Information on EC 4.2.3.40 - (Z)-gamma-bisabolene synthase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.3.40
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RECOMMENDED NAME
GeneOntology No.
(Z)-gamma-bisabolene synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2E,6E)-farnesyl diphosphate = (Z)-gamma-bisabolene + diphosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Sesquiterpenoid and triterpenoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase [(Z)-gamma-bisabolene-forming]
This sesquiterpenoid enzyme is constitutively expressed in the root, hydathodes and stigma of the plant Arabidopsis thaliana. If the leaves of the plant are wounded, e.g. by cutting, the enzyme is also induced close to the wound site. The sesquiterpenoids (E)-nerolidol and alpha-bisabolol are also produced by this enzyme as minor products.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ecotype Ler, root-specific genes At4g13280 and At4g13300 encoding isozymes TPS12 and TPS13
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
(Z)-gamma-bisabolene + diphosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
(Z)-gamma-bisabolene + diphosphate
show the reaction diagram
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-
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-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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wound-inducible (Z)-gamma-bisabolene synthase, mechanical wounding of leaves induces local expression of At4g13280 and At4g13300
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.3
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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young rosette leaves, enzyme induction by mechanical wounding
Manually annotated by BRENDA team
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root-specific (Z)-gamma-bisabolene synthase, in cortex and sub-epidermal layers
Manually annotated by BRENDA team
additional information
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enzyme tissue localization study
Manually annotated by BRENDA team
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
root-specific genes At4g13280 and At4g13300 show constitutive promoter activities in the cortex and sub-epidermal layers of Arabidopsis thaliana roots, hierarchical cluster analysis of terpene synthases TPS gene expression, expression analysis, microarray profiling, overview. Expression in Escherichia coli strain BL21
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
L405V
products are 1.9% 7-epi-sesquithujene, 8.1% (E)-alpha-bergamotene, 8.9% sesquiabinene A, 23.9% (E)-beta-farnesene, 0% gamma-curcumene, 9.7% zingiberene, 12.0% beta-bisabolene, 26.6% beta-sesquiphellandrene, 8.9% (E)-gamma-bisabolene
L405V/Y373S
products are 1.2% 7-epi-sesquithujene, 23.6% (E)-alpha-bergamotene, 17.7% sesquiabinene A, 53.0% (E)-beta-farnesene, 0% gamma-curcumene, 0.6% zingiberene, 1.3% beta-bisabolene, 2.6% beta-sesquiphellandrene, 0% (E)-gamma-bisabolene
Y373S
products are 7.6% 7-epi-sesquithujene, 24.8% (E)-alpha-bergamotene, 18.2% sesquiabinene A, 38.5% (E)-beta-farnesene, 0% gamma-curcumene, 4.7% zingiberene, 2.5% beta-bisabolene, 3.7% beta-sesquiphellandrene, 0% (E)-gamma-bisabolene