Information on EC 4.2.3.41 - elisabethatriene synthase

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The expected taxonomic range for this enzyme is: Pseudopterogorgia elisabethae

EC NUMBER
COMMENTARY
4.2.3.41
-
RECOMMENDED NAME
GeneOntology No.
elisabethatriene synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
geranylgeranyl diphosphate = elisabethatriene + diphosphate
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
geranylgeranyl-diphosphate diphosphate-lyase (elisabethatriene-forming)
Requires Mg2+ or less efficiently Mn2+. The enzyme is also able to use farnesyl diphosphate and geranyl diphosphate.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
elisabethatriene cyclase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
334022-59-2
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
farnesyl diphosphate
gamma-humulene + beta-ylangene + alpha-patchoulene + diphosphate
show the reaction diagram
-
formation of 28% gamma-humulene, 43% beta-ylangene, 17% alpha-patchoulene and two minor products
-
-
?
geranyl diphosphate
? + diphosphate
show the reaction diagram
-
formation of eleven terpene reaction products
-
-
?
geranylgeranyl diphosphate
elisabethatriene + diphosphate
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
geranylgeranyl diphosphate
elisabethatriene + diphosphate
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mg2+
-
the enzyme can not catalyze the cyclization reaction in the absence of Mg2+, saturating concentration is 1.5 mM, Km-value: 0.78 mM. At a concentration of 2 mM, Mg2+ supports the cyclization reaction most efficiently. When substituting Mn2+ for Mg2+, approximately one-third of the elisabethatriene synthase activity is observed
Mn2+
-
at a concentration of 2 mM, Mg2+ supports the cyclization reaction most efficiently. When substituting Mn2+ for Mg2+, approximately one-third of the elisabethatriene synthase activity is observed
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
-
no inhibition by 0.05 mM N-ethylmaleimide
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0035
-
farnesyl diphosphate
-
pH 7.1, 29C
0.0285
-
geranyl diphosphate
-
pH 7.1, 29C
0.0023
-
geranylgeranyl diphosphate
-
pH 7.1, 29C
0.007
-
geranylgeranyl diphosphate
-
29C
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
3400
-
farnesyl diphosphate
-
pH 7.1, 29C
1300
-
geranyl diphosphate
-
pH 7.1, 29C
41000
-
geranylgeranyl diphosphate
-
pH 7.1, 29C
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
4.9
-
diethyl diphosphate
-
29C
4.5
-
phenyl glyoxal
-
29C
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.1
-
-
highest activity in phosphate buffer
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.1
-
-
isoelectric focusing, pH-range 5-8
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
47000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
monomer
-
1 * 47000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE