Information on EC 4.2.3.60 - germacrene C synthase

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The expected taxonomic range for this enzyme is: Abies grandis

EC NUMBER
COMMENTARY
4.2.3.60
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RECOMMENDED NAME
GeneOntology No.
germacrene C synthase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(2E,6E)-farnesyl diphosphate = germacrene C + diphosphate
show the reaction diagram
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-
-
-
PATHWAY
KEGG Link
MetaCyc Link
germacrene biosynthesis
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Sesquiterpenoid and triterpenoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (germacrene-C-forming)
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
germacrene C + diphosphate
show the reaction diagram
O64404
-
main products are 12.3% delta-selinene, 17.2% germacrene A, 14.2% germacrene C, 33.2% germacrene B
-
?
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partial
O64404
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Escherichia coli
O64404
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D331A
O64404
mutation results in main products 24.6% delta-amorphene, 12.9 beta-bisabolene, 22.0% germacrene A
D331E
O64404
mutation results in main products 16.6% beta-bisabolene, 29.3% germacrene A, 9.3% alpha-bisabolene
D475A
O64404
mutation results in main products 16.6% beta-bisabolene, 29.1% germacrene A, 8.8% alpha-bisabolene
D475E
O64404
mutation results in main products 13.4% siberene, 27.4% amorphene, 9.1% beta-bisabolene, 10.9% germacrene A
D475N
O64404
mutation results in main products 16.8% siberene, 34.7% amorphene, 8.2% beta-bisabolene, 6.8% germacrene A
D566N
O64404
mutation results in main products 20.6% beta-bisabolene, 29.1% germacrene A, 8.4% alpha-bisabolene
H16A
O64404
mutation results in main products 2.0% delta-selinene, 19.5% germacrene A, 33.7% germacrene C, 21.9% germacrene B
R11A
O64404
mutation results in main products 2.5% delta-selinene, 18.2% germacrene A, 33.2% germacrene C, 26.5% germacrene B
R12A
O64404
mutation results in main products 3.4% delta-selinene, 17.7% germacrene A, 35.8% germacrene C, 28.1% germacrene B
Y552F
O64404
mutation results in main products 9.1% dleta-selinene, 17.0% germacrene A, 22.9% germacrene C, 33.4% germacrene B
H17A
O64404
mutation results in main products 1.5% delta-selinene, 19.8% germacrene A, 34.0% germacrene C, 21.8% germacrene B
additional information
O64404
truncation at residue R11 results in main products 8.7% delta-selinene, 17.7% germacrene A, 21.8% germacrene C, 32.4% germacrene B. Truncation at residue H16 results in main products 1.8% delta-selinene, 17.0% germacrene A, 27.6% germacrene C, 19.7% germacrene B, 18.0% gamma-humulene. Truncation at residue W21 results in main products 23.4% beta-bisabolene, 24.4% germacrene A, 15.2% germacrene C, 4.7% germacrene B, 26.4% gamma-humulene