Information on EC 4.2.3.85 - alpha-eudesmol synthase

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The expected taxonomic range for this enzyme is: Zingiber zerumbet

EC NUMBER
COMMENTARY
4.2.3.85
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RECOMMENDED NAME
GeneOntology No.
alpha-eudesmol synthase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(2E,6E)-farnesyl diphosphate + H2O = alpha-eudesmol + diphosphate
show the reaction diagram
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PATHWAY
KEGG Link
MetaCyc Link
eudesmol biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (alpha-eudesmol-forming)
The recombinant enzyme from ginger (Zingiber zerumbet) gives 62.6% beta-eudesmol, 16.8% 10-epi-gamma-eudesmol, 10% alpha-eudesmol, and 5.6% aristolene. cf. EC 4.2.3.68 (beta-eudesmol synthase) and EC 4.2.3.84 (10-epi-gamma-eudesmol synthase)
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
ZSS2
B1B1U4
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ZSS2
Zingiber zerumbet Smith
B1B1U4
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
var. Smith
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate + H2O
alpha-eudesmol + diphosphate
show the reaction diagram
B1B1U4
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products are 62.6% beta-eudesmol, 16.8% 10-epi-gamma-eudesmol, 10% alpha-eudesmol, and 5.6% aristolene
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?
(2E,6E)-farnesyl diphosphate + H2O
alpha-eudesmol + diphosphate
show the reaction diagram
Zingiber zerumbet Smith
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products are 62.6% beta-eudesmol, 16.8% 10-epi-gamma-eudesmol, 10% alpha-eudesmol, and 5.6% aristolene
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?
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
B1B1U4
strong expression, transcript levels increase rapidly in summer, peak in early August and decline significantly in fall and winter
Manually annotated by BRENDA team
Zingiber zerumbet Smith
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strong expression, transcript levels increase rapidly in summer, peak in early August and decline significantly in fall and winter
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Manually annotated by BRENDA team
B1B1U4
poor expression
Manually annotated by BRENDA team
Zingiber zerumbet Smith
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poor expression
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Manually annotated by BRENDA team
additional information
B1B1U4
no expression in leaf
Manually annotated by BRENDA team
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Escherichia coli
B1B1U4
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
B1B1U4
introduction of a gene cluster encoding six enzymes of the mevalonate pathway into Escherichia coli and coexpression with ZSS2. When supplemented with mevalonate, the engineered Escherichia coli produces a similar sesquiterpene profile to that produced in the in vitro enzyme assay, and the yield of beta-eudesmol reaches 100 mg/l
additional information
Zingiber zerumbet Smith
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introduction of a gene cluster encoding six enzymes of the mevalonate pathway into Escherichia coli and coexpression with ZSS2. When supplemented with mevalonate, the engineered Escherichia coli produces a similar sesquiterpene profile to that produced in the in vitro enzyme assay, and the yield of beta-eudesmol reaches 100 mg/l
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APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
synthesis
B1B1U4
introduction of a gene cluster encoding six enzymes of the mevalonate pathway into Escherichia coli and coexpression with ZSS2. When supplemented with mevalonate, the engineered Escherichia coli produces a similar sesquiterpene profile to that produced in the in vitro enzyme assay, and the yield of beta-eudesmol reaches 100 mg/l
synthesis
Zingiber zerumbet Smith
-
introduction of a gene cluster encoding six enzymes of the mevalonate pathway into Escherichia coli and coexpression with ZSS2. When supplemented with mevalonate, the engineered Escherichia coli produces a similar sesquiterpene profile to that produced in the in vitro enzyme assay, and the yield of beta-eudesmol reaches 100 mg/l
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