Information on EC 4.3.1.9 - glucosaminate ammonia-lyase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.3.1.9
-
RECOMMENDED NAME
GeneOntology No.
glucosaminate ammonia-lyase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-glucosaminate = 2-dehydro-3-deoxy-D-gluconate + NH3
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
-
-
of NH3, C-N bond cleavage
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
Pentose phosphate pathway
-
-
SYSTEMATIC NAME
IUBMB Comments
D-glucosaminate ammonia-lyase (isomerizing; 2-dehydro-3-deoxy-D-gluconate-forming)
Contains pyridoxal phosphate. The enzyme releases an unstable enamine product that tautomerizes to an imine form, which undergoes spontaneous hydrolytic deamination to form the final product.
CAS REGISTRY NUMBER
COMMENTARY hide
37290-91-8
-
9032-93-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Soil bacterium
unidentified
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-galactosaminate
?
show the reaction diagram
-
-
-
-
-
D-glucosaminate
2-dehydro-3-deoxy-D-gluconate
show the reaction diagram
D-glucosaminate
2-dehydro-3-deoxy-D-gluconate + NH3
show the reaction diagram
alpha,beta-elimination reaction
-
?
D-glucosaminate
pyruvate + glyceraldehyde + NH3
show the reaction diagram
aldolase reaction
-
?
D-mannosaminate
?
show the reaction diagram
-
-
-
-
-
D-serine
pyruvate + NH3
show the reaction diagram
-
-
-
-
-
D-threonine
2-oxobutanoate + NH3
show the reaction diagram
-
-
-
-
-
DL-allothreonine
3-hydroxy-2-oxobutanoate + NH3
show the reaction diagram
-
-
-
-
-
L-serine
pyruvate + NH3
show the reaction diagram
-
-
-
-
-
thioredoxin + NADP+
? + NADPH
show the reaction diagram
alpha2 homodimer also has thioredoxin reductase activity
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
Soil bacterium
-
required for activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0113 - 8.8
D-glucosaminic acid
0.0113
pyridoxal 5'-phosphate
pH 8
0.0044
thioredoxin
pH 8
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
140 - 240
Soil bacterium
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.7 - 8.1
Soil bacterium
-
phosphate buffer
8.3
-
potassium phosphate buffer
9
-
Tris/HCl and NaHCO3/Na2CO3 buffer
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33900
2 * 33900, alpha2-homodimer, SDS-PAGE
35000
2 * 35000, alpha,beta-heterodimer, SDS-PAGE
45000
2 * 45000, beta2-homodimer, SDS-PAGE
70000
alpha2-homodimer, SDS-PAGE
80000
alphabeta-heterodimer, SDS-PAGE
90000
beta2-homodimer, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 33900, alpha2-homodimer, SDS-PAGE; 2 * 35000, alpha,beta-heterodimer, SDS-PAGE; 2 * 45000, beta2-homodimer, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-18°C, 6 months, substantial decrease of activity
Soil bacterium
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE