Information on EC 4.4.1.19 - phosphosulfolactate synthase

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The expected taxonomic range for this enzyme is: Eukaryota, Archaea

EC NUMBER
COMMENTARY
4.4.1.19
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RECOMMENDED NAME
GeneOntology No.
phosphosulfolactate synthase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(2R)-2-O-phospho-3-sulfolactate = phosphoenolpyruvate + HSO3-
show the reaction diagram
; The reaction has been observed only in the direction of Michael addition of sulfite to phosphoenolpyruvate. It is the first reaction of biosynthesis of coenzyme M in the methanogenic archaeon Methanococcus jannaschii. Its broad alkaline pH optimum suggests that it uses sulfite rather than bisulfite
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(2R)-2-O-phospho-3-sulfolactate = phosphoenolpyruvate + HSO3-
show the reaction diagram
catalyses the Michael addition of sulfite to phosphoenolpyruvate, requires Mg2+, specifically requires phosphoenolpyruvate and its broad alkaline pH optimum suggests that it uses sulfite rather than bisulfite
(2R)-2-O-phospho-3-sulfolactate = phosphoenolpyruvate + HSO3-
show the reaction diagram
mechanism, enzyme is not a member of the enolase superfamily
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
addition
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
coenzyme M biosynthesis I
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coenzyme M biosynthesis
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Methane metabolism
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
(2R)-2-O-phospho-3-sulfolactate hydrogen-sulfite-lyase (phosphoenolpyruvate-forming)
Requires Mg2+. The enzyme from Methanococcus jannaschii catalyses the Michael addition of sulfite to phosphoenolpyruvate. It specifically requires phosphoenolpyruvate and its broad alkaline pH optimum suggests that it uses sulfite rather than bisulfite.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
(2R)-O-phospho-3-sulfolactate sulfo-lyase
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(2R)-phospho-3-sulfolactate synthase
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ComA
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phosphosulfolactate synthase
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CAS REGISTRY NUMBER
COMMENTARY
473575-53-0
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
sulfite + phosphoenolpyruvate
(2R)-O-phospho-3-sulfolactate
show the reaction diagram
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sulfite + phosphoenolpyruvate
(2R)-O-phospho-3-sulfolactate
show the reaction diagram
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enantiomeric ratio of 84:16 R-isomer:S-isomer
ir
bisulfite + phosphoenolpyruvate
(2R)-O-phospho-3-sulfolactate
show the reaction diagram
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enzyme is involved in the biosynthesis of sulfoquinovosyl diacylglycerol, one of the major sulfur-containing glycolipids in the chloroplast thylakoid membrane
r
additional information
?
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catalyzes the first step in coenzyme M biosynthesis
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
sulfite + phosphoenolpyruvate
(2R)-O-phospho-3-sulfolactate
show the reaction diagram
Q57703
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additional information
?
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Q57703
catalyzes the first step in coenzyme M biosynthesis
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
phosphoenolpyruvate
substrate inhibition above 5 mM
sulfite
substrate inhibition above 3 mM
MgCl2
inhibition above 10 mM
additional information
further weak inhibitors, not inhibitory: K+, Na+, NH4+
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
9.8
phosphoenolpyruvate
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.9
phosphoenolpyruvate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Bacillus subtilis (strain 168)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
86700
gel filtration
639025
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
trimer
3 * 32000, SDS-PAGE, 3 * 28000, deduced from gene sequence
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 20 mM Tris-HCl, 1 mM MgCl2, pH 8.0, 2 months, no loss of activity
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
K137N
no measurable enzymatic activity
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
5 mM MgCl2 restores activity of EDTA-treated enzyme
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
agriculture
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selenium contamination is one of the most serious problems in agriculture in Se-rich regions, cpSL transgenic plants can be used for Se phytoremediation under field conditions, Selenium accumulation is significantly enhanced in the shoots of the cpSL transgenic plants compared to wild type, cpSL plants are more tolerant to the toxic sediment-soil than the wild type