Information on EC 4.2.3.35 - syn-pimara-7,15-diene synthase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.3.35
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RECOMMENDED NAME
GeneOntology No.
syn-pimara-7,15-diene synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
9alpha-copalyl diphosphate = 9beta-pimara-7,15-diene + diphosphate
show the reaction diagram
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-
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cyclization
elimination of diphosphate
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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diterpene phytoalexins precursors biosynthesis
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Diterpenoid biosynthesis
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diterpene phytoalexins precursors biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
9alpha-copalyl-diphosphate diphosphate-lyase (9beta-pimara-7,15-diene-forming)
This enzyme is a class I terpene synthase [1]. 9beta-Pimara-7,15-diene is a precursor of momilactones A and B, rice diterpenoid phytoalexins that are produced in response to attack (by a pathogen, elicitor or UV irradiation) and are involved in the defense mechanism of the plant. Momilactone B can also act as an allochemical, being constitutively produced in the root of the plant and secreted to the rhizosphere where it suppresses the growth of neighbouring plants and soil microorganisms [1].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-copalyl-diphosphate
9beta-isopimara-7,15-diene + diphosphate
show the reaction diagram
reaction intermediate is C8-sandaracopimaradienyl cation
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-
?
9,10-syn-copalyl diphosphate
9beta-pimara-7,15-diene + diphosphate
show the reaction diagram
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-
product identification via radio TLC, GC and GC/MS
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?
9alpha-copalyl diphosphate
9beta-pimara-7,15-diene + diphosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
9,10-syn-copalyl diphosphate
9beta-pimara-7,15-diene + diphosphate
show the reaction diagram
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-
product identification via radio TLC, GC and GC/MS
-
?
9alpha-copalyl diphosphate
9beta-pimara-7,15-diene + diphosphate
show the reaction diagram
Q0JEZ8
the enzyme is involved in the biosynthesis of oryzalexins A-F, phytoalexin biosynthesis
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-
?
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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cell culture
Manually annotated by BRENDA team
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OsDTS2 mRNA in leaves is up-regulated by conditions that stimulate phytoalexin biosynthesis
Manually annotated by BRENDA team
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OsDTS2 mRNA is constitutively expressed
Manually annotated by BRENDA team
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
homology model of the second active site of enzyme based on the structure of 5-epiaristolochene synthase from Nicotiana tabacum, Protein Data Bank ID code 5EAT
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A713S
products are isopimaradiene and sandaracopimaradiene
G651V
no change in product
V717L
no change in product
W679L/Y686H/A713S/V717L
products are isopimaradiene and sandaracopimaradiene
Y686H
no change in product
Y686H/A713S
main products are isopimaradiene and sandaracopimaradiene
Y686H/A713S A713S
main products are isopimaradiene and sandaracopimaradiene
Y686H/A713S/V717L
products are isopimaradiene and sandaracopimaradiene
additional information
swapping of residues 568–640 of isopimaradiene synhase to corresponding residues 560-632 of levopimaradiene/abietadiene synthase results in complete reversion of the product profiles of the two enzymes