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Information on EC 4.2.3.35 - syn-pimara-7,15-diene synthase and Organism(s) Picea abies and UniProt Accession Q675L5

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.35 syn-pimara-7,15-diene synthase
IUBMB Comments
This enzyme is a class I terpene synthase . 9beta-Pimara-7,15-diene is a precursor of momilactones A and B, rice diterpenoid phytoalexins that are produced in response to attack (by a pathogen, elicitor or UV irradiation) and are involved in the defense mechanism of the plant. Momilactone B can also act as an allochemical, being constitutively produced in the root of the plant and secreted to the rhizosphere where it suppresses the growth of neighbouring plants and soil microorganisms .
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This record set is specific for:
Picea abies
UNIPROT: Q675L5
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Word Map
  • 4.2.3.35
  • phytoalexins
  • diterpene
  • sativa
  • momilactones
  • diterpenoids
  • ent-copalyl
  • cyclization
  • gibberellin
  • geranylgeranyl
  • type-a
  • phytocassanes
  • elicitor
  • cyclases
The taxonomic range for the selected organisms is: Picea abies
The enzyme appears in selected viruses and cellular organisms
Synonyms
osks4, osdts2, syn-pimara-7,15-diene synthase, 9beta-pimara-7,15-diene synthase, more
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cyclization
-
elimination of diphosphate
-
SYSTEMATIC NAME
IUBMB Comments
9alpha-copalyl-diphosphate diphosphate-lyase (9beta-pimara-7,15-diene-forming)
This enzyme is a class I terpene synthase [1]. 9beta-Pimara-7,15-diene is a precursor of momilactones A and B, rice diterpenoid phytoalexins that are produced in response to attack (by a pathogen, elicitor or UV irradiation) and are involved in the defense mechanism of the plant. Momilactone B can also act as an allochemical, being constitutively produced in the root of the plant and secreted to the rhizosphere where it suppresses the growth of neighbouring plants and soil microorganisms [1].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-copalyl-diphosphate
9beta-isopimara-7,15-diene + diphosphate
show the reaction diagram
reaction intermediate is C8-sandaracopimaradienyl cation
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
TPSD2_PICAB
867
0
99203
Swiss-Prot
-
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
homology model of the second active site of enzyme based on the structure of 5-epiaristolochene synthase from Nicotiana tabacum, Protein Data Bank ID code 5EAT
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A713S
products are isopimaradiene and sandaracopimaradiene
G651V
no change in product
V717L
no change in product
W679L/Y686H/A713S/V717L
products are isopimaradiene and sandaracopimaradiene
Y686H
no change in product
Y686H/A713S
main products are isopimaradiene and sandaracopimaradiene
Y686H/A713S A713S
main products are isopimaradiene and sandaracopimaradiene
Y686H/A713S/V717L
products are isopimaradiene and sandaracopimaradiene
additional information
swapping of residues 568–640 of isopimaradiene synhase to corresponding residues 560-632 of levopimaradiene/abietadiene synthase results in complete reversion of the product profiles of the two enzymes
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Keeling, C.I.; Weisshaar, S.; Lin, r.P.C.; Bohlmann, J.
Functional plasticity of paralogous diterpene synthases involved in conifer defense
Proc. Natl. Acad. Sci. USA
105
1085-1090
2008
Picea abies (Q675L5)
Manually annotated by BRENDA team