Information on EC 4.3.1.14 - 3-Aminobutyryl-CoA ammonia-lyase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.3.1.14
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RECOMMENDED NAME
GeneOntology No.
3-Aminobutyryl-CoA ammonia-lyase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-3-aminobutyryl-CoA = crotonoyl-CoA + NH3
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Deamination
Double-bond formation
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-lysine fermentation to acetate and butanoate
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Lysine degradation
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SYSTEMATIC NAME
IUBMB Comments
L-3-aminobutyryl-CoA ammonia-lyase (crotonoyl-CoA-forming)
Hydroxylamine can replace ammonia as a substrate. Crotonoyl-pantetheine can replace crotonoyl-CoA but it is a poorer substrate.
CAS REGISTRY NUMBER
COMMENTARY hide
55467-41-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Anaerobic bacteria
environmental sample obtained from anaerobic digesters, putative lysine-fermenting bacteria like Fusobacterium nucleatum subsp. nucleatum, Fusobacterium nucleatum subsp. vincentii, Thermoanaerobacter tengcongensis, Porphyromonas gingivalis, Alkaliphilus metalliredigenes, Myxococcus xanthus, Anaeromyxobacter dehlogenans, Clostridium sp., Rhodoferax ferrireducens, Symbiobacterium thermophilum, Nocarioides, Janibacter sp.
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SB4
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Manually annotated by BRENDA team
SB4
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Manually annotated by BRENDA team
ATCC 25586
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Crotonyl pantetheine + NH3
Aminobutanoylpantetheine
show the reaction diagram
Crotonyl-CoA + hydroxylamine
CoA + 3-methyl-5-isoxazolidinone
show the reaction diagram
L-3-Aminobutanoyl-CoA
Crotonyl-CoA + NH3
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6,6'-Dithiodinicotinic acid
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CaCl2
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2 mM, 28% inhibition
iodoacetamide
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crotonyl-CoA protects
KCl
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400 mM, 32% inhibition
MgCl2
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2 mM, 22% inhibition
MgSO4
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2 mM, 22% inhibition
NaCl
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400 mM, 34% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.03
3-aminobutanoyl-CoA
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0.045 - 55
crotonyl-CoA
0.07
NH4Cl
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with crotonyl-CoA as cosubstrate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9.2
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amination
9.7
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deamination
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17000
Anaerobic bacteria
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SDS-PAGE
64000
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gel filtration
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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pH 6.8, half-life: 7.5 h, 0.05 mg of protein per ml
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 3 mg/ml enzyme concentration, 4% loss of activity in 3 months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
of the recombinant proteins by HiPrep 26/10 desalting column chromatography
Anaerobic bacteria
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
in Escherichia coli
Anaerobic bacteria
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
Anaerobic bacteria
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lysine fermentation pathway