Information on EC 4.2.3.B40 - (E)-beta-ocimene synthase

Word Map on EC 4.2.3.B40
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.3.B40
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
(E)-beta-ocimene synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
geranyl diphosphate = (E)-beta-ocimene + diphosphate
show the reaction diagram
SYSTEMATIC NAME
IUBMB Comments
geranyl diphosphate diphosphate lyase ((E)-beta-ocimene forming)
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
deduced protein does not contain the RRx8W motif; member of a subfamily of the terpene synthase family designated the Tps-g group, which lacks the RRx8W motif
UniProt
Manually annotated by BRENDA team
deduced protein contains a modification of the conserved RRx8W motif of plant monoterpene synthases. The N-terminal sequence indicates the presence of a plastid transit peptide
UniProt
Manually annotated by BRENDA team
member of the Tps-b subfamily containing a RRx8W motif
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
differences in the relative emissions of (E)-beta-ocimene and (E,E)-alpha-farnesene from accession Wassilewskija, a high-(E)-beta-ocimene emitter, and accession Columbia, a trace-(E)-beta-ocimene emitter, are attributed to allelic variation of closely related, tandem-duplicated terpene synthase genes, TPS02 and TPS03. The Wassilewskija genome contains a functional allele of TPS02 but not of TPS03, while the opposite is the case for Columbia. Recombinant proteins of the functional Wassilewskija TPS02 and Columbia TPS03 genes both show (E)-beta-ocimene and (E,E)-alpha-farnesene synthase activities. Differential subcellular compartmentalization of the two enzymes in plastids and the cytosol is responsible for the ecotype-specific differences in (E)-beta-ocimene/(E,E)-alpha-farnesene emission
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
(E)-beta-ocimene + diphosphate
show the reaction diagram
-
-
-
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
show the reaction diagram
additional information
?
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0026
(2E,6E)-farnesyl diphosphate
accession Columbia, 30°C, pH not specified in the publication
0.0067
geranyl diphosphate
accession Columbia, 30°C, pH not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0048
(2E,6E)-farnesyl diphosphate
Arabidopsis thaliana
A4FVP2
accession Columbia, 30°C, pH not specified in the publication
0.034
geranyl diphosphate
Arabidopsis thaliana
A4FVP2
accession Columbia, 30°C, pH not specified in the publication
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.82
(2E,6E)-farnesyl diphosphate
Arabidopsis thaliana
A4FVP2
accession Columbia, 30°C, pH not specified in the publication
81
5.12
geranyl diphosphate
Arabidopsis thaliana
A4FVP2
accession Columbia, 30°C, pH not specified in the publication
175
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.3
calculated
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
low constitutive levels of transcripts
Manually annotated by BRENDA team
additional information
no constitutive expression in flowers, stems or roots
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
N-terminal sequence indicates the presence of a plastid transit peptide
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
66724
x * 66724, calculated
68800
x * 68800, calculated
69278
x * 69278, calculated
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression is induced in leaves by elicitor and insect treatment
induced in plants infested with two-spotted spider mites (Tetranychus urticae), coinciding with increasing emissions of (E)-beta-ocimene as well as other volatiles, (Z)-3-hexenyl acetate and (E)-4,8-dimethyl-1,3,7-nonatriene, by the infested plants. Mechanical wounding of the leaves or application of alamethicin also induces transiently increased levels of EbOS transcripts in Lotus japonicus. Wounding or alamethicin does not result in elevated release of (E)-beta-ocimene
mRNA is detected first in mature flower buds, and its level increases until it peaks on day 4 after anthesis. Over the next 3 days, mRNA levels decline sharply by 40% and decrease slowly thereafter
transcripts accumulate in leaves in response to exogenous jasmonic acid treatments, lepidopteran herbivory, and lepidopteran oral secretions
treatment with 1-aminocyclopropane-1-carboxylic acid does not cause an increase in transcripts