Information on EC 4.3.1.22 - 3,4-dihydroxyphenylalanine reductive deaminase

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The expected taxonomic range for this enzyme is: Rhodobacter sphaeroides

EC NUMBER
COMMENTARY
4.3.1.22
-
RECOMMENDED NAME
GeneOntology No.
3,4-dihydroxyphenylalanine reductive deaminase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
L-dopa + 2 NADH = 3,4-dihydroxyphenylpropanoate + 2 NAD+ + NH3
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Tyrosine metabolism
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-
SYSTEMATIC NAME
IUBMB Comments
3,4-dihydroxy-L-phenylalanine ammonia-lyase (3,4-dihydroxyphenylpropanoate-forming)
Forms part of the L-phenylalanine-catabolism pathway in the anoxygenic phototrophic bacterium Rhodobacter sphaeroides OU5. NADPH is oxidized more slowly than NADH.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
DOPA-reductive deaminase
-
-
DOPA-reductive deaminase
Rhodobacter sphaeroides OU5
-
-
-
DOPARDA
Rhodobacter sphaeroides OU5
-
-
-
reductive deaminase
-
-
reductive deaminase
Rhodobacter sphaeroides OU5
-
-
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Rhodobacter sphaeroides OU5
strain OU5
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,4-dihydroxy-L-phenylalanine + NADH
3,4-dihydroxyphenylpropanoate + NAD+ + NH3
show the reaction diagram
Rhodobacter sphaeroides, Rhodobacter sphaeroides OU5
-
-
-
-
?
3,4-dihydroxy-L-phenylalanine + NADPH
3,4-dihydroxyphenylpropanoate + NADP+ + NH3
show the reaction diagram
Rhodobacter sphaeroides, Rhodobacter sphaeroides OU5
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activity with NADPH is less than 40% of the activity with NADH
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NADPH
-
less than 40% of the activity with NADH
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.21
3,4-dihydroxy-L-phenylalanine
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
274000
-
non-denaturing PAGE
677089
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
tetramer
-
1 * 110000 + 1 * 82000 + 1 * 43000 + 1 * 39000, SDS-PAGE
tetramer
Rhodobacter sphaeroides OU5
-
1 * 110000 + 1 * 82000 + 1 * 43000 + 1 * 39000, SDS-PAGE
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE