Information on EC 4.2.3.74 - presilphiperfolanol synthase

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The expected taxonomic range for this enzyme is: Botrytis cinerea

EC NUMBER
COMMENTARY
4.2.3.74
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RECOMMENDED NAME
GeneOntology No.
presilphiperfolanol synthase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(2E,6E)-farnesyl diphosphate + H2O = presilphiperfolan-8beta-ol + diphosphate
show the reaction diagram
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PATHWAY
KEGG Link
MetaCyc Link
botrydial biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphohydrolase (presilphiperfolan-8beta-ol-forming)
Requires Mg2+. Presilphiperfolan-8beta-ol is the precursor of botrydial, a phytotoxic sesquiterpene metabolite secreted by the fungus Botryotinia fuckeliana (Botrytis cinerea), the causal agent of gray mold disease in plants.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
BOT2
Botryotinia fuckeliana
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PSPS
Botryotinia fuckeliana
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Botryotinia fuckeliana
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-
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
Botryotinia fuckeliana
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Botrytis cinerea BOT2 deletion mutants are blocked in the first step of botrydial biosynthesis
malfunction
Botryotinia fuckeliana
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inactivation of the BOT2 gene results in strain-dependent loss of virulence
metabolism
Botryotinia fuckeliana
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BOT2 is required for the first step of botrydial biosynthesis
metabolism
Botryotinia fuckeliana
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the BOT2 gene encodes the sesquiterpene synthase that is responsible for the committed step in the biosynthesis of botrydial
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate + H2O
presilphiperfolan-8beta-ol + diphosphate
show the reaction diagram
Botryotinia fuckeliana
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-
-
-
?
additional information
?
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Botryotinia fuckeliana
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the BOT2 protein is a sesquiterpene synthase
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additional information
?
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Botryotinia fuckeliana
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the conversion of (2E,6E)-farnesyl diphosphate to presilphiperfolan-8beta-ol involves initial formation of the humulyl cation which then cyclizes to the 2-epicaryophyllenyl ion. Following ring expansion and further cyclization, the resultant tricyclic cation undergoes a 1,3-hydride shift to generate the presilphiperfolan-8-yl cation which is then quenched by capture of water to give presilphiperfolan-8beta-ol
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METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mg2+
Botryotinia fuckeliana
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the BOT2 protein has both of the two strictly conserved active-site motifs that are essential to binding of three Mg2+ cations
Mg2+
Botryotinia fuckeliana
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required
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.00604
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(2E,6E)-farnesyl diphosphate
Botryotinia fuckeliana
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recombinant enzyme, in 50 mM PIPES, 10 mM MgCl2, 100 mM NaCl, pH 7.0, at 30°C
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
3.23
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(2E,6E)-farnesyl diphosphate
Botryotinia fuckeliana
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recombinant enzyme, in 50 mM PIPES, 10 mM MgCl2, 100 mM NaCl, pH 7.0, at 30°C
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Ni-NTA column chromatography
Botryotinia fuckeliana
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Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
Botryotinia fuckeliana
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