Information on EC 4.2.99.23 - tuliposide B-converting enzyme

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The expected taxonomic range for this enzyme is: Tulipa gesneriana

EC NUMBER
COMMENTARY hide
4.2.99.23
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RECOMMENDED NAME
GeneOntology No.
tuliposide B-converting enzyme
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
6-tuliposide B = tulipalin B + D-glucose
show the reaction diagram
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-
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SYSTEMATIC NAME
IUBMB Comments
6-tuliposide B D-glucose-lyase (tulipalin B-forming)
The enzyme, characterized from pollen of the plant Tulipa gesneriana (tulip), catalyses the intramolecular transesterification of 6-tuliposide B to form the antibiotic aglycon tulipalin B as a sole product. It does not catalyse the hydrolysis of 6-tuliposide B to form a hydroxy acid. The enzyme has marginal activity with 6-tuliposide A. cf. EC 4.2.99.22, tuliposide A-converting enzyme.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cultivar Murasakizuisho
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
6-tuliposide A
tulipalin A + D-glucose
show the reaction diagram
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the activities of isoforms 1, 2 and 3 towards 6-tuliposide A are 0.41, 1.8, and 1.8% of those towards P6-tuliposide B, respectively
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-
?
6-tuliposide B
tulipalin B + D-glucose
show the reaction diagram
-
preferred substrate
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-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
6-tuliposide B
tulipalin B + D-glucose
show the reaction diagram
-
preferred substrate
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-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
phenylmethylsulfonyl fluoride
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2 mM inhibits the activity of isoforms 1, 2 and 3 by 89, 99.7 and 90.2%, respectively
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.3 - 3
6-tuliposide A
7.4 - 9.5
6-tuliposide B
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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the enzyme is specifically expressed in the pollen grains, but not in the anther cells
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47000
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2 * 47000, isoform 1, 2, and 3, SDS-PAGE
92000
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isoform 2, gel filtration
93000
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isoform 3, gel filtration
99000
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isoform 1, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
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2 * 47000, isoform 1, 2, and 3, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 10
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isoforms 1, 2 and 3 are stable in the pH range of 4.5-10.0
737001
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45 - 50
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the stable temperatures of the enzymes are up to 45°C for isoforms 1 and 2, and 50°C for isoform 3
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
butyl-Toyopearl column chromatography, DEAE-Toyopearl column chromatography, Resource Q column chromatography, and Superdex 75 gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Rosetta 2 (DE3) cells
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