Information on EC 4.3.2.4 - purine imidazole-ring cyclase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
4.3.2.4
-
RECOMMENDED NAME
GeneOntology No.
purine imidazole-ring cyclase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
DNA 4,6-diamino-5-formamidopyrimidine = DNA adenine + H2O
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C-N bond formation
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
DNA-4,6-diamino-5-formamidopyrimidine C8-N9-lyase (cyclizing; DNA-adenine-forming)
Also acts on 2,6-diamino-5-formamido-3,4-dihydro-4-oxopyrimidine residues. Brings about the reclosure of the imidazole rings of purine residues damaged by gamma-rays.
CAS REGISTRY NUMBER
COMMENTARY hide
95990-28-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
vero cell line
-
-
Manually annotated by BRENDA team
V-79 cell line
-
-
Manually annotated by BRENDA team
strain B and pol A1 mutant
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-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
DNA 2,6-diamino-4-oxo-5-formamidopyrimidine
DNA guanine + H2O
show the reaction diagram
DNA 4,6-diamino-5-formamidopyrimidine
DNA adenine + H2O
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000007
2,6-diamino-4-oxo-5-formamidopyrimidine
-
imidazole-ring-opened guanine
0.000005
4,6-diamino-5-formamidopyrimidine
-
imidazole-ring-opened adenine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
African green monkey kidney cells
Manually annotated by BRENDA team
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 1 month, 10% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
from strain B and pol A1 mutant, partial
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partial