Information on EC 4.2.3.90 - 5-epi-alpha-selinene synthase

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The expected taxonomic range for this enzyme is: Nostoc

EC NUMBER
COMMENTARY hide
4.2.3.90
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RECOMMENDED NAME
GeneOntology No.
5-epi-alpha-selinene synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2E,6E)-farnesyl diphosphate = 5-epi-alpha-selinene + diphosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Sesquiterpenoid and triterpenoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(2Z,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, 5-epi-alpha-selinene-forming)
Requires Mg2+. The enzyme forms 5-epi-alpha-selinene possibly via germecrene A or a 1,6-hydride shift mechanism.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
8a-epi-alpha-selinene + diphosphate
show the reaction diagram
(E,E)-farnesyl diphosphate
8a-epi-alpha-selinene + diphosphate
show the reaction diagram
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?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0034
(2E,6E)-farnesyl diphosphate
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in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30C
0.0025
(E,E)-farnesyl diphosphate
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in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.044
(2E,6E)-farnesyl diphosphate
Nostoc sp.
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in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30C
0.063
(E,E)-farnesyl diphosphate
Nostoc punctiforme
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in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
13000
(2E,6E)-farnesyl diphosphate
Nostoc sp.
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in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30C
81
24000
(E,E)-farnesyl diphosphate
Nostoc punctiforme
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in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30C
1392
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Talon metal affinity column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells