Information on EC 4.2.3.74 - presilphiperfolanol synthase

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The expected taxonomic range for this enzyme is: Botrytis cinerea

EC NUMBER
COMMENTARY hide
4.2.3.74
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RECOMMENDED NAME
GeneOntology No.
presilphiperfolanol synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2E,6E)-farnesyl diphosphate + H2O = presilphiperfolan-8beta-ol + diphosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
botrydial biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphohydrolase (presilphiperfolan-8beta-ol-forming)
Requires Mg2+. Presilphiperfolan-8beta-ol is the precursor of botrydial, a phytotoxic sesquiterpene metabolite secreted by the fungus Botryotinia fuckeliana (Botrytis cinerea), the causal agent of gray mold disease in plants.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate + H2O
presilphiperfolan-8beta-ol + diphosphate
show the reaction diagram
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?
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00604
(2E,6E)-farnesyl diphosphate
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recombinant enzyme, in 50 mM PIPES, 10 mM MgCl2, 100 mM NaCl, pH 7.0, at 30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.23
(2E,6E)-farnesyl diphosphate
Botrytis cinerea
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recombinant enzyme, in 50 mM PIPES, 10 mM MgCl2, 100 mM NaCl, pH 7.0, at 30°C
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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