Information on EC 4.2.3.B40 - (E)-beta-ocimene synthase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.3.B40
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
(E)-beta-ocimene synthase
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SYSTEMATIC NAME
IUBMB Comments
geranyl diphosphate diphosphate lyase ((E)-beta-ocimene forming)
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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differences in the relative emissions of (E)-beta-ocimene and (E,E)-alpha-farnesene from accession Wassilewskija, a high-(E)-beta-ocimene emitter, and accession Columbia, a trace-(E)-beta-ocimene emitter, are attributed to allelic variation of closely related, tandem-duplicated terpene synthase genes, TPS02 and TPS03. The Wassilewskija genome contains a functional allele of TPS02 but not of TPS03, while the opposite is the case for Columbia. Recombinant proteins of the functional Wassilewskija TPS02 and Columbia TPS03 genes both show (E)-beta-ocimene and (E,E)-alpha-farnesene synthase activities. Differential subcellular compartmentalization of the two enzymes in plastids and the cytosol is responsible for the ecotype-specific differences in (E)-beta-ocimene/(E,E)-alpha-farnesene emission
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.82
(2E,6E)-farnesyl diphosphate
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accession Columbia, 30°C, pH not specified in the publication
5.12
geranyl diphosphate
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accession Columbia, 30°C, pH not specified in the publication
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression is induced in leaves by elicitor and insect treatment
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induced in plants infested with two-spotted spider mites (Tetranychus urticae), coinciding with increasing emissions of (E)-beta-ocimene as well as other volatiles, (Z)-3-hexenyl acetate and (E)-4,8-dimethyl-1,3,7-nonatriene, by the infested plants. Mechanical wounding of the leaves or application of alamethicin also induces transiently increased levels of EbOS transcripts in Lotus japonicus. Wounding or alamethicin does not result in elevated release of (E)-beta-ocimene
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mRNA is detected first in mature flower buds, and its level increases until it peaks on day 4 after anthesis. Over the next 3 days, mRNA levels decline sharply by 40% and decrease slowly thereafter
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transcripts accumulate in leaves in response to exogenous jasmonic acid treatments, lepidopteran herbivory, and lepidopteran oral secretions
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treatment with 1-aminocyclopropane-1-carboxylic acid does not cause an increase in transcripts
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