Information on EC 4.2.3.21 - vetispiradiene synthase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.3.21
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RECOMMENDED NAME
GeneOntology No.
vetispiradiene synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2E,6E)-farnesyl diphosphate = vetispiradiene + diphosphate
show the reaction diagram
the initial internal cyclization produces the monocyclic intermediate germacrene A
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
internal cyclization
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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Sesquiterpenoid and triterpenoid biosynthesis
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sesquiterpenoid phytoalexins biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
trans,trans-farnesyl-diphosphate diphosphate-lyase (cyclizing, vetispiradiene-forming)
The initial internal cyclization produces the monocyclic intermediate germacrene A.
CAS REGISTRY NUMBER
COMMENTARY hide
192465-18-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
elicitin-challenged and Ralstonia solancearum-inoculated enzyme
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Manually annotated by BRENDA team
no activity in Solanum tuberosum
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
trans,trans-farnesyl diphosphate
vetispiradiene + diphosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
trans,trans-farnesyl diphosphate
vetispiradiene + diphosphate
show the reaction diagram
Q9XIZ0
infection of Phytophthora infestans with potato tubers causes transient increase in the transcript level of vetispiradiene synthase. Wound-induced expression of the squalene synthase is suppressed in favor of the expression of vetispiradiene synthase
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?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002 - 0.005
trans,trans-farnesyl diphosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.04
trans,trans-farnesyl diphosphate
Hyoscyamus muticus
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recombinant enzyme
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant vetispiradiene synthase, a chimeric 5-epi-aristolochene synthase and a chimeric sesquiterpene cyclase possessing multifunctional epi-aristolochene and vetispiradiene activity
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli. Highest level of sesquiterpene production occurs when the enzymes are expressed in strain DH5alpha from the trc promoter of the high-copy plasmid pTrc99A in M9 medium supplemented with 0.2% v/v glycerol at 30°C
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expression of 3 cDNA clones in Escherichia coli
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hexahistidyl-tagged enzyme expressed in Escherichia coli, genes for vetispiradiene synthase, a chimeric 5-epi-aristolochene synthase and a chimeric sesquiterpene cyclase possessing multifunctional epi-aristolochene and vetispiradiene activity
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
after 24 h incubation in the dark, the gene coding for vetispiradiene synthase pvs1 is significantly induced incultured varieties Favorita and Longshu-3. The genes coding for hydroxymethylglutaryl coenzyme A reductase and squalene synthase remain relatively stable in wild species Solanum chacoense and variety Longshu-3