Information on EC 4.2.3.42 - aphidicolan-16beta-ol synthase

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The expected taxonomic range for this enzyme is: Phoma betae

EC NUMBER
COMMENTARY hide
4.2.3.42
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RECOMMENDED NAME
GeneOntology No.
aphidicolan-16beta-ol synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
9alpha-copalyl diphosphate + H2O = aphidicolan-16beta-ol + diphosphate
show the reaction diagram
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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Diterpenoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
9alpha-copalyl-diphosphate diphosphate-lyase (aphidicolan-16beta-ol-forming)
This is a bifunctional enzyme which also has EC 5.5.1.14 syn-copalyl diphosphate synthase activity. Aphidicolan-16beta-ol is a precursor of aphidicolin, a specific inhibitor of DNA polymerase alpha (EC 2.7.7.7).
CAS REGISTRY NUMBER
COMMENTARY hide
348150-23-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
9alpha-copalyl diphosphate + H2O
aphidicolan-16beta-ol + diphosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
9alpha-copalyl diphosphate + H2O
aphidicolan-16beta-ol + diphosphate
show the reaction diagram
Q96WT2
bifunctional enzyme which also has EC 5.5.1.14, syn-copalyl diphosphate synthase activity
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-
?
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
bifunctional enzyme which also has EC 5.5.1.14, syn-copalyl diphosphate synthase activity
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
synthesis of aphidicolin, inhibitor of DNA polymerase alpha, by heterologous expression of the genes encoding geranlygeranyl diphosphate synthase, aphidicolan-16beta-ol synthase, and monooxygenases P450-1 and P450-1 from Phoma betae in Aspergillus oryzae. Host Saccharomyces cerevisiae, carrying the genes encoding geranlygeranyl diphosphate synthase and aphidicolan-16beta-ol, produces aphidicolan-16beta-ol