Information on EC 4.2.99.22 - tuliposide A-converting enzyme

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The expected taxonomic range for this enzyme is: Tulipa gesneriana

EC NUMBER
COMMENTARY hide
4.2.99.22
-
RECOMMENDED NAME
GeneOntology No.
tuliposide A-converting enzyme
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
6-tuliposide A = tulipalin A + D-glucose
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
6-tuliposide A D-glucose-lyase (tulipalin A forming)
Isolated from the plant Tulipa gesneriana (tulip). The reaction is an intramolecular transesterification producing the lactone. The enzyme also has a weak activity with 6-tuliposide B and 6-O-benzoyl-D-glucose.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl acetate
?
show the reaction diagram
-
0.4% of the activity with 6-tuliposide A
-
-
?
6-benzoyl-D-glucose
?
show the reaction diagram
-
1.2% of the activity with 6-tuliposide A
-
-
?
6-tuliposide A
tulipalin A + D-glucose
show the reaction diagram
6-tuliposide B
tulipalin B + D-glucose
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag+
-
2 mM, 98.8% inhibition
Hg2+
-
0.2 mM, 99% inhibition
phenylmethylsulfonyl fluoride
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
12 - 18
6-tuliposide A
71 - 96
6-tuliposide B
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1400 - 2800
6-tuliposide A
510 - 610
6-tuliposide B
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7.2 - 160
6-tuliposide A
15301
6.4
6-tuliposide B
Tulipa gesneriana
R4X244
isoform TCEA-b1, pH not specified in the publication, temperature not specified in the publication
19753
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1210
isoform TCEA-b1, pH not specified in the publication, temperature not specified in the publication
2260
pH 6.5, 22°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
gene TCEA1 is transcribed in all tissues except for bulbs
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
39000
2 * 39000, SDS-PAGE
70200
-
gel filtration
85400
gel filtration
87000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10
when maintained at room temperature
723472
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
stable up to, for 30 min
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
TCEA-b genes b1-b7 are higly homologous to each other, with six to seven single-nucleotide polymorphisms
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D327N
0.1% of wild-type activity
G152A
0.1% of wild-type activity
G153A
0.01% of wild-type activity
G154A
88% of wild-type activity
H359A
0.001% of wild-type activity
H362A
6% of wild-type activity
S235A
0.01% of wild-type activity
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
-
facile method of enzyme-mediated conversion of 6-tuliposide to alpha-methylene-gamma-butyrolactone, i.e.tulipalin by use of a tuliposide-converting enzyme for the conversion of 6-tuliposides. 6-Tuliposides are extracted from tulip tissues into the corresponding tulipalins in high yields within 2 h at pH 7.0. The resulting tulipalins are selectively extracted by using several organic solvents