Information on EC 4.2.3.B19 - (-)-beta-curcumene synthase

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The expected taxonomic range for this enzyme is: Nicotiana tabacum

EC NUMBER
COMMENTARY hide
4.2.3.B19
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
(-)-beta-curcumene synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2Z,6E)-farnesyl diphosphate = (-)-beta-curcumene + diphosphate
show the reaction diagram
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-
-
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SYSTEMATIC NAME
IUBMB Comments
(2Z,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, (-)-beta-curcumene-forming)
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2Z,6E)-farnesyl diphosphate
(-)-beta-curcumene + diphosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(2Z,6E)-farnesyl diphosphate
(-)-beta-curcumene + diphosphate
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00797 - 0.01403
(2Z,6E)-farnesyl diphosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.077 - 0.095
(2Z,6E)-farnesyl diphosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6.77 - 9.66
(2Z,6E)-farnesyl diphosphate
1810
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
-
assay at
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop method, crystallographic analyses of wild-type enzyme and a promiscuous mutant, with unreactive 2-fluoro analogues ((2E,6E)-2-fluorofarnesyl diphosphate and (2Z,6E)-2-fluorofarnesyl diphosphate) of (2E,6E)- and (2Z,6E)-farnesyl diphosphate reveals catalytically relevant binding modes and distinct farnesyl chain topologies that are consistent with preorganization by the active-site for cisoid or transoid cyclization
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