Information on EC 4.2.3.147 - pimaradiene synthase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.3.147
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RECOMMENDED NAME
GeneOntology No.
pimaradiene synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(+)-copalyl diphosphate = pimara-8(14),15-diene + diphosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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Diterpenoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(+)-copalyl diphosphate-lyase (pimara-8(14),15-diene-forming)
Isolated from the plants Pinus banksiana (jack pine) and Pinus contorta (lodgepole pine).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-copalyl diphosphate
aphidicol-15-ene + phosphate
show the reaction diagram
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products of mutant T696I are about 20% pimara-7,15-diene plus 80% aphidicol-15-ene, no product of wild-type
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?
(+)-copalyl diphosphate
pimara-7,15-diene + diphosphate
show the reaction diagram
-
-
product of wild-type
-
?
(+)-copalyl diphosphate
pimara-8(14),15-diene + diphosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(+)-copalyl diphosphate
pimara-8(14),15-diene + diphosphate
show the reaction diagram
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
T696I
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mutation leads to a dramatic increase in reaction complexity. Product outcome is shifted from the tricyclic pimara-7,15-diene to a rearranged tetracycle, aphidicol-15-ene. Products are about 20% pimara-7,15-diene plus 80% aphidicol-15-ene