate
(2R)-O-phospho-3-sulfolactate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
sulfite + phosphoenolpyruvate
(2R)-O-phospho-3-sulfolactate
show the reaction diagram
Q57703
-
-
-
-
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
MgCl2
inhibition above 10 mM
phosphoenolpyruvate
substrate inhibition above 5 mM
sulfite
substrate inhibition above 3 mM
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
9.8
phosphoenolpyruvate
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.9
phosphoenolpyruvate
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Bacillus subtilis (strain 168)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
trimer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 20 mM Tris-HCl, 1 mM MgCl2, pH 8.0, 2 months, no loss of activity
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K137N
no measurable enzymatic activity
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
5 mM MgCl2 restores activity of EDTA-treated enzyme
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
-
selenium contamination is one of the most serious problems in agriculture in Se-rich regions, cpSL transgenic plants can be used for Se phytoremediation under field conditions, Selenium accumulation is significantly enhanced in the shoots of the cpSL transgenic plants compared to wild type, cpSL plants are more tolerant to the toxic sediment-soil than the wild type
Show AA Sequence (138 entries)
Please use the Sequence Search for a certain query.