Information on EC 4.3.2.6 - gamma-L-glutamyl-butirosin B gamma-glutamyl cyclotransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Bacillus circulans

EC NUMBER
COMMENTARY
4.3.2.6
-
RECOMMENDED NAME
GeneOntology No.
gamma-L-glutamyl-butirosin B gamma-glutamyl cyclotransferase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
gamma-L-glutamyl-butirosin B = butirosin B + 5-oxoproline
show the reaction diagram
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
Butirosin and neomycin biosynthesis
-
butirosin biosynthesis
-
SYSTEMATIC NAME
IUBMB Comments
gamma-L-glutamyl-butirosin B gamma-glutamyl cyclotransferase (5-oxoproline producing)
The enzyme catalyses the last step in the biosynthesis of the aminoglycoside antibiotic butirosin B. The enzyme acts as a cyclotransferase, cleaving the amide bond via transamidation using the alpha-amine of the terminal gamma-L-glutamate of the side chain, releasing it as the cyclic 5-oxoproline.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
btrG
-
-
-
-
btrG
Bacillus circulans NR3312
-
-
-
gamma-glutamyl cyclotransferase
-
-
gamma-glutamyl cyclotransferase
Bacillus circulans NR3312
-
-
-
GGC
Bacillus circulans NR3312
-
-
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Bacillus circulans NR3312
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
-
the enzyme is responsible for the deprotection of the gamma-L-glutamyl-(S)-4-amino-2-hydroxybutyrate side chain following transfer onto ribostamycin
metabolism
Bacillus circulans NR3312
-
the enzyme is responsible for the deprotection of the gamma-L-glutamyl-(S)-4-amino-2-hydroxybutyrate side chain following transfer onto ribostamycin
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
aminoglycosyl-gamma-L-glutamyl-butirosin B
aminoglycosyl-butirosin B + L-glutamate
show the reaction diagram
-
-
-
-
?
gamma-L-glutamyl-butirosin B
butirosin B + 5-oxoproline
show the reaction diagram
-
-
-
-
?
gamma-L-glutamyl-butirosin B
butirosin B + 5-oxoproline
show the reaction diagram
-
-
-
-
ir
gamma-L-glutamyl-butirosin B
butirosin B + 5-oxoproline
show the reaction diagram
Bacillus circulans NR3312
-
-
-
-
?
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
monomer
-
1 * 18100, calculated from amino acid sequence; 1 * 20093, His-tagged enzyme, LC-ESI mass spectrometry
monomer
Bacillus circulans NR3312
-
1 * 18100, calculated from amino acid sequence; 1 * 20093, His-tagged enzyme, LC-ESI mass spectrometry
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Ni2+-NTA affinity column chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
-