Information on EC 4.2.3.31 - ent-pimara-9(11),15-diene synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Streptomyces

EC NUMBER
COMMENTARY
4.2.3.31
-
RECOMMENDED NAME
GeneOntology No.
ent-pimara-9(11),15-diene synthase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ent-copalyl diphosphate = ent-pimara-9(11),15-diene + diphosphate
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ent-copalyl-diphosphate diphosphate-lyase [ent-pimara-9(11),15-diene-forming]
This enzyme is involved in the biosynthesis of the diterpenoid viguiepinol and requires Mg2+, Co2+, Zn2+ or Ni2+ for activity.
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain KO-3988
-
-
Manually annotated by BRENDA team
strain KO-3988
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ent-copalyl diphosphate
pimara-9(11),15-diene + diphosphate
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
no substrates: farnesyl diphosphate, geranylgeranyl diphosphate, terpentecin diphosphate
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Co2+
-
1 mM, 100% of the activity with Mg2+
Ni2+
-
1 mM, 57% of the activity with Mg2+
Zn2+
-
1 mM, 59% of the activity with Mg2+
Mg2+
-
absolutely required, optimal concentraion 1 mM
additional information
-
no or very weak activity in presence of Mn2+, Ca2+, Cu2+ and Fe2+
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
5 mM, required for full activity
Tween-80
-
0.1%, required for full activity
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.00026
-
ent-copalyl diphosphate
-
30°C, pH 7.0
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0014
-
ent-copalyl diphosphate
-
30°C, pH 7.0
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
0.05 M Tris, pH 7.0
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
35000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
monomer
-
x * 35000, SDS-PAGE
monomer
-
x * 35000, SDS-PAGE
-
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
35
-
-
pH 5.5, stable for at least 1 h