Information on EC 4.2.3.76 - (+)-delta-selinene synthase

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The expected taxonomic range for this enzyme is: Abies grandis

EC NUMBER
COMMENTARY
4.2.3.76
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RECOMMENDED NAME
GeneOntology No.
(+)-delta-selinene synthase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(2E,6E)-farnesyl diphosphate = (+)-delta-selinene + diphosphate
show the reaction diagram
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-
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(2E,6E)-farnesyl diphosphate = (+)-delta-selinene + diphosphate
show the reaction diagram
enzyme utilizes both cisoid and transoid-nerolidyl diphosphate as cyclization intermediates
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PATHWAY
KEGG Link
MetaCyc Link
oleoresin sesquiterpene volatiles biosynthesis
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Sesquiterpenoid and triterpenoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase [(+)-delta-selinene-forming]
Initial cyclization gives germacrene C in an enzyme bound form which is not released to the medium.
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
(+)-delta-selinene + diphosphate
show the reaction diagram
O64404
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main products are 12.3% delta-selinene, 17.2% germacrene A, 14.2% germacrene C, 33.2% germacrene B
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?
(2E,6E)-farnesyl diphosphate
(+)-delta-selinene + diphosphate
show the reaction diagram
-
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main products are 25.3% delta-selinene, 17.4% (E,E)-germacrene B, 9.7% guaia-6,9-diene, plus 31 further products. Most of the germacrenes produced bear a trans-C2,C3-double bond, revealing the transoid nature of the nerolidyl diphosphate intermediate
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?
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
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X * 67600, calculated
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partial
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Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Escherichia coli
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ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D331A
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mutation results in main products 24.6% delta-amorphene, 12.9 beta-bisabolene, 22.0% germacrene A
D331E
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mutation results in main products 16.6% beta-bisabolene, 29.3% germacrene A, 9.3% alpha-bisabolene
D475A
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mutation results in main products 16.6% beta-bisabolene, 29.1% germacrene A, 8.8% alpha-bisabolene
D475E
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mutation results in main products 13.4% siberene, 27.4% amorphene, 9.1% beta-bisabolene, 10.9% germacrene A
D475N
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mutation results in main products 16.8% siberene, 34.7% amorphene, 8.2% beta-bisabolene, 6.8% germacrene A
D566N
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mutation results in main products 20.6% beta-bisabolene, 29.1% germacrene A, 8.4% alpha-bisabolene
H16A
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mutation results in main products 2.0% delta-selinene, 19.5% germacrene A, 33.7% germacrene C, 21.9% germacrene B
R11A
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mutation results in main products 2.5% delta-selinene, 18.2% germacrene A, 33.2% germacrene C, 26.5% germacrene B
R12A
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mutation results in main products 3.4% delta-selinene, 17.7% germacrene A, 35.8% germacrene C, 28.1% germacrene B
Y552F
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mutation results in main products 9.1% dleta-selinene, 17.0% germacrene A, 22.9% germacrene C, 33.4% germacrene B
H17A
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mutation results in main products 1.5% delta-selinene, 19.8% germacrene A, 34.0% germacrene C, 21.8% germacrene B
additional information
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truncation at residue R11 results in main products 8.7% delta-selinene, 17.7% germacrene A, 21.8% germacrene C, 32.4% germacrene B. Truncation at residue H16 results in main products 1.8% delta-selinene, 17.0% germacrene A, 27.6% germacrene C, 19.7% germacrene B, 18.0% gamma-humulene. Truncation at residue W21 results in main products 23.4% beta-bisabolene, 24.4% germacrene A, 15.2% germacrene C, 4.7% germacrene B, 26.4% gamma-humulene