Information on EC 4.2.3.93 - delta-guaiene synthase

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The expected taxonomic range for this enzyme is: Aquilaria crassna

EC NUMBER
COMMENTARY hide
4.2.3.93
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RECOMMENDED NAME
GeneOntology No.
delta-guaiene synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2E,6E)-farnesyl diphosphate = delta-guaiene + diphosphate
show the reaction diagram
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-
-
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SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, delta-guaiene-forming)
Requires Mg2+. In Aquilaria crassna three clones of the enzyme gave about 80% delta-guaiene and 20% alpha-guaiene (see also EC 4.2.3.87). A fourth clone gave 54% delta-guaiene and 45% alpha-guaiene [2]. The enzyme from Pogostemon cablin gives 13% delta-guaiene as well as 37% (-)-patchoulol (see EC 4.2.3.70), 13% alpha-guaiene (see EC 4.2.3.87), and traces of at least ten other sesquiterpenoids [1].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
delta-guaiene + diphosphate
show the reaction diagram
-
-
delta-guaiene is the major product (61% yield)
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000685 - 0.00429
(2E,6E)-farnesyl diphosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000586 - 0.00499
(2E,6E)-farnesyl diphosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.137 - 1.836
(2E,6E)-farnesyl diphosphate
81
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 (DE) Codon plus-RIL cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the production of delta-guaiene is induced by treatment with 300 mM methyl jasmonate reaching its maximum after 12 h
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the production of delta-guaiene is induced by treatment with methyl jasmonate
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Y110S/V241E/P296S/H337Y
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the mutant shows decreased catalytic efficiency compared to the wild type enzyme
Y110S/W144R/V241E/P296S/H337Y
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the mutant shows decreased catalytic efficiency compared to the wild type enzyme