Information on EC 4.2.3.71 - (E,E)-germacrene B synthase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.3.71
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RECOMMENDED NAME
GeneOntology No.
(E,E)-germacrene B synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2E,6E)-farnesyl diphosphate = (E,E)-germacrene B + diphosphate
show the reaction diagram
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
germacrene biosynthesis
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Sesquiterpenoid and triterpenoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase [(E,E)-germacrene-B-forming]
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
(+)-germacrene B + diphosphate
show the reaction diagram
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products are 50.2% (+)-germacrene D, 17.1% germacrene B, plus a number of minor products
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?
(2E,6E)-farnesyl diphosphate
(E,E)-germacrene B + diphosphate
show the reaction diagram
-
-
-
?
(2E,6E)-farnesyl diphosphate
germacrene B + diphosphate
show the reaction diagram
additional information
?
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no substrate: geranyl diphosphate
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
1 mM, 33% of the activity with Mg2+
Mg2+
maximum activity at 0.1 mM
Mn2+
0.1 mM, 24% of the activity with Mg2+
Ni2+
0.5 mM, 6% of the activity with Mg2+
additional information
divalent cation absolutely required, inactive with Cu2+ or Zn2+
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9
almost completely inactive below and above
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60000
x * 63800, calculated, x * 60000, SDS-PAGE
63800
x * 63800, calculated, x * 60000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 63800, calculated, x * 60000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant protein
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D331A
mutation results in main products 24.6% delta-amorphene, 12.9 beta-bisabolene, 22.0% germacrene A
D331E
mutation results in main products 16.6% beta-bisabolene, 29.3% germacrene A, 9.3% alpha-bisabolene
D475A
mutation results in main products 16.6% beta-bisabolene, 29.1% germacrene A, 8.8% alpha-bisabolene
D475E
mutation results in main products 13.4% siberene, 27.4% amorphene, 9.1% beta-bisabolene, 10.9% germacrene A
D475N
mutation results in main products 16.8% siberene, 34.7% amorphene, 8.2% beta-bisabolene, 6.8% germacrene A
D566N
mutation results in main products 20.6% beta-bisabolene, 29.1% germacrene A, 8.4% alpha-bisabolene
H16A
mutation results in main products 2.0% delta-selinene, 19.5% germacrene A, 33.7% germacrene C, 21.9% germacrene B
H17A
mutation results in main products 1.5% delta-selinene, 19.8% germacrene A, 34.0% germacrene C, 21.8% germacrene B
R11A
mutation results in main products 2.5% delta-selinene, 18.2% germacrene A, 33.2% germacrene C, 26.5% germacrene B
R12A
mutation results in main products 3.4% delta-selinene, 17.7% germacrene A, 35.8% germacrene C, 28.1% germacrene B
Y552F
mutation results in main products 9.1% dleta-selinene, 17.0% germacrene A, 22.9% germacrene C, 33.4% germacrene B
additional information
truncation at residue R11 results in main products 8.7% delta-selinene, 17.7% germacrene A, 21.8% germacrene C, 32.4% germacrene B. Truncation at residue H16 results in main products 1.8% delta-selinene, 17.0% germacrene A, 27.6% germacrene C, 19.7% germacrene B, 18.0% gamma-humulene. Truncation at residue W21 results in main products 23.4% beta-bisabolene, 24.4% germacrene A, 15.2% germacrene C, 4.7% germacrene B, 26.4% gamma-humulene