Information on EC 4.2.3.36 - terpentetriene synthase

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The expected taxonomic range for this enzyme is: Kitasatospora griseola

EC NUMBER
COMMENTARY hide
4.2.3.36
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RECOMMENDED NAME
GeneOntology No.
terpentetriene synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
terpentedienyl diphosphate = terpentetriene + diphosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
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terpentecin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
terpentedienyl-diphosphate diphosphate-lyase (terpentetriene-forming)
Requires Mg2+ for maximal activity but can use Mn2+, Fe2+ or Co2+ to a lesser extent [2]. Following on from EC 5.5.1.15, terpentedienyl-diphosphate synthase, this enzyme completes the transformation of geranylgeranyl diphosphate (GGDP) into terpentetriene, which is a precursor of the diterpenoid antibiotic terpentecin. Farnesyl diphosphate can also act as a substrate.
CAS REGISTRY NUMBER
COMMENTARY hide
429681-55-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain MF730-N6
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
the CYC2 enzyme involves a DDXXD motif in the sequence that is characteristic to type-A cyclase and responsible for the diphosphate-removal reaction through Mg2+ chelation
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
terpentedienyl diphosphate
terpentetriene + diphosphate
show the reaction diagram
additional information
?
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CYC2 is not a cyclase, it shows broad substrate specificity. All of the three bicyclic diterpenes labdane, halimane, and clerodane, occuring in nature, are accepted as substrates of the CYC2, reactivity in a coupled assay with ent-CDP synthase, EC 5.5.1.13, overview
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
terpentedienyl diphosphate
terpentetriene + diphosphate
show the reaction diagram
Q9AJE3
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?
additional information
?
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Q9AJE3
CYC2 is not a cyclase, it shows broad substrate specificity. All of the three bicyclic diterpenes labdane, halimane, and clerodane, occuring in nature, are accepted as substrates of the CYC2, reactivity in a coupled assay with ent-CDP synthase, EC 5.5.1.13, overview
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
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1 mM, 3% of the activity with Mg2+, substrate: terpentedienyl diphosphate
Fe2+
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1 mM, 5% of the activity with Mg2+, substrate: terpentedienyl diphosphate
Mn2+
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1 mM, 37% of the activity with Mg2+, substrate: terpentedienyl diphosphate
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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20% glycerol, 5 mM 2-mercaptoethanol, and 0.1% Tween 80 are required for the full activity of the Cy2c2
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0076
terpentedienyl diphosphate
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
assay at; assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37000
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2 * 37000, calculated from sequence
70000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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2 * 37000, calculated from sequence
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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stable after incubation at 30°C in 0.05 M Tris-HCl buffer (pH 6.8) for 1 h
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
heterologous expression of the cyclase genes in Streptomyces lividans and Escherichia coli
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
CYC2 is a promising tool for introducing a buta-1,3-diene moiety into molecules by the enzymatic reactions