Information on EC 4.3.1.10 - serine-sulfate ammonia-lyase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
4.3.1.10
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RECOMMENDED NAME
GeneOntology No.
serine-sulfate ammonia-lyase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-serine O-sulfate + H2O = pyruvate + NH3 + sulfate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
-
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of NH3, C-N bond cleavage
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SYSTEMATIC NAME
IUBMB Comments
L-serine-O-sulfate ammonia-lyase (pyruvate-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
9054-70-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
FR
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Manually annotated by BRENDA team
FR
-
-
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-chloro-L-alanine methyl ester
?
show the reaction diagram
3-cyano-L-alanine
?
show the reaction diagram
-
-
-
-
-
beta-chloroalanine
?
show the reaction diagram
beta-ureido-alpha-aminopropionic acid
?
show the reaction diagram
-
-
-
-
-
D-cystathionine
?
show the reaction diagram
-
-
-
-
-
D-cysteine
?
show the reaction diagram
-
-
-
-
-
D-serine
?
show the reaction diagram
-
-
-
-
-
D-serine-O-sulfate
pyruvate + NH3 + SO42-
show the reaction diagram
DL-cysteine
?
show the reaction diagram
-
-
-
-
-
DL-lanthionine
?
show the reaction diagram
-
-
-
-
-
L-cystathionine + H2O
?
show the reaction diagram
-
-
-
-
-
L-cysteine
?
show the reaction diagram
-
-
-
-
-
L-cystine
?
show the reaction diagram
-
-
-
-
-
L-serine
?
show the reaction diagram
-
-
-
-
-
L-serine O-sulfate
?
show the reaction diagram
L-serine O-sulfate
pyruvate + NH3 + sulfate
show the reaction diagram
L-seryl-glycine O-sulfate
?
show the reaction diagram
L-seryl-L-alanine O-sulfate
?
show the reaction diagram
L-seryl-L-leucine O-sulfate
?
show the reaction diagram
L-seryl-L-phenylalanine O-sulfate
?
show the reaction diagram
L-seryl-L-valine O-sulfate
?
show the reaction diagram
L-threonine O-sulfate
2-oxobutanoate + NH3 + SO42-
show the reaction diagram
O-acetyl-L-serine
?
show the reaction diagram
-
-
-
-
-
O-benzyl DL-serine
?
show the reaction diagram
-
-
-
-
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O-benzyl-L-serine
?
show the reaction diagram
-
-
-
-
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O-diazoacetyl-L-serine
?
show the reaction diagram
-
-
-
-
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O-methyl DL-serine
?
show the reaction diagram
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-
-
-
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O-succinyl-L-serine
?
show the reaction diagram
-
-
-
-
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S-benzyl-L-cysteine
?
show the reaction diagram
-
-
-
-
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S-carbamoyl-L-cysteine
?
show the reaction diagram
-
-
-
-
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S-ethyl-L-cysteine
?
show the reaction diagram
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-
-
-
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S-methyl L-cysteine
?
show the reaction diagram
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S-methyl-L-cysteine
?
show the reaction diagram
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growth pattern is similar to that observed with L-serine O-sulfate
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S-sulfo-L-cysteine
?
show the reaction diagram
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-
-
-
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additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-serine O-sulfate
?
show the reaction diagram
S-methyl-L-cysteine
?
show the reaction diagram
-
growth pattern is similar to that observed with L-serine O-sulfate
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-
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additional information
?
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-Cyano-L-alanine
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10 mM, 36% inhibition and 20 mM, 50% inhibition
4-iodoacetamidosalicylic acid
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irreversible
5-aminoisophthallic acid
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50 mM, 19% inhibition
5-bromo-2-furoic acid
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10 mM, 50% inhibition
aminomalonic acid
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10 mM, 44% inhibition
Benzoic acid
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34 mM, 63% inhibition
cyanide
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1.25 mM, complete inhibition
D-glycerate sulfate
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50 mM, 25% inhibition
D-phospho-L-serine
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weak competitive
D-serine O-sulfate
DL-homocysteic acid
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50 mM, 21% inhibition; 50 mM, 23% inhibition
DL-homoserine O-sulfate
hydrazine
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25 mM, complete inhibition
hydrazine hydrochloride
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1 mM: 15% inhibition
hydroxybutyric acid sulfonate
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50 mM, 23% inhibition
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hydroxylamine
Hydroxylamine hydrochloride
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0.1 mM: 8% inhibition, 1 mM: 13% inhibiotion
iodoacetic acid
Isophthalic acid
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10 mM, 42% inhibition
L-aspartic acid
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40 mM, 20% inhibition
L-cysteic acid
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50 mM, 25% inhibition
L-cysteine S-sulfate
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50 mM, 30% inhibition
L-Glutamic acid
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40 mM, 23% inhibition
L-glycerate sulfate
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40 mM, 42% inhibition
lactic acid sulfonate
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50 mM, 25% inhibition
m-chlorobenzoate
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12.5 mM, 85% inhibition
m-Hydroxybenzoate
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20 mM, 47% inhibition
m-Iodobenzoate
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6.0 mM, 90% inhibition
m-toluic acid
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25 mM, 59% inhibition
O-acetyl-L-serine
O-phospho-L-serine
p-chloromercuribenzoate
PO43-
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non-competitive
pyridoxal 5'-phosphate
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0.06 mg/l, 25-30% inhibition
Rose bengal
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non-competitive
succinic acid
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40 mM, 42% inhibition
terephthalic acid
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50 mM, 53% inhibition
trimesic acid
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50 mM, 19% inhibition
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
50
3-chloro-L-alanine methyl ester
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2.2
D-cystathionine
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enzyme B
0.17
D-Cystine
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enzyme B
8.3
D-serine
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enzyme B
0.14
D-serine O-sulfate
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enzyme B
8.5 - 9.1
L-cystathionine
2
L-cystine
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enzyme A
2.3 - 26
L-serine O-sulfate
20
O-acetyl-L-serine
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enzyme A
2
O-benzyl-DL-serine
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enzyme B, calculated on the basis of the non-reactivity of the L-isomer
1.3
O-benzyl-L-serine
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enzyme A
1.1
S-Benzyl-L-cysteine
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enzyme A
38
S-ethyl-L-cysteine
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enzyme A
17.8 - 56
S-methyl-L-cysteine
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5 - 10
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about 65% of the maximal activity at pH 8.5 and about 35% of maximal activity at pH 10.0 in Tris-HCl buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38
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enzyme assay at
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50000
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gel filtration, beta enzyme
52000
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gel filtration, alpha enzyme
53800
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high-speed membrane osmometry, alpha enzyme
54500
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high-speed membrane osmometry, beta enzyme
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 8
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stable between
6034
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
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enzyme B, after 15 min, 30% of the starting activity towards L-serine O-sulfate, 32% towards O-benzyl-DL-serine and 38% towards L-cystathionine. Enzyme A, after 15 min, 71% of the starting activity towards L-serine O-sulfate, 75% towards O-benzyl-DL-serine and 71% towards L-cystathionine
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
complete loss of activity if enzyme-containing solution is frozen and thawed
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OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
photooxidation in the presence of Rose Bengal leads to a rapid inactivation
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6033
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stable for several weeks
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0°C, for 25 h, remaining activity of 84%
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4°C, 10% loss of activity after 1 week
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 isoenzymes
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enzyme A
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