Information on EC 4.3.1.30 - dTDP-4-amino-4,6-dideoxy-D-glucose ammonia-lyase

Word Map on EC 4.3.1.30
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Streptomyces venezuelae

EC NUMBER
COMMENTARY hide
4.3.1.30
-
RECOMMENDED NAME
GeneOntology No.
dTDP-4-amino-4,6-dideoxy-D-glucose ammonia-lyase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose + S-adenosyl-L-methionine + reduced acceptor = dTDP-3-dehydro-4,6-dideoxy-alpha-D-glucopyranose + NH3 + L-methionine + 5'-deoxyadenosine + acceptor
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
-
-
dTDP-D-desosamine biosynthesis
-
-
Polyketide sugar unit biosynthesis
-
-
dTDPLrhamnose biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose ammonia lyase (dTDP-3-dehydro-4,6-dideoxy-D-glucopyranose-forming)
The enzyme, which is a member of the 'AdoMet radical' (radical SAM) family, is involved in biosynthesis of TDP-alpha-D-desosamine. The reaction starts by the transfer of an electron from the reduced form of the enzyme's [4Fe-4S] cluster to S-adenosyl-L-methionine, spliting it into methionine and the radical 5-deoxyadenosin-5'-yl, which attacks the sugar substrate.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose + S-adenosyl-L-methionine
dTDP-3-dehydro-4,6-dideoxy-alpha-D-glucopyranose + NH3 + L-methionine + 5'-deoxyadenosine
show the reaction diagram
dTDP-4-amino-4,6-dideoxy-D-glucopyranose + S-adenosyl-L-methionine
dTDP-3-dehydro-4,6-dideoxy-alpha-D-glucopyranose + NH3 + L-methionine + 5'-deoxyadenosine
show the reaction diagram
-
-
-
-
?
TDP-D-quinovose + S-adenosyl-L-methionine
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose + S-adenosyl-L-methionine
dTDP-3-dehydro-4,6-dideoxy-alpha-D-glucopyranose + NH3 + L-methionine + 5'-deoxyadenosine
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4Fe-4S-center
S-adenosyl-L-methionine
additional information
-
in the experiment, the [4Fe-4S]2+ of DesII is reduced to [4Fe-4S]1+ by stepwise electron transfer from NADPH through flavodoxin and flavodoxin reductase. Accordingly, a system consisting of flavodoxin/flavodoxin reductase-NADPH or its equivalent from the cellular pool may serve as the in vivo reducing system for the DesII-catalyzed reaction
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
TDP-D-quinovose
-
substrate inhibition
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05
dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose
-
pH 8, 25°C, in presence of 0.1 mM S-adenosyl-L-methionine
0.027
S-adenosyl-L-methionine
-
pH 8.0, temperature not specified in the publication
0.2
TDP-D-quinovose
-
pH 8.0, temperature not specified in the publication
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.017
dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose
Streptomyces venezuelae
-
pH 8, 25°C, in presence of 0.1 mM S-adenosyl-L-methionine
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.33
dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose
Streptomyces venezuelae
-
pH 8, 25°C, in presence of 0.1 mM S-adenosyl-L-methionine
15135
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48000
-
gel filtration
54265
-
1 * 54265, His-tag-DesII, His-tag-DesII is a monomer in solution. It is not clear that the observed monomeric state is due to the presence of the His-tag, calculated from sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 54265, His-tag-DesII, His-tag-DesII is a monomer in solution. It is not clear that the observed monomeric state is due to the presence of the His-tag, calculated from sequence
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purification of C-terminal His6-tagged DesII from Escherichia coli BL21 Star (DE3)-desII/pET24 cells
-