Information on EC 4.3.1.22 - 3,4-dihydroxyphenylalanine reductive deaminase

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The expected taxonomic range for this enzyme is: Rhodobacter sphaeroides

EC NUMBER
COMMENTARY hide
4.3.1.22
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RECOMMENDED NAME
GeneOntology No.
3,4-dihydroxyphenylalanine reductive deaminase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-dopa + NADH = 3,4-dihydroxyphenylpropanoate + NAD+ + NH3
show the reaction diagram
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Tyrosine metabolism
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SYSTEMATIC NAME
IUBMB Comments
3,4-dihydroxy-L-phenylalanine ammonia-lyase (3,4-dihydroxyphenylpropanoate-forming)
Forms part of the L-phenylalanine-catabolism pathway in the anoxygenic phototrophic bacterium Rhodobacter sphaeroides OU5. NADPH is oxidized more slowly than NADH.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,4-dihydroxy-L-phenylalanine + NADH
3,4-dihydroxyphenylpropanoate + NAD+ + NH3
show the reaction diagram
3,4-dihydroxy-L-phenylalanine + NADPH
3,4-dihydroxyphenylpropanoate + NADP+ + NH3
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
-
less than 40% of the activity with NADH
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.21
3,4-dihydroxy-L-phenylalanine
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
39000
-
1 * 110000 + 1 * 82000 + 1 * 43000 + 1 * 39000, SDS-PAGE
43000
-
1 * 110000 + 1 * 82000 + 1 * 43000 + 1 * 39000, SDS-PAGE
82000
-
1 * 110000 + 1 * 82000 + 1 * 43000 + 1 * 39000, SDS-PAGE
110000
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1 * 110000 + 1 * 82000 + 1 * 43000 + 1 * 39000, SDS-PAGE
274000
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non-denaturing PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE