Information on EC 4.2.3.99 - labdatriene synthase

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The expected taxonomic range for this enzyme is: Oryza sativa

EC NUMBER
COMMENTARY hide
4.2.3.99
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RECOMMENDED NAME
GeneOntology No.
labdatriene synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
9alpha-copalyl diphosphate = (12E)-9alpha-labda-8(17),12,14-triene + diphosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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Diterpenoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
9alpha-copalyl-diphosphate diphosphate-lyase [(12E)-9alpha-labda-8(17),12,14-triene-forming]
The enzyme from rice (Oryza sativa), expressed in Escherichia coli, also produces ent-sandaracopimara-8(14),15-diene from ent-copalyl diphosphate, another naturally occuring copalyl isomer in rice (cf. ent-sandaracopimaradiene synthase, EC 4.2.3.29).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
syn-copalyl diphosphate
syn-labda-8(17),12E,14-triene + diphosphate
show the reaction diagram
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i.e. (4aS,5R,8aS)-1,1,4a-trimethyl-6-methylidene-5-[(2E)-3-methylpenta-2,4-dien-1-yl]decahydronaphthalene
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?
additional information
?
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additionally converts ent-copalyl diphosphate to ent-pimara-8(14),15-diene, reaction of EC 4.2.3.29
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.005
syn-copalyl diphosphate
22°C, pH not specified in the publication
additional information
additional information
KM value for substrate ent-copalyl diphosphate is 0.0006 mM
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00083
syn-copalyl diphosphate
Oryza sativa
Q2QQJ5
22°C, pH not specified in the publication
additional information
additional information
Oryza sativa
Q2QQJ5
Kcat value for substrate ent-copalyl diphosphate is 0.00033 per s
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.17
syn-copalyl diphosphate
Oryza sativa
Q2QQJ5
22°C, pH not specified in the publication
6874
additional information
additional information
Oryza sativa
Q2QQJ5
Kcat/KM value for substrate ent-copalyl diphosphate is 0.55 per s and mM
2
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression as glutathione-S-transferase fusion protein