Information on EC 4.2.3.74 - presilphiperfolanol synthase

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The expected taxonomic range for this enzyme is: Botryotinia fuckeliana

EC NUMBER
COMMENTARY
4.2.3.74
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RECOMMENDED NAME
GeneOntology No.
presilphiperfolanol synthase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(2E,6E)-farnesyl diphosphate + H2O = presilphiperfolan-8beta-ol + diphosphate
show the reaction diagram
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PATHWAY
KEGG Link
MetaCyc Link
botrydial biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphohydrolase (presilphiperfolan-8beta-ol-forming)
Requires Mg2+. Presilphiperfolan-8beta-ol is the precursor of botrydial, a phytotoxic sesquiterpene metabolite secreted by the fungus Botryotinia fuckeliana (Botrytis cinerea), the causal agent of gray mold disease in plants.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
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Botrytis cinerea BOT2 deletion mutants are blocked in the first step of botrydial biosynthesis
malfunction
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inactivation of the BOT2 gene results in strain-dependent loss of virulence
metabolism
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BOT2 is required for the first step of botrydial biosynthesis
metabolism
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the BOT2 gene encodes the sesquiterpene synthase that is responsible for the committed step in the biosynthesis of botrydial
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate + H2O
presilphiperfolan-8beta-ol + diphosphate
show the reaction diagram
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?
additional information
?
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the BOT2 protein is a sesquiterpene synthase
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additional information
?
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the conversion of (2E,6E)-farnesyl diphosphate to presilphiperfolan-8beta-ol involves initial formation of the humulyl cation which then cyclizes to the 2-epicaryophyllenyl ion. Following ring expansion and further cyclization, the resultant tricyclic cation undergoes a 1,3-hydride shift to generate the presilphiperfolan-8-yl cation which is then quenched by capture of water to give presilphiperfolan-8beta-ol
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METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mg2+
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the BOT2 protein has both of the two strictly conserved active-site motifs that are essential to binding of three Mg2+ cations
Mg2+
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required
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.00604
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(2E,6E)-farnesyl diphosphate
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recombinant enzyme, in 50 mM PIPES, 10 mM MgCl2, 100 mM NaCl, pH 7.0, at 30°C
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
3.23
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(2E,6E)-farnesyl diphosphate
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recombinant enzyme, in 50 mM PIPES, 10 mM MgCl2, 100 mM NaCl, pH 7.0, at 30°C
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Ni-NTA column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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