Information on EC 4.2.3.83 - beta-santalene synthase

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The expected taxonomic range for this enzyme is: Santalum

EC NUMBER
COMMENTARY
4.2.3.83
-
RECOMMENDED NAME
GeneOntology No.
beta-santalene synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(2E,6E)-farnesyl diphosphate = (-)-beta-santalene + diphosphate
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
santalene biosynthesis II
-
-
SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl diphosphate lyase (cyclizing, (-)-beta-santalene-forming)
The enzyme synthesizes a mixture of sesquiterpenoids from (2E,6E)-farnesyl diphosphate. As well as (-)-beta-santalene (+)-alpha-santalene and (-)-exo-alpha-bergamotene are formed with traces of (+)-epi-beta-santalene. See EC 4.2.3.82 [(+)-alpha-santalene synthase], and EC 4.2.3.81 [(-)-exo-alpha-bergamotene synthase].
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
(-)-beta-santalene + diphosphate
show the reaction diagram
E3W202
-
-
-
?
(2E,6E)-farnesyl diphosphate
(-)-beta-santalene + diphosphate
show the reaction diagram
E3W203
-
-
-
?
(2E,6E)-farnesyl diphosphate
(-)-beta-santalene + diphosphate
show the reaction diagram
E3W204
-
-
-
?
(2E,6E)-farnesyl diphosphate
(-)-beta-santalene + diphosphate
show the reaction diagram
E3W202
when incubated in the presence of 10 mM Mg2+ the enzyme converts (2E,6E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene
-
-
?
(2E,6E)-farnesyl diphosphate
(-)-beta-santalene + diphosphate
show the reaction diagram
E3W203
when incubated in the presence of 10 mM Mg2+ the enzyme converts (2E,6E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene
-
-
?
(2E,6E)-farnesyl diphosphate
(-)-beta-santalene + diphosphate
show the reaction diagram
E3W204
when incubated in the presence of 10 mM Mg2+ the enzyme converts (2E,6E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene
-
-
?
(2E,6E)-farnesyl diphosphate
alpha-santalene + diphosphate
show the reaction diagram
E3W203
-
products are alpha-santalene, beta-, and epi-beta-santalene, exo-alpha-bergamotene, as well as traces of alpha- and beta-farnesene
-
?
(2E,6E)-farnesyl diphosphate
beta-santalene + diphosphate
show the reaction diagram
E3W202
-
products are alpha-santalene, beta-, and epi-beta-santalene, exo-alpha-bergamotene, as well as traces of alpha- and beta-farnesene
-
?
(2E,6E)-farnesyl diphosphate
beta-santalene + diphosphate
show the reaction diagram
E3W203
-
products are alpha-santalene, beta-, and epi-beta-santalene, exo-alpha-bergamotene, as well as traces of alpha- and beta-farnesene
-
?
(2E,6E)-farnesyl diphosphate
beta-santalene + diphosphate
show the reaction diagram
E3W204
-
products are alpha-santalene, beta-, and epi-beta-santalene, exo-alpha-bergamotene, as well as traces of alpha- and beta-farnesene
-
?
(2Z,6Z)-farnesyl diphosphate
(-)-beta-santalene + diphosphate
show the reaction diagram
E3W202
when incubated in the presence of 10 mM Mg2+ the enzyme converts (2Z,6Z)-farnesyl diphosphate into alpha-endo-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene and beta-santalene
-
-
?
additional information
?
-
E3W202
Santalum album Ssy also accepts (2Z,6Z)-farnesyl diphosphate as substrate, producing a mixture of endo-alpha-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene, and beta-santalene. With substrate geranxyl diphosphate, products are linalool, geraniol, and terpineol along with traces of alpha-pinene and camphene. The product profile resulting from incubations of SaSSy with (E,E)-FPP resembles the authentic essential oil of Santalum album more closely than that of the (Z,Z)-FPP incubations
-
-
-
additional information
?
-
E3W203
Santalum austrocaledonicum Ssy also accepts (2Z,6Z)-farnesyl diphosphate as substrate, producing a mixture of endo-alpha-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene, and beta-santalene. With substrate geranyl diphosphate, products are linalool, geraniol, and terpineol along with traces of alpha-pinene and camphene
-
-
-
additional information
?
-
E3W204
Santalum spicatum Ssy also accepts (2Z,6Z)-farnesyl diphosphate as substrate, producing a mixture of endo-alpha-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene, and beta-santalene. With substrate geranyl diphosphate, products are linalool, geraniol, and terpineol along with traces of alpha-pinene and camphene
-
-
-
additional information
?
-
E3W202
Santalum album Ssy also accepts (2Z,6Z)-farnesyl diphosphate as substrate, producing a mixture of endo-alpha-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene, and beta-santalene. With substrate geranxyl diphosphate, products are linalool, geraniol, and terpineol along with traces of alpha-pinene and camphene
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
(-)-beta-santalene + diphosphate
show the reaction diagram
E3W202
-
-
-
?
(2E,6E)-farnesyl diphosphate
(-)-beta-santalene + diphosphate
show the reaction diagram
E3W203
-
-
-
?
(2E,6E)-farnesyl diphosphate
(-)-beta-santalene + diphosphate
show the reaction diagram
E3W204
-
-
-
?
(2E,6E)-farnesyl diphosphate
alpha-santalene + diphosphate
show the reaction diagram
E3W203
-
products are alpha-santalene, beta-, and epi-beta-santalene, exo-alpha-bergamotene, as well as traces of alpha- and beta-farnesene
-
?
(2E,6E)-farnesyl diphosphate
beta-santalene + diphosphate
show the reaction diagram
E3W202
-
products are alpha-santalene, beta-, and epi-beta-santalene, exo-alpha-bergamotene, as well as traces of alpha- and beta-farnesene
-
?
(2E,6E)-farnesyl diphosphate
beta-santalene + diphosphate
show the reaction diagram
E3W204
-
products are alpha-santalene, beta-, and epi-beta-santalene, exo-alpha-bergamotene, as well as traces of alpha- and beta-farnesene
-
?
additional information
?
-
E3W202
Santalum album Ssy also accepts (2Z,6Z)-farnesyl diphosphate as substrate, producing a mixture of endo-alpha-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene, and beta-santalene. With substrate geranxyl diphosphate, products are linalool, geraniol, and terpineol along with traces of alpha-pinene and camphene. The product profile resulting from incubations of SaSSy with (E,E)-FPP resembles the authentic essential oil of Santalum album more closely than that of the (Z,Z)-FPP incubations
-
-
-
additional information
?
-
E3W203
Santalum austrocaledonicum Ssy also accepts (2Z,6Z)-farnesyl diphosphate as substrate, producing a mixture of endo-alpha-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene, and beta-santalene. With substrate geranyl diphosphate, products are linalool, geraniol, and terpineol along with traces of alpha-pinene and camphene
-
-
-
additional information
?
-
E3W204
Santalum spicatum Ssy also accepts (2Z,6Z)-farnesyl diphosphate as substrate, producing a mixture of endo-alpha-bergamotene, alpha-santalene, (Z)-beta-farnesene, epi-beta-santalene, and beta-santalene. With substrate geranyl diphosphate, products are linalool, geraniol, and terpineol along with traces of alpha-pinene and camphene
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
E3W202
required; when incubated in the presence of 10 mM Mg2+ the enzyme converts (E,E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene
Mg2+
E3W203
required; when incubated in the presence of 10 mM Mg2+ the enzyme converts (E,E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene
Mg2+
E3W204
required; when incubated in the presence of 10 mM Mg2+ the enzyme converts (E,E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene
Mn2+
E3W202
when incubated in the presence of 10 mM Mg2+ the enzyme converts (E,E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene
Mn2+
E3W203
incubation in presence of Mn2+ yields mainly alpha-exo-bergamotene; when incubated in the presence of 10 mM Mg2+ the enzyme converts (E,E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene
Mn2+
E3W204
incubation in presence of Mn2+ yields mainly alpha-exo-bergamotene; when incubated in the presence of 10 mM Mg2+ the enzyme converts (E,E)-farnesyl diphosphate into alpha-santalene, beta-santalene and epi-beta-santalene,alpha-exo-bergamotene, as well as traces of alpha-farnesene and beta-farnesene. Incubations with Mn2+ yields mainly alpha-exo-bergamotene
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0014
(2E,6E)-farnesyl diphosphate
E3W202
30C, pH 8.0; pH 7.4, 25C
0.0014
(2E,6E)-farnesyl diphosphate
E3W203
30C, pH 8.0; pH 7.4, 25C
0.0014
(2E,6E)-farnesyl diphosphate
E3W204
30C, pH 8.0; pH 7.4, 25C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.34
(2E,6E)-farnesyl diphosphate
E3W202
30C, pH 8.0; pH 7.4, 25C
0.91
(2E,6E)-farnesyl diphosphate
E3W203
30C, pH 8.0; pH 7.4, 25C
2.6
(2E,6E)-farnesyl diphosphate
E3W204
30C, pH 8.0; pH 7.4, 25C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
242.9
(2E,6E)-farnesyl diphosphate
E3W202
pH 7.4, 25C
81
650
(2E,6E)-farnesyl diphosphate
E3W203
pH 7.4, 25C
81
1857
(2E,6E)-farnesyl diphosphate
E3W204
pH 7.4, 25C
81
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.4
E3W202
assay at
7.4
E3W203
assay at
7.4
E3W204
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25
E3W202
assay at
25
E3W203
assay at
25
E3W204
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
66000
E3W202
-
715604
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
E3W202
x * 65163, calculated from sequence
?
E3W203
x * 65268, calculated from sequence
?
E3W204
x * 65407, calculated from sequence
?
E3W202
x * 66000, SDS-PAGE of recombinant His-tagged protein
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli; heterologous expression of a His6-tagged protein in Escherichia coli
E3W202
expression in Escherichia coli; heterologous expression of a His6-tagged protein in Escherichia coli
E3W203
expression in Escherichia coli; heterologous expression of a His6-tagged protein in Escherichia coli
E3W204