Information on EC 4.3.2.6 - gamma-L-glutamyl-butirosin B gamma-glutamyl cyclotransferase

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The expected taxonomic range for this enzyme is: Bacillus circulans

EC NUMBER
COMMENTARY hide
4.3.2.6
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RECOMMENDED NAME
GeneOntology No.
gamma-L-glutamyl-butirosin B gamma-glutamyl cyclotransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
gamma-L-glutamyl-butirosin B = butirosin B + 5-oxoproline
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
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butirosin biosynthesis
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Neomycin, kanamycin and gentamicin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
gamma-L-glutamyl-butirosin B gamma-glutamyl cyclotransferase (5-oxoproline producing)
The enzyme catalyses the last step in the biosynthesis of the aminoglycoside antibiotic butirosin B. The enzyme acts as a cyclotransferase, cleaving the amide bond via transamidation using the alpha-amine of the terminal gamma-L-glutamate of the side chain, releasing it as the cyclic 5-oxoproline.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
gamma-L-glutamyl-butirosin B
butirosin B + 5-oxoproline
show the reaction diagram
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18100
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1 * 18100, calculated from amino acid sequence
20093
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1 * 20093, His-tagged enzyme, LC-ESI mass spectrometry
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni2+-NTA affinity column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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