Information on EC 4.2.3.66 - beta-selinene cyclase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY
4.2.3.66
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RECOMMENDED NAME
GeneOntology No.
beta-selinene cyclase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(2E,6E)-farnesyl diphosphate = beta-selinene + diphosphate
show the reaction diagram
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-
-
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PATHWAY
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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selinene biosynthesis
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Sesquiterpenoid and triterpenoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (beta-selinene-forming)
Initial cyclization gives (+)-germacrene A in an enzyme bound form which is not released to the medium.
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
× Citrofortunella mitis
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
beta-selinene + diphosphate
show the reaction diagram
× Citrofortunella mitis
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-
-
-
?
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mg2+
× Citrofortunella mitis
-
maximum activity at 10 mM
Mn2+
× Citrofortunella mitis
-
about 60% of the activity with Mg2+, inhibitory above 0.2 mM
additional information
× Citrofortunella mitis
-
presence of divalent metal ion required, Mg2+ is preferred
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Mn2+
× Citrofortunella mitis
-
inhibitory above 0.2 mM, stimulation below
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.045
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(2E,6E)-farnesyl diphosphate
× Citrofortunella mitis
-
pH 7.6, 28°C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5180
-
× Citrofortunella mitis
-
pH 7.6, 28°C
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
-
× Citrofortunella mitis
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
× Citrofortunella mitis
-
-
Manually annotated by BRENDA team
× Citrofortunella mitis
-
flavedo of immature fruit
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
67000
-
× Citrofortunella mitis
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gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
monomer
× Citrofortunella mitis
-
1 * 67000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partial
× Citrofortunella mitis
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