Information on EC 4.2.3.144 - geranyllinalool synthase

Word Map on EC 4.2.3.144
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.3.144
-
RECOMMENDED NAME
GeneOntology No.
geranyllinalool synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
geranylgeranyl diphosphate + H2O = (E,E)-geranyllinalool + diphosphate
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
(E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene biosynthesis
-
-
Diterpenoid biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
geranylgeranyl diphosphate diphosphate-lyase ((E,E)-geranyllinalool-forming)
The enzyme is a component of the herbivore-induced indirect defense system. The product, (E,E)-geranyllinalool, is a precursor to the volatile compound 4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT), which is released by many plants in response to damage.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
KJ755868
GenBank
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
KJ755870
GenBank
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
geranylgeranyl diphosphate + H2O
(E,E)-geranyllinalool + diphosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
geranylgeranyl diphosphate + H2O
(E,E)-geranyllinalool + diphosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
highest activity with 10 mM Mg2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0187 - 0.0315
geranylgeranyl diphosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6.85 - 7.53
geranylgeranyl diphosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.24 - 0.37
geranylgeranyl diphosphate
231
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
KJ755868
constitutive expression
Manually annotated by BRENDA team
additional information
KJ755870
enzyme is not expressed in any tissue under normal growth
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme is not expressed in any tissue under normal growth but is induced in leaves by alamethicin and methyl jasmonate treatments
KJ755870
highly coordinated herbivore-induced expression of geranyllinalool synthase with CYP82G1 (At3g25180, a cytochrome P450 monooxygenase of the Arabidopsis CYP82 family, is responsible for the breakdown of the C20-precursor (E,E)-geranyllinalool to the insect-induced C16-homoterpene (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene (TMTT)) in leaves depending on the F-box protein COI-1
-
in leaves, expression is induced by methyl jasmonate
KJ755868
is expressed in response to Pseudomonas syringae inoculation or intoxication of Arabidopsis leaves with copper sulfate
transcription is upregulated under conditions that induce (E,E)-geranyllinalool and 4,8,12-trimethyltrideca-1,3,7,11-tetraene synthesis, including infestation of plants with larvae of the moth Plutella xylostella and treatment with the fungal peptide alamethicin or the octadecanoid mimic coronalon. Induction requires jasmonic acid but is independent from salicylic acid or ethylene
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
contrary to wild-type, transgenic Lotus japonicus plants expressing isoform TPS2 produce (E,E)-geranyllinalool and (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene. Transgenic Nicotiana tabacum expressing isoform TPS2 produces (E,E)-geranyllinalool but not (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene. In olfactory assays, the generalist predatory mite Neoseiulus californicus but not the specialist Phytoseiulus persimilis is attracted to uninfested, transgenic Lotus japonicus plants expressing TPS2 over wild-type plants. The specialist Phytoseiulus persimilis is more strongly attracted by the transgenic plants infested with spider mites than by infested wild-type plants