Information on EC 4.3.1.22 - 3,4-dihydroxyphenylalanine reductive deaminase

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The expected taxonomic range for this enzyme is: Rhodobacter sphaeroides

EC NUMBER
COMMENTARY
4.3.1.22
-
RECOMMENDED NAME
GeneOntology No.
3,4-dihydroxyphenylalanine reductive deaminase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
L-dopa + 2 NADH = 3,4-dihydroxyphenylpropanoate + 2 NAD+ + NH3
show the reaction diagram
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Tyrosine metabolism
-
SYSTEMATIC NAME
IUBMB Comments
3,4-dihydroxy-L-phenylalanine ammonia-lyase (3,4-dihydroxyphenylpropanoate-forming)
Forms part of the L-phenylalanine-catabolism pathway in the anoxygenic phototrophic bacterium Rhodobacter sphaeroides OU5. NADPH is oxidized more slowly than NADH.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
DOPA-reductive deaminase
-
-
DOPA-reductive deaminase
Rhodobacter sphaeroides OU5
-
-
-
DOPARDA
Rhodobacter sphaeroides OU5
-
-
-
reductive deaminase
-
-
reductive deaminase
Rhodobacter sphaeroides OU5
-
-
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Rhodobacter sphaeroides OU5
strain OU5
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,4-dihydroxy-L-phenylalanine + NADH
3,4-dihydroxyphenylpropanoate + NAD+ + NH3
show the reaction diagram
Rhodobacter sphaeroides, Rhodobacter sphaeroides OU5
-
-
-
-
?
3,4-dihydroxy-L-phenylalanine + NADPH
3,4-dihydroxyphenylpropanoate + NADP+ + NH3
show the reaction diagram
Rhodobacter sphaeroides, Rhodobacter sphaeroides OU5
-
activity with NADPH is less than 40% of the activity with NADH
-
-
?
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
NADPH
-
less than 40% of the activity with NADH
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.21
-
3,4-dihydroxy-L-phenylalanine
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
274000
-
-
non-denaturing PAGE
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
tetramer
-
1 * 110000 + 1 * 82000 + 1 * 43000 + 1 * 39000, SDS-PAGE
tetramer
Rhodobacter sphaeroides OU5
-
1 * 110000 + 1 * 82000 + 1 * 43000 + 1 * 39000, SDS-PAGE
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE