Information on EC 4.2.3.31 - ent-pimara-9(11),15-diene synthase

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The expected taxonomic range for this enzyme is: Streptomyces

EC NUMBER
COMMENTARY
4.2.3.31
-
RECOMMENDED NAME
GeneOntology No.
ent-pimara-9(11),15-diene synthase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ent-copalyl diphosphate = ent-pimara-9(11),15-diene + diphosphate
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ent-copalyl-diphosphate diphosphate-lyase [ent-pimara-9(11),15-diene-forming]
This enzyme is involved in the biosynthesis of the diterpenoid viguiepinol and requires Mg2+, Co2+, Zn2+ or Ni2+ for activity.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain KO-3988
-
-
Manually annotated by BRENDA team
strain KO-3988
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ent-copalyl diphosphate
pimara-9(11),15-diene + diphosphate
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
no substrates: farnesyl diphosphate, geranylgeranyl diphosphate, terpentecin diphosphate
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Co2+
-
1 mM, 100% of the activity with Mg2+
Ni2+
-
1 mM, 57% of the activity with Mg2+
Zn2+
-
1 mM, 59% of the activity with Mg2+
Mg2+
-
absolutely required, optimal concentraion 1 mM
additional information
-
no or very weak activity in presence of Mn2+, Ca2+, Cu2+ and Fe2+
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
5 mM, required for full activity
Tween-80
-
0.1%, required for full activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00026
ent-copalyl diphosphate
-
30°C, pH 7.0
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0014
ent-copalyl diphosphate
-
30°C, pH 7.0
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
-
0.05 M Tris, pH 7.0
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
35000
-
gel filtration
680552
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
monomer
-
x * 35000, SDS-PAGE
monomer
-
x * 35000, SDS-PAGE
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
35
-
pH 5.5, stable for at least 1 h
680552