Information on EC 4.2.3.143 - kunzeaol synthase

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The expected taxonomic range for this enzyme is: Thapsia garganica

EC NUMBER
COMMENTARY hide
4.2.3.143
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RECOMMENDED NAME
GeneOntology No.
kunzeaol synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2E,6E)-farnesyl diphosphate + H2O = kunzeaol + diphosphate
show the reaction diagram
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-
-
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SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (kunzeaol forming)
Isolated from the root of the plant Thapsia garganica. The enzyme also produces germacrene D, bicyclogermacrene and traces of other sesquiterpenoids. See EC 4.2.3.77, (+)-germacrene D synthase and EC 4.2.3.100, bicyclogermacrene synthase.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
enzyme is involved in thapsigargin biosynthesis
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate + H2O
kunzeaol + diphosphate
show the reaction diagram
-
-
i.e. 6beta-hydroxygermacra-1(10),4-diene, (1R,2E,6E,10R)-3,7-dimethyl-10-isopropylcyclodeca-2,6-dienol, major product, plus germacrene D, bicyclogermacrene and two more minor products
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
-
100 mM, complete loss of activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0026
(2E,6E)-farnesyl diphosphate
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pH 7.5, 28°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.03
(2E,6E)-farnesyl diphosphate
Thapsia garganica
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pH 7.5, 28°C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
64700
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x * 64700, calculated, x * 65000, SDS-PAGE
65000
-
x * 64700, calculated, x * 65000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 64700, calculated, x * 65000, SDS-PAGE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Saccharomyces cerevisiae and Escherichia coli
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