Information on EC 4.2.3.49 - (3R,6E)-nerolidol synthase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.3.49
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RECOMMENDED NAME
GeneOntology No.
(3R,6E)-nerolidol synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2E,6E)-farnesyl diphosphate + H2O = (3R,6E)-nerolidol + diphosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
(3E)-4,8-dimethylnona-1,3,7-triene biosynthesis
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Sesquiterpenoid and triterpenoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase [(3R,6E)-nerolidol-forming]
The enzyme catalyses a step in the formation of (3E)-4,8-dimethylnona-1,3,7-triene, a key signal molecule in induced plant defense mediated by the attraction of enemies of herbivores [1]. Nerolidol is a naturally occurring sesquiterpene found in the essential oils of many types of plants.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cultivar B73 and Delprim
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate + H2O
(3R,6E)-nerolidol + diphosphate
show the reaction diagram
additional information
?
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Populus trichocarpa TPS3 additionally accepts geranyl diphosphate to produce (3R)-linalool. No substrate: geranylgeranyl diphosphate
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate + H2O
(3R,6E)-nerolidol + diphosphate
show the reaction diagram
Q84ZW8
herbivore-induced production of (3R,6E)-nerolidol, an intermediate in the formation of 4,8-dimethyl-1,3(E),7-nonatriene
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
divalent metal ion cofactor is required for enzyme activity, KM-value for Mg2+: 0.47 mM. Mg2+ and Mn2+ give substantial activity, with Mg2+ at 5 mM giving the highest activity; divalent metal ion cofactor is required for enzyme activity, KM-value for Mn2+: 0.026 mM. Mg2+ and Mn2+ give substantial activity, with Mg2+ at 5 mM giving the highest activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.001
(2E,6E)-farnesyl diphosphate
30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7.7
half-maximal activity at pH 6.0 and pH 7.7
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 37
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
67400
1 * 67400, calculated from sequence; 2 * 67400, calculated from sequence
71000
monomer, gel filtration
140000
dimer, recombinant enzyme, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 67400, calculated from sequence
monomer
1 * 67400, calculated from sequence
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, stable for at least 1 week
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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overexpression in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
induced by Lymantria dispar feeding
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transcripts are detected in the leaf and sheath tissue of 2-week-old uninjured maize plants, but none is found in the roots. After 16 h of herbivory by Egyptian cotton leafworm larvae, transcript levels in the leaves of maize cv B73 are induced about 8-fold. The tps1 transcript levels in the maize cv Delprim are high even in uninjured plants and are only marginally elevated by herbivory. In older plants (3 months), moderate levels of transcript are present in leaves of both herbivore-treated and untreated plants of both cultivars