Information on EC 4.2.3.86 - 7-epi-alpha-selinene synthase

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The expected taxonomic range for this enzyme is: Vitis vinifera

EC NUMBER
COMMENTARY hide
4.2.3.86
-
RECOMMENDED NAME
GeneOntology No.
7-epi-alpha-selinene synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2E,6E)-farnesyl diphosphate = 7-epi-alpha-selinene + diphosphate
show the reaction diagram
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Sesquiterpenoid and triterpenoid biosynthesis
-
-
valencene and 7-epi-alpha-selinene biosynthesis
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-
SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (7-epi-alpha-selinene-forming)
The recombinant enzyme from Vitis vinifera forms 49.5% (+)-valencene (cf. EC 4.2.3.73, valencene synthase) and 35.5% (-)-7-epi-alpha-selinene. Initial cyclization gives (+)-germacrene A in an enzyme bound form which is not released to the medium.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
7-epi-alpha-selinene + diphosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
7-epi-alpha-selinene + diphosphate
show the reaction diagram
Q6Q3H2
the recombinant enzyme from Vitis vinifera forms 49.5% (+)-valencene (cf EC 4.2.3.73, valencene synthase) and 35.5% (-)-7-epi-alpha-selinene
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-
?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8
calculated
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
transcripts become detectable in September, almost two months after flowering, then increase and reach a maximum at the final sample date in early October when the fruit is at peak maturity
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
64400
x * 64400, calculated from sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 64400, calculated from sequence
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
His-tagged VvValCS is expressed in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
transcript levels are low in open pre-anthesis flowers, flowers after anthesis, or at early onset of fruit development